{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:38:38Z","timestamp":1792647518246,"version":"4.5.0"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"20","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Immune\u2010mediated inflammatory responses exacerbate brain injury and affect the prognosis in acute ischemic stroke.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p xml:lang=\"en\">We characterized longitudinal complement activation after acute ischemic stroke using serial plasma samples from 10 patients and 8 controls. Initial markers of classical (C1q), lectin (mannose\u2010binding lectin), and alternative (complement factor Bb) pathways, as well as regulatory (complement factor H) and downstream markers (C3a, C5a, and C5b\u20109) were measured by ELISA at 9 time points.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">Patients with stroke showed broad complement activation, with increased C1q, mannose\u2010binding lectin, complement factor Bb, C3a, C5a, and C5b\u20109, and reduced complement factor H compared with controls. Complement components showed distinct temporal patterns during follow\u2010up. Complement factor Bb levels were lower in patients with unfavorable functional outcome and in those with moderate\u2010to\u2010severe stroke. C1q and C3a trajectories differed according to functional outcome, whereas C3a trajectories also differed according to stroke severity. C1q trajectories differed according to age group and infarct volume.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">These findings suggest dynamic, pathway\u2010specific complement activation after acute ischemic stroke and highlight the need for larger studies to clarify its prognostic and therapeutic relevance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.125.048042","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:26:52Z","timestamp":1792150012000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Temporal Dynamics of Complement Activation and Outcome in Patients With Acute Ischemic Stroke"],"prefix":"10.1161","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6332-5935","authenticated-orcid":false,"given":"Yan","family":"Wang","sequence":"first","affiliation":[{"name":"Amsterdam University Medical Center","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Neuroscience","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8403-7109","authenticated-orcid":false,"given":"Willeke F.","family":"Westendorp","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Neuroscience","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3246-5083","authenticated-orcid":false,"given":"Merijn W.","family":"Bijlsma","sequence":"additional","affiliation":[{"name":"Amsterdam Neuroscience","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam University Medical Center","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9828-4459","authenticated-orcid":false,"given":"Michael W. T.","family":"Tanck","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5324-1748","authenticated-orcid":false,"given":"Wing Kit","family":"Man","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Neuroscience","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7775-7843","authenticated-orcid":false,"given":"Matthijs C.","family":"Brouwer","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Neuroscience","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6609-2206","authenticated-orcid":false,"given":"Inge A.","family":"Mulder","sequence":"additional","affiliation":[{"name":"Amsterdam Neuroscience","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam University Medical Center Amsterdam","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Cardiovascular Sciences","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8284-982X","authenticated-orcid":false,"given":"Jonathan M.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Neuroscience","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Cardiovascular Sciences","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4571-044X","authenticated-orcid":false,"given":"Diederik","family":"van de Beek","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Neuroscience","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1474-4422(19)30034-1"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010014"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/strokeaha.122.038867"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-015-0464-8"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201904"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.Res.0000232544.90675.42"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-024-03316-z"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jstrokecerebrovasdis.2020.105363"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.112.103051"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021338"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-8682-0"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11020339"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/strokeaha.120.031715"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.0000000000006717"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2014.06.039"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.2197-17.2018"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci144348"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113703"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-022-02278-1"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/s2665-9913(20)30341-6"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-015-0372-y"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci57522"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2008.07.044"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-018-0658-3"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.125.048042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:53:43Z","timestamp":1792644823000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.125.048042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,20]]},"references-count":24,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2026,10,20]]}},"alternative-id":["10.1161\/JAHA.125.048042"],"URL":"https:\/\/doi.org\/10.1161\/jaha.125.048042","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,20]]},"assertion":[{"value":"2025-11-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e048042"}}