{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T11:45:23Z","timestamp":1782992723795,"version":"3.54.5"},"reference-count":68,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2026,3,17]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Avocado consumption has been associated with improvements in diet quality and cardiometabolic risk factors, but effects on serum metabolite profiles remain underexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Secondary analysis was conducted using untargeted metabolomics to assess fasting serum metabolite profiles at baseline (preintervention) and 6 months (postintervention) in a subset of participants with abdominal obesity from the HAT (Habitual Diet and Avocado Trial), who were randomized to the avocado group (n\u2009=\u2009120; 70% women; 49\u2009\u00b1\u200913\u2009years of age; body mass index 33.1\u2009\u00b1\u20095.7\u2009kg\/m\n                      <jats:sup>2<\/jats:sup>\n                      ). Pre\u2013post intervention changes in metabolites, cardiometabolic risk factors (blood pressure, lipid profile, glucose, insulin, high\u2010sensitivity C\u2010reactive protein), visceral adipose tissue volume, and hepatic fat fraction were evaluated using Wilcoxon tests. Multilevel partial\u2010least squares discriminant analysis, accounting for within\u2010subject correlation was used to examine metabolite changes associated with avocado intake, and multiple regression assessed metabolite\u2010cardiometabolic risk factor associations, adjusting for covariates (age, sex, body mass index, smoking status, physical activity, energy intake) and multiple testing (false discovery rate &lt;0.1).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Plasma\n                      <jats:styled-content style=\"fixed-case\" toggle=\"no\">low\u2010density lipoprotein cholesterol<\/jats:styled-content>\n                      (\u22126%), systolic (\u22123%), and diastolic (\u22122%) blood pressure decreased, whereas visceral adipose tissue volume increased (3%) postintervention (all\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      \u2009&lt;\u20090.05). We identified 30 primary (sugar acids\/alcohols, amino\/carboxylic\/hydroxy acids, indoles, xenobiotics) and 45 lipid\u2010related metabolites (fatty acids, cholesteryl esters, glycerolipids, glycerophospholipids, sphingolipids) as key drivers of separation between pre\u2013post intervention time points (variable importance in projection &gt;1). Significant but weak to modest associations (multiple\u2010\n                      <jats:italic toggle=\"no\">R<\/jats:italic>\n                      \u2009=\u20090.21\u20130.52) were observed between 96 predominantly lipid\u2010related metabolites and visceral adipose tissue volume, plasma triglycerides, and total cholesterol concentrations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Avocado intake was associated with subtle shifts in serum metabolites related to lipid, carbohydrate, and amino acid metabolism, with weak effects on visceral adipose tissue volume, plasma triglycerides, and total cholesterol concentrations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Registration<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      URL:\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.clinicaltrials.gov\">https:\/\/www.clinicaltrials.gov<\/jats:ext-link>\n                      ; Unique identifier: NCT03528031.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.125.044144","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T11:34:41Z","timestamp":1773401681000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["Serum Metabolite Profiles in Adults With Abdominal Obesity in Response to Consuming 1 Avocado Daily for 6 Months: An Exploratory Analysis"],"prefix":"10.1161","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7037-9779","authenticated-orcid":false,"given":"Nirupa R.","family":"Matthan","sequence":"first","affiliation":[{"name":"Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2875-638X","authenticated-orcid":false,"given":"Ecaterina","family":"Duscova","sequence":"additional","affiliation":[{"name":"Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5367-5867","authenticated-orcid":false,"given":"Gregory","family":"Matuszek","sequence":"additional","affiliation":[{"name":"Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6012-4900","authenticated-orcid":false,"given":"Penny M.","family":"Kris\u2010Etherton","sequence":"additional","affiliation":[{"name":"The Pennsylvania State University","place":["University Park, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3914-0353","authenticated-orcid":false,"given":"Kristina S.","family":"Petersen","sequence":"additional","affiliation":[{"name":"The Pennsylvania State University","place":["University Park, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6367-4406","authenticated-orcid":false,"given":"Sujatha","family":"Rajaram","sequence":"additional","affiliation":[{"name":"Loma Linda University School of Public Health","place":["Loma Linda, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8662-4310","authenticated-orcid":false,"given":"Zhaoping","family":"Li","sequence":"additional","affiliation":[{"name":"David Geffen School of Medicine at UCLA","place":["Los Angeles, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Reboussin","sequence":"additional","affiliation":[{"name":"Wake Forest University School of Medicine","place":["Winston\u2010Salem, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9063-9785","authenticated-orcid":false,"given":"Joan","family":"Sabate","sequence":"additional","affiliation":[{"name":"Loma Linda University School of Public Health","place":["Loma Linda, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1053-9478","authenticated-orcid":false,"given":"Alice H.","family":"Lichtenstein","sequence":"additional","affiliation":[{"name":"Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","unstructured":"2020\u20132025 Dietary Guidelines for Americans. https:\/\/www.dietaryguidelines.gov\/sites\/default\/files\/2020\u201012\/dietary_guidelines_for_americans_2020\u20102025.pdf."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jand.2018.05.019"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3945\/jn.111.157222"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jand.2020.08.076"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu13124305"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyw319"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-017-1469-0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","unstructured":"Elsahoryi NA Neville CE Patterson CC Linden GJ Moitry M Biasch K Kee F Amouyel P Bongard V Dallongeville J et\u00a0al. Association between overall fruit and vegetable intake and fruit and vegetable sub\u2010types and blood pressure: the PRIME study (prospective epidemiological study of myocardial infarction). 2021;125:557\u2013567. doi: 10.1017\/S0007114520001518","DOI":"10.1017\/S0007114520001518"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afae043"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(02)98858-6"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nutrit\/nuz104"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.120.048996"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu13124376"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jand.2022.12.008"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2891-12-1"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.41189"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu14132744"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqx078"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacl.2015.10.011"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxac126"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2022.03.005"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxab187"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/jaha.122.025657"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cdnut.2024.102079"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.110.000950"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.288.20.2569"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu13113760"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13070800"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00035.2018"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","unstructured":"Reboussin DM Kris\u2010Etherton PM Lichtenstein AH Li Z Sabate J Matthan NR Petersen K Rajaram S Vitolins M Ford N. The design and rationale of a multi\u2010center randomized clinical trial comparing one avocado per day to usual diet: the habitual diet and avocado trial (HAT). 2021;110:106565. doi: 10.1016\/j.cct.2021.106565","DOI":"10.1016\/j.cct.2021.106565"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajcnut.2024.08.002"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb3004s114"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/11530084_18"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2551"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-395"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/18.6.499"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.4324\/9780203774441"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12937-024-00915-7"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.123.030497"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/dme.12120"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu10091287"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114522002690"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2004.09.010"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/cdn\/nzz068"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqy131"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12263-020-00670-4"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.144501"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjnut.2023.07.013"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","unstructured":"Ford NA Spagnuolo P Kraft J Bauer E. Nutritional composition of hass avocado pulp. 2023;12:2516. doi: 10.3390\/foods12132516","DOI":"10.3390\/foods12132516"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.13351"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","unstructured":"Wang M Yu P Chittiboyina AG Chen D Zhao J Avula B Wang Y\u2010H Khan IA. Characterization quantification and quality assessment of avocado (Persea americana Mill.) oils. 2020;25:1453. doi: 10.3390\/molecules25061453","DOI":"10.3390\/molecules25061453"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.10.022"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxaa219"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1039\/d4fo03806a"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cdnut.2024.104451"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c08953"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2025.2501195"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejcn.1601634"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1017\/s0007114598001585"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.3390\/metabo15090602"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajcnut.2023.03.024"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22115695"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1161\/jaha.118.010810"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/oby.22654"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-58430-8"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1097\/PN1099.0000000000000007"},{"key":"e_1_3_2_68_2","first-page":"887","article-title":"Lysophosphatidylcholine and lysophosphatidylinosiol\u2010novel promissing signaling molecules and their possible therapeutic activity","volume":"71","author":"Drzazga A","year":"2014","unstructured":"Drzazga A, Sowi\u0144ska A, Kozio\u0142kiewicz M. Lysophosphatidylcholine and lysophosphatidylinosiol\u2010novel promissing signaling molecules and their possible therapeutic activity. Acta Pol Pharm. 2014;71:887\u2013899.","journal-title":"Acta Pol Pharm"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.C400011-JLR200"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.125.044144","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T11:13:28Z","timestamp":1782990808000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.125.044144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,17]]},"references-count":68,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,3,17]]}},"alternative-id":["10.1161\/JAHA.125.044144"],"URL":"https:\/\/doi.org\/10.1161\/jaha.125.044144","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,17]]},"assertion":[{"value":"2025-06-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e044144"}}