{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:44:02Z","timestamp":1784036642613,"version":"3.55.0"},"reference-count":45,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"15","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2025,8,5]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Vascular dysfunction contributes to postoperative organ injury. Exposure to high concentrations of oxygen during surgery is common and may impair vascular function. We tested the hypothesis that hyperoxia during cardiac surgery impairs vascular function compared with normoxia.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods<\/jats:title>\n            <jats:p xml:lang=\"en\">We recruited and randomly assigned patients having elective cardiac surgery to hyperoxia or normoxia during surgery, measured endothelium\u2010mediated vasodilation via brachial artery flow\u2010mediated dilation and fingertip pulse amplitude tonometry (reactive hyperemia index), assessed endothelium\u2010dependent, endothelium\u2010independent, and heme\u2010independent soluble guanylyl cyclase activator\u2013induced vasodilation ex\u00a0vivo in mediastinal fat arterioles using wire myography, and quantified plasma markers of vascular function and oxidative stress.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Two hundred participants completed the study. Oxygen treatment did not affect flow\u2010mediated dilation (primary outcome,\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              =0.377) or reactive hyperemia index (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              =0.898). In isolated mediastinal fat arterioles, however, hyperoxia impaired endothelium\u2010independent relaxation (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              &lt;0.001) but not endothelium\u2010dependent relaxation (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              =0.759) or heme\u2010independent soluble guanylyl cyclase activation (\n              <jats:italic toggle=\"no\">P<\/jats:italic>\n              =0.650). Hyperoxia also increased plasma plasminogen activator inhibitor\u20101 postoperatively but not e\u2010selectin or syndecan\u20101. Hyperoxia increased intraoperative concentrations of F\n              <jats:sub>2<\/jats:sub>\n              \u2010isoprostanes and isofurans, which were associated with plasminogen activator inhibitor\u20101 but not other measurements of vascular function.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Among adults receiving cardiac surgery, intraoperative hyperoxia did not affect endothelium\u2010dependent vasodilation but impaired endothelium\u2010independent vasodilation, likely via soluble guanylyl cyclase heme oxidation. Soluble guanylyl cyclase is a potential therapeutic target to enhance vascular function.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Registration<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              URL:\n              <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.clinicaltrials.gov\">https:\/\/www.clinicaltrials.gov<\/jats:ext-link>\n              ; Unique identifier: NCT02361944.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.125.041778","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T12:07:02Z","timestamp":1752754022000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of Oxygen on Perioperative Vascular Function: A Randomized Clinical Trial"],"prefix":"10.1161","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2622-4267","authenticated-orcid":false,"given":"Marcos G.","family":"Lopez","sequence":"first","affiliation":[{"name":"Vanderbilt University Medical Center","place":["Nashville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7890-4665","authenticated-orcid":false,"given":"Arvind K.","family":"Pandey","sequence":"additional","affiliation":[{"name":"Brigham and Women\u2019s Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra","family":"Hennessy","sequence":"additional","affiliation":[{"name":"Vanderbilt University Medical Center","place":["Nashville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9261-5875","authenticated-orcid":false,"given":"Christopher G.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Vanderbilt 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