{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:56:02Z","timestamp":1785891362847,"version":"3.56.0"},"reference-count":36,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2026,3,17]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Neuroprotective strategies as primary or ancillary treatment could ameliorate the significant mortality and disability associated with ischemic stroke. Nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol (NLGM1) were previously shown to ameliorate brain injury when given before arterial occlusion in mice. We aimed to test the hypothesis that NLGM1 given following transient intraluminal filament and photothrombotic (PT) middle cerebral artery occlusion (MCAO) will attenuate acute and chronic stroke injury in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p xml:lang=\"en\">Twelve\u2010week\u2010old C57BL\/6 mice underwent MCAO for 45\u2009minutes and were then injected with 1 or 3 doses of saline or NLGM1 (1 or 2\u2009mg IV) after occlusion. Neurologic deficit score and brain infarct area were measured after 48\u2009hours. Separate mice underwent PT occlusion of the distal lateral middle cerebral artery trunk followed by injection of 1 or 2 doses saline or NLGM1 (1 or 2\u2009mg IV) postocclusion. Motor and behavior testing was performed at 21, 30, 45, and 90\u2009days after PT MCAO.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Following intraluminal MCAO, single\u2010dose NLGM1\u2010treated mice exhibited reduced neurological impairment (neurologic deficit score 3.6\u00b10.2, 2.6\u00b10.3, 2.4\u00b10.3 at 0, 1, 2\u2009mg, respectively;\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.05 versus control) and reduced infarct area (29.8\u00b12.9%, 15.0\u00b11.5%, 13.0\u00b11.2%;\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.001 versus control) with similar results with multiple dosing. Following PT MCAO, day 21 latency to fall (rotarod test), day 30 corner test, day 45 adhesive tape removal, and day 90 novel object recognition were improved in single\u2010dose NLGM1\u2010treated mice versus controls with improved corner and adhesive tape removal tests with multiple dosing. There were no significant differences in outcomes between 1 versus 2\u2009mg NLGM1 treatments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Treatment of mice with NLGM1 resulted in reduced brain infarct size acutely following intraluminal MCAO and improved motor and behavior function acutely and chronically following intraluminal and PT MCAO. NLGM1 represents a promising novel neuroprotective agent in stroke.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.125.042974","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T10:38:26Z","timestamp":1773139106000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["Monosialoganglioside\u2010Containing Nanoliposomes Protect Against Acute and Chronic Ischemic Stroke Injury"],"prefix":"10.1161","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2410-574X","authenticated-orcid":false,"given":"Saif","family":"Ahmad","sequence":"first","affiliation":[{"name":"Department of Translational Neuroscience\/Neurosurgery, Barrow Neurological Institute\/St. Joseph\u2019s Hospital and Medical Center","place":["Phoenix, 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USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaleb T.","family":"Morrow","sequence":"additional","affiliation":[{"name":"Phoenix Veterans Affairs Health Care System","place":["Phoenix, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8549-8570","authenticated-orcid":false,"given":"Sudhanshu","family":"Raikwar","sequence":"additional","affiliation":[{"name":"Phoenix Veterans Affairs Health Care System","place":["Phoenix, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2011-0955","authenticated-orcid":false,"given":"Naseem","family":"Akhter","sequence":"additional","affiliation":[{"name":"Department of Translational Neuroscience\/Neurosurgery, Barrow Neurological Institute\/St. Joseph\u2019s Hospital and Medical Center","place":["Phoenix, 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