{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T07:10:05Z","timestamp":1793689805702,"version":"4.5.1"},"reference-count":40,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"18","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2025,9,16]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">The link between lymph node (LN) function and atherosclerosis is unclear, especially at the single\u2010cell level. This study explores the relationship between pericarotid LNs and plaque stability using single\u2010cell transcriptome analysis.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods<\/jats:title>\n            <jats:p xml:lang=\"en\">We enrolled 106 patients with severe internal carotid artery stenosis undergoing carotid endarterectomy. We recorded baseline clinical data and pericarotid LN size, and analyzed plaque composition to assess its association with enlarged LNs. Single\u2010cell transcriptome and T\/B\u2010cell receptor sequencing were used to profile pericarotid LNs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Patients with symptomatic carotid atherosclerosis had more enlarged pericarotid LNs (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              &lt;0.001), indicating plaque instability. This was characterized by increased inflammation, greater CD68\n              <jats:sup>+<\/jats:sup>\n              macrophage infiltration (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              &lt;0.001), and reduced fibrous content (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              &lt;0.001). Single\u2010cell analysis identified 3 main cellular compartments: lymphocytes, myeloid cells, and stromal cells. Enlarged LNs had significantly more naive B and T cells (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              &lt;0.001), suggesting heightened immune response. Macrophages and neutrophils may help in cholesterol transport and degradation, while stromal cells may regulate the immune microenvironment by influencing T\u2010cell tolerance and lymphocyte trafficking.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Enlarged pericarotid LNs are potential biomarkers for plaque instability and recent ischemic events. This study provides a comprehensive single\u2010cell transcriptomic landscape of human LNs in atherosclerosis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.124.039777","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T11:52:14Z","timestamp":1757591534000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Single\u2010Cell Transcriptomics of Human Pericarotid Lymph Nodes Indicating Stability of Carotid Atherosclerotic Plaques"],"prefix":"10.1161","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1943-586X","authenticated-orcid":false,"given":"Tao","family":"Sun","sequence":"first","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanzhao","family":"Wu","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohao","family":"Liu","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9761-0838","authenticated-orcid":false,"given":"Zhiwei","family":"Xue","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengchen","family":"Yu","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4466-9083","authenticated-orcid":false,"given":"Bo","family":"Mao","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9869-8237","authenticated-orcid":false,"given":"Mengtao","family":"Han","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7545-3328","authenticated-orcid":false,"given":"Xiangjun","family":"Liang","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5632-2449","authenticated-orcid":false,"given":"Lu","family":"Ling","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8793-1866","authenticated-orcid":false,"given":"Nanlin","family":"You","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0222-0297","authenticated-orcid":false,"given":"Longxin","family":"Ji","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4771-366X","authenticated-orcid":false,"given":"Han","family":"Liu","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]},{"name":"Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6604-4364","authenticated-orcid":false,"given":"Chenyang","family":"Xu","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]},{"name":"Huaihe Hospital of Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5797-3590","authenticated-orcid":false,"given":"Zhen","family":"Qin","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0816-8161","authenticated-orcid":false,"given":"Peng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0114-1281","authenticated-orcid":false,"given":"Donghai","family":"Wang","sequence":"additional","affiliation":[{"name":"Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science, Shandong University","place":["Jinan, China"]},{"name":"Shandong Key Laboratory of Brain Health and Function Remodeling","place":["Jinan, China"]},{"name":"Qilu Hospital of Shandong University Dezhou Hospital","place":["Dezhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2015.03.013"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00584-1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2018.07.014"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61728-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0590-4"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.301137"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.313591"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI63685"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0b013e32830d5f09"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0281-x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.118.310802"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.122.060799"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvaa244"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.316770"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.120.315373"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.123.322590"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.atv.20.5.1177"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14466"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-00955-7"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020277"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2017.1319544"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2014.11.003"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000486"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312585111"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.04.006"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00575"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.308648"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.122.317781"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-013-9406-1"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-019-0169-2"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehac605"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.312513"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.01.007"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2085"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.320090"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s44161-023-00218-w"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-018-0106-9"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19113479"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03459-5"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.124.039777","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T03:57:24Z","timestamp":1758167844000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.124.039777"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,16]]},"references-count":40,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2025,9,16]]}},"alternative-id":["10.1161\/JAHA.124.039777"],"URL":"https:\/\/doi.org\/10.1161\/jaha.124.039777","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,16]]},"assertion":[{"value":"2024-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e039777"}}