{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T10:07:49Z","timestamp":1789553269837,"version":"build-2803163510"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2026,6,16]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Disrupted cerebral blood flow is suggested to contribute to secondary brain injury after cardiac arrest (CA). This study aimed to investigate cerebral microcirculation after return of spontaneous circulation (ROSC) following asphyxial CA. We hypothesized that contraction of pericytes after ROSC compromises cerebral capillary blood flow.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p xml:lang=\"en\">Transgenic C57BL\/6NRj mice (n=19) of both sexes were studied. Mice were expressing tdTomato as a fluorescent reporter under the control of the platelet\u2010derived growth factor receptor \u03b2 promoter to label pericytes. Chronic cranial windows were implanted 3\u2009weeks before the experiment. Four minutes of asphyxial CA was followed by cardiopulmonary resuscitation. Two\u2010photon microscopy assessed cerebral hemodynamics in the same cohort of mice at both 3 and 24\u2009hours after ROSC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Of 13 mice in the CA group, 9 achieved ROSC; 6 and 5 mice survived to 3 and 24\u2009hours after ROSC, respectively. Arterial blood pressure was similar between groups 3 and 24\u2009hours after ROSC. At 3\u2009hours after ROSC, pial arteries and penetrating arterioles were constricted in the CA group compared with sham (arteriole diameter, 12.2\u2009\u03bcm [95% CI, 10.9\u201313.4] versus 15.6\u2009\u03bcm [95% CI, 13.8\u201317.5] in CA and sham;\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      =0.003). Similarly, first\u2010 and second\u2010 to third\u2010order capillaries showed reduced diameters 3\u2009hours after ROSC (first\u2010order diameter, 3.9\u2009\u03bcm [95% CI, 3.5\u20134.2] versus 5.3\u2009\u03bcm [95% CI, 4.8\u20135.9] in CA and sham;\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      =0.002). The vasoconstriction was associated with slower red blood cell velocities throughout the capillary network (for second\u2010 to third\u2010order capillaries upstream from venule; 0.78\u2009mm\/s [95% CI, 0.46\u20131.09] versus 2.14\u2009mm\/s [95% CI, 1.73\u20132.55];\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.001) and increased capillary flow stalling. Artery\u2010to\u2010vein mean transit time was increased and relative transit\u2010time heterogeneity was decreased 3\u2009hours after ROSC. By 24\u2009hours after ROSC, vessel diameters, blood flow velocity, transit time, and capillary stalling were not different compared with sham.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Cerebrovascular vasospasm 3\u2009hours after ROSC was associated with impaired cerebral microcirculation and increased capillary flow stalling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.125.047582","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T11:55:56Z","timestamp":1781006156000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["Postresuscitation Cerebral Vasospasm and Capillary Failure After Experimental Asphyxial Cardiac Arrest"],"prefix":"10.1161","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5431-4409","authenticated-orcid":false,"given":"Christian","family":"Staehr","sequence":"first","affiliation":[{"name":"Aarhus University Hospital","place":["Aarhus, Denmark"]},{"name":"Aarhus University","place":["Aarhus, Denmark"]},{"name":"University of the Sunshine Coast","place":["Maroochydore, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilie Munch","family":"Johannsen","sequence":"additional","affiliation":[{"name":"Aarhus University","place":["Aarhus, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3117-2676","authenticated-orcid":false,"given":"Victoria","family":"Hinkley","sequence":"additional","affiliation":[{"name":"University of the Sunshine Coast","place":["Maroochydore, Australia"]},{"name":"University of Queensland","place":["St Lucia, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7241-6131","authenticated-orcid":false,"given":"Asger","family":"Granfeldt","sequence":"additional","affiliation":[{"name":"Aarhus University Hospital","place":["Aarhus, Denmark"]},{"name":"Aarhus University","place":["Aarhus, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7509-0720","authenticated-orcid":false,"given":"Eugenio","family":"Guti\u00e9rrez\u2010Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Aarhus University","place":["Aarhus, Denmark"]},{"name":"Aarhus University","place":["Aarhus, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-021-06548-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2018.01.027"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.32.1.128"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2017.00174"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000000027"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.92.9.2572"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1655953"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01709335"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200008000-00030"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2007.06.028"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X221113022"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17744717"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(96)00073-9"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2017.08.218"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccrj.2024.06.001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-016-0934-5"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.34861"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17732229"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00793-2"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1922755117"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-061121-040127"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1306031"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2022"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13165"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.123.029527"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awad401"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108260"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20914179"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17721666"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00872.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/alz.70023"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-72039-1"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16631560"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.21037\/qims-20-920"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10827-009-0159-1"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2024.110216"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1978.tb17305.x"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200008000-00031"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.29.3.705"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612825666181120155953"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20220796"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.120.032737"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.114.005888"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1113\/ep087519"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X241305562"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.762843"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.272.6.H2622"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00318570"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-015-0557-x"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-19086-z"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00373.2021"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/apha.70030"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resplu.2023.100511"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.125.047582","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:45:31Z","timestamp":1789551931000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.125.047582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,16]]},"references-count":53,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,6,16]]}},"alternative-id":["10.1161\/JAHA.125.047582"],"URL":"https:\/\/doi.org\/10.1161\/jaha.125.047582","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,16]]},"assertion":[{"value":"2025-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e047582"}}