{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T05:20:13Z","timestamp":1787721613774,"version":"build-2784847793"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"13","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2026,7,7]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Acute and chronic heart failure secondary to myocardial infarction (MI) and cardiac ischemia\u2013reperfusion injury (IRI) are leading causes of death in ischemic heart disease. A mouse model is indispensable for investigating MI and IRI, and the development of reliable mouse MI and IRI models is essential for advancing research in this field. The clear early diagnostic criteria for confirming successful induction of MI and IRI in mice remain lacking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p xml:lang=\"en\">Adult C57BL\/6J background mice underwent left anterior descending coronary artery ligation to induce acute MI, or ligation followed by reperfusion to induce IRI. The success of the MI and IRI model establishment was confirmed by 2,3,5\u2010triphenyltetrazolium chloride staining and echocardiography. Electrocardiography was used to monitor the electric activity in the mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Electrocardiography demonstrated that ST\u2010segment elevation in ECG lead II and corrected QTc interval prolongation at 30\u2009minutes following left anterior descending ligation as 2 key early indicators of successful MI. Echocardiography analysis revealed that the magnitude of ST\u2010segment elevation strongly correlated with the left anterior descending ligation site, where a more proximal ligation produced a greater ST\u2010segment elevation amplitude and more severe ischemia. In IRI models, ST\u2010segment elevation typically resolved and returned to baseline within 20\u2009minutes of reperfusion. This study developed quantifiable early diagnostic criteria for successful MI and IRI induction based on characteristic ECG changes. These quantifiable ECG parameters provide early diagnostic standards that can significantly streamline and optimize modeling procedures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.125.048262","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T10:33:24Z","timestamp":1782210804000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Development of Electrocardiography Standards for Evaluating Myocardial Infarction and Ischemia\u2013Reperfusion Injury in Mice"],"prefix":"10.1161","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6044-3999","authenticated-orcid":false,"given":"Peili","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Jiao Tong University School of Medicine","place":["Shanghai, 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