{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T11:45:22Z","timestamp":1782992722552,"version":"3.54.5"},"reference-count":68,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2026,1,20]]},"abstract":"\n Background<\/jats:title>\n \n Caldesmon is an actomyosin\u2010tropomyosin\u2013binding protein with pleiotropic functions that generally acts as an inhibitor of actomyosin\n ATPase<\/jats:styled-content>\n activity. This study aims to explore the role of\n caldesmon<\/jats:styled-content>\n in regulation of preglomerular vascular tone.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Expression of\n caldesmon<\/jats:styled-content>\n , myosin light chain, and \u03b1\u2010smooth muscle actin was assessed by western blot of interlobar arteries (IAs) from young and old mice or human renal arteries from patients who underwent nephrectomy or tumor enucleation.\n IAs<\/jats:styled-content>\n were obtained from wild\u2010type or heterozygous mice, carrying a mutant allele that ablates both\n caldesmon<\/jats:styled-content>\n isoforms (\n caldesmon<\/jats:styled-content>\n +\/\u2212<\/jats:sup>\n ). In\n IAs<\/jats:styled-content>\n , the vasoreactivity to \u03b1\n 1<\/jats:sub>\n \u2010agonist phenylephrine, the soluble guanylate cyclase activator cinaciguat, and inhibitors of\n RhoA<\/jats:styled-content>\n \u2010associated protein kinase\n Y27632<\/jats:styled-content>\n or p21\u2010activated protein kinase 1\n IPA\u20103<\/jats:styled-content>\n were tested by wire myography.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Caldesmon was expressed in all preglomerular vessels of mice and humans. In\n caldesmon<\/jats:styled-content>\n +\/\u2212<\/jats:sup>\n IAs of young mice<\/jats:styled-content>\n ,\n caldesmon<\/jats:styled-content>\n protein was 50% less than in\n IAs<\/jats:styled-content>\n of wild\u2010type\n mice<\/jats:styled-content>\n . In\n IAs of old mice<\/jats:styled-content>\n ,\n caldesmon<\/jats:styled-content>\n was less than in\n IAs of young mice<\/jats:styled-content>\n and not significantly different between\n caldesmon<\/jats:styled-content>\n +\/\u2212<\/jats:sup>\n and wild\u2010type.\n Caldesmon<\/jats:styled-content>\n mutation did not affect phenylephrine\u2010induced contraction or cinaciguat sensitivity but induced a rightward shift of\n Y27632<\/jats:styled-content>\n \u2010induced relaxation in\n IAs of young and old mice<\/jats:styled-content>\n and a reduction in\n IPA\u20103\u2013<\/jats:styled-content>\n induced relaxation by 50% only in\n IAs of young mice<\/jats:styled-content>\n . In human renal arteries,\n caldesmon<\/jats:styled-content>\n expression negatively correlated with age and the presence of renal inflammation. In the inflammation group, a reduction in estimated glomerular filtration rate was detected.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n This study provides cumulative evidence that caldesmon is a marker of age and inflammation\u2010related changes in preglomerular vascular function. Our work supports the view that in the renal vasculature,\n caldesmon<\/jats:styled-content>\n expression is age dependent and determines its sensitivity to the\n RhoA<\/jats:styled-content>\n \u2010associated protein kinase\/p21\u2010activated protein kinase\n 1<\/jats:styled-content>\n regulatory pathway.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.125.046679","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T13:03:26Z","timestamp":1768827806000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Regulation of Vascular Tone of Preglomerular Renal Vasculature by Caldesmon"],"prefix":"10.1161","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6971-2802","authenticated-orcid":false,"given":"Galyna","family":"Pryymachuk","sequence":"first","affiliation":[{"name":"Brandenburg Medical School Theodor Fontane","place":["Neuruppin, 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