{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:13:20Z","timestamp":1785428000499,"version":"3.56.0"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"15","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2025,8,5]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Early reperfusion after ST\u2010segment\u2013elevation myocardial infarction is essential for limiting infarct size. However, reperfusion injury can lead to progressive microvascular obstruction (MVO), reducing myocardial blood flow (MBF), aggravating ischemia and myocardial necrosis. We hypothesized that SuperSaturated Oxygen (SSO\n              <jats:sub>2<\/jats:sub>\n              ) Therapy may reduce infarct size by alleviating MVO, so we evaluated the effects of SSO\n              <jats:sub>2<\/jats:sub>\n              on the time course of MBF and MVO in a preclinical ischemia\/reperfusion model.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Twelve swine surviving 90\u2009minutes of balloon\u2010induced anterior ST\u2010segment\u2013elevation myocardial infarction and 15\u2010minute auto\u2010reperfusion, were assigned to 120 minutes of SSO\n              <jats:sub>2<\/jats:sub>\n              (n=6) or further 120 minutes of auto\u2010reperfusion (control, n=6). Microspheres were injected into the left ventricle at multiple time points to assess MBF, calculated as the total blood flow within areas at risk normalized to the total flow within remote zones. An angiography\u2010derived index of microcirculatory resistance was analyzed. MVO and infarct zones were identified using thioflavin\u2010S and 2,3,5\u2010triphenyl tetrazolium chloride staining and quantified with ImageJ software.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              SSO\n              <jats:sub>2<\/jats:sub>\n              Therapy significantly reduced MVO compared with controls (4.64% versus 13.00% of left ventricular area;\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              &lt;0.001) and improved myocardial salvage index (MSI, 64.76% versus 43.11%;\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              =0.03). MBF was significantly higher in the SSO\n              <jats:sub>2<\/jats:sub>\n              group compared with controls at the end of therapy (1.1 versus 0.59;\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              =0.03). In the controls, following initial hyperemia, flow decreased significantly at 165, 195, and 225\u2009minutes (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              =0.01). Conversely, the SSO\n              <jats:sub>2<\/jats:sub>\n              group showed no significant decrease in MBF in the same interval (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              =0.38). Median angiography\u2010derived index of microcirculatory resistance values showed a nonsignificant trend of reduced final microvascular resistance in the SSO\n              <jats:sub>2<\/jats:sub>\n              group only.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              In a translational ST\u2010segment\u2013elevation myocardial infarction model, SSO\n              <jats:sub>2<\/jats:sub>\n              prevented a reduction in MBF during the 120\u2010minute reperfusion period, with significantly increased MBF at the end of the experiment. MBF improvement was translated to a 64% relative reduction in the extent of MVO, and a 50% relative increase in the myocardial salvage index.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.124.038891","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T12:04:54Z","timestamp":1752753894000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Intracoronary SuperSaturated Oxygen Therapy on Myocardial Blood Flow, Microvascular Obstruction, and Myocardial Salvage in ST\u2010Segment\u2013Elevation Myocardial Infarction in Swine Ischemia\/Reperfusion Model"],"prefix":"10.1161","volume":"14","author":[{"given":"Pedro H. C.","family":"Melo","sequence":"first","affiliation":[{"name":"Cardiovascular Research Foundation","place":["Orangeburg, USA"]},{"name":"Hospital S\u00edrio\u2010Liban\u00eas","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateusz","family":"Kachel","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Foundation","place":["Orangeburg, USA"]},{"name":"American Heart of Poland","place":["Katowice, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3782-5383","authenticated-orcid":false,"given":"Kacper","family":"Nowak","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Foundation","place":["Orangeburg, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Cheng","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Foundation","place":["Orangeburg, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3197-8410","authenticated-orcid":false,"given":"Paul","family":"Wilburn","sequence":"additional","affiliation":[{"name":"ZOLL TherOx, Inc.","place":["Irvine, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald","family":"Blum","sequence":"additional","affiliation":[{"name":"ZOLL TherOx, Inc.","place":["Irvine, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramu","family":"Perumal","sequence":"additional","affiliation":[{"name":"ZOLL TherOx, Inc.","place":["Irvine, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey L.","family":"Creech","sequence":"additional","affiliation":[{"name":"ZOLL TherOx, Inc.","place":["Irvine, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1146-0542","authenticated-orcid":false,"given":"Gilles","family":"Barone\u2010Rochette","sequence":"additional","affiliation":[{"name":"University Hospital","place":["Grenoble, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6258-0544","authenticated-orcid":false,"given":"Robert A.","family":"Kloner","sequence":"additional","affiliation":[{"name":"Keck School of Medicine of University of Southern California","place":["Los Angeles, USA"]},{"name":"Huntington Medical Research Institutes","place":["Pasadena, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2399-8320","authenticated-orcid":false,"given":"Juan F.","family":"Granada","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Foundation","place":["Orangeburg, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3995-2466","authenticated-orcid":false,"given":"Daniel","family":"Burkhoff","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Foundation","place":["Orangeburg, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6915-5033","authenticated-orcid":false,"given":"Grzegorz L.","family":"Kaluza","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Foundation","place":["Orangeburg, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1112570"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahjo.2021.100044"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/1074248411405990"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.101.25.2981"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107898"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.62.5.945"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.80.6.1846"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/1074248411407637"},{"key":"e_1_3_1_10_2","first-page":"160","article-title":"Aqueous oxygen hyperbaric reperfusion in a porcine model of myocardial infarction","volume":"14","author":"Spears JR","year":"2002","unstructured":"Spears JR, Henney C, Prcevski P, Xu R, Li L, Brereton GJ, DiCarli M, Spanta A, Crilly R, Sulaiman AM, et\u00a0al. 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