{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:57:51Z","timestamp":1789786671908,"version":"4.0.1"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2026,1,6]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Periodontitis is a chronic inflammatory condition with infectious origin that affects the tissues supporting the teeth. Increasing epidemiological evidence suggests that periodontitis is a risk factor for ischemic stroke with associated adverse outcomes. However, the underlying mechanism of this association remains incompletely elucidated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      We used a C57BL\/6J mice model of ischemic stroke induced by transitory occlusion of the middle cerebral artery in the presence or absence of ligature\u2010induced periodontitis using\n                      <jats:italic toggle=\"no\">Porphyromonas gingivalis<\/jats:italic>\n                      \u2010soaked ligatures. Stroke severity was evaluated through infarct volume, sensorimotor deficit, blood\u2013brain barrier (BBB) integrity, and markers of systemic and brain inflammation. The direct effect of\n                      <jats:italic toggle=\"no\">P gingivalis<\/jats:italic>\n                      on BBB endothelial cells was further explored in\u00a0vitro.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Mice with\n                      <jats:italic toggle=\"no\">P gingivalis<\/jats:italic>\n                      \u2010associated periodontitis showed a significant exacerbation of stroke severity: larger infarct volume, more severe sensorimotor deficit, greater BBB disruption, and increased brain neutrophil infiltration compared with sham. Systemic inflammation was also markedly elevated. Intravenous administration of\n                      <jats:italic toggle=\"no\">P gingivalis<\/jats:italic>\n                      alone, without gingival injury, before transitory occlusion of the middle cerebral artery was sufficient to amplify brain inflammation and stroke lesions. In\u00a0vitro\n                      <jats:italic toggle=\"no\">P gingivalis,<\/jats:italic>\n                      through its gingipain proteases, directly impaired BBB integrity by increasing endothelial permeability and disrupting tight\u2010junction proteins.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Our findings demonstrate that\n                      <jats:italic toggle=\"no\">P gingivalis<\/jats:italic>\n                      \u2010associated periodontitis worsens ischemic stroke outcome both indirectly by enhancing systemic and brain inflammation and directly via BBB disruption. These results highlight periodontitis as a modifiable risk factor and potential therapeutic target for improving stroke prognosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.125.045997","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T10:22:50Z","timestamp":1767090170000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Unveiling the Impact of\n                    <i>Porphyromonas gingivalis<\/i>\n                    \u2010Associated Periodontitis on Stroke Outcome in Mice"],"prefix":"10.1161","volume":"15","author":[{"given":"Devy","family":"Diallo","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, INSERM U1148, LVTS","place":["Paris, France"]},{"name":"Laboratoire d\u2019Excellence INFLAMEX","place":["Paris, 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