{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T22:28:31Z","timestamp":1788560911283,"version":"build-2803163510"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background and Purpose\u2014<\/jats:italic>\n            <\/jats:bold>\n            Retinal ischemia is a major cause of visual impairment and is associated with a high risk of subsequent ischemic stroke. The retina and its projections are easily accessible for experimental procedures and functional evaluation. We created and characterized a mouse model of global and transient retinal ischemia and provide a comprehensive chronologic profile of some genes that display altered expression during ischemia.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods\u2014<\/jats:italic>\n            <\/jats:bold>\n            Ischemia and reperfusion were assessed by observing flat-mounted retinas after systemic fluorescein injection. The temporal pattern of gene expression modulation was evaluated by quantitative reverse transcription\u2013polymerase chain reaction from the occurrence of unilateral 30-minute pterygopalatine artery occlusion until 4 weeks after reperfusion. Electroretinograms evaluated functional sequelae 4 weeks after the ischemic episode and were correlated with histologic lesions.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            This model is the first to reproduce the features of transient monocular amaurosis fugax resulting from ophthalmic artery occlusion. The histologic structure was roughly conserved, but functional lesions affected ganglion cells, inner nuclear layer cells, and photoreceptor cells. We observed an early and strong upregulation of c\n            <jats:italic>-fos<\/jats:italic>\n            , c\n            <jats:italic>-jun, Cox-2, Hsp70<\/jats:italic>\n            , and\n            <jats:italic>Gadd34<\/jats:italic>\n            gene expression and a late decrease in\n            <jats:italic>Hsp70<\/jats:italic>\n            transcript levels.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            A murine model of transient retinal ischemia was successfully developed that exhibited the characteristic upregulation of immediate-early genes and persistent functional deficits. The model should prove useful for investigating mechanisms of injury in genetically altered mice and for testing novel neuroprotective drugs.\n          <\/jats:p>","DOI":"10.1161\/strokeaha.107.499319","type":"journal-article","created":{"date-parts":[[2007,11,2]],"date-time":"2007-11-02T00:48:54Z","timestamp":1193964534000},"page":"3237-3244","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":19,"title":["Novel Mouse Model of Monocular Amaurosis Fugax"],"prefix":"10.1161","volume":"38","author":[{"given":"Dominique Claude","family":"Lelong","sequence":"first","affiliation":[{"name":"From the Centre de Recherche The\u0301rapeutique en Ophtalmologie-CERTO (D.C.L., K.B., E.P., J.L., M.M., M.A.), Faculte\u0301 de Me\u0301decine Paris Descartes; Laboratoire de Ge\u0301ne\u0301tique Mole\u0301culaire (I.B., I.L., M.V.), INSERM U745, Faculte\u0301 des Sciences Pharmaceutiques et Biologiques; and Service de biostatistiques et d\u2019informatique me\u0301dicale (J.-P.J.), Faculte\u0301 de Me\u0301decine Paris Descartes, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Bieche","sequence":"additional","affiliation":[{"name":"From the Centre de Recherche The\u0301rapeutique en Ophtalmologie-CERTO (D.C.L., K.B., E.P., J.L., M.M., M.A.), Faculte\u0301 de Me\u0301decine Paris Descartes; Laboratoire de Ge\u0301ne\u0301tique Mole\u0301culaire (I.B., I.L., M.V.), INSERM U745, Faculte\u0301 des Sciences Pharmaceutiques et Biologiques; and Service de biostatistiques et d\u2019informatique me\u0301dicale (J.-P.J.), Faculte\u0301 de Me\u0301decine Paris Descartes, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Perez","sequence":"additional","affiliation":[{"name":"From the Centre de Recherche The\u0301rapeutique en Ophtalmologie-CERTO (D.C.L., K.B., E.P., J.L., M.M., M.A.), Faculte\u0301 de Me\u0301decine Paris Descartes; Laboratoire de Ge\u0301ne\u0301tique Mole\u0301culaire (I.B., I.L., M.V.), INSERM U745, Faculte\u0301 des Sciences Pharmaceutiques et Biologiques; and Service de biostatistiques et d\u2019informatique me\u0301dicale (J.-P.J.), Faculte\u0301 de Me\u0301decine Paris Descartes, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Bigot","sequence":"additional","affiliation":[{"name":"From the Centre de Recherche The\u0301rapeutique en Ophtalmologie-CERTO (D.C.L., K.B., E.P., J.L., M.M., M.A.), Faculte\u0301 de Me\u0301decine Paris Descartes; Laboratoire de Ge\u0301ne\u0301tique Mole\u0301culaire (I.B., I.L., M.V.), INSERM U745, Faculte\u0301 des Sciences Pharmaceutiques et Biologiques; and Service de biostatistiques et d\u2019informatique me\u0301dicale (J.-P.J.), Faculte\u0301 de Me\u0301decine Paris Descartes, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Leemput","sequence":"additional","affiliation":[{"name":"From the Centre de Recherche The\u0301rapeutique en Ophtalmologie-CERTO (D.C.L., K.B., E.P., J.L., M.M., M.A.), Faculte\u0301 de Me\u0301decine Paris Descartes; Laboratoire de Ge\u0301ne\u0301tique Mole\u0301culaire (I.B., I.L., M.V.), INSERM U745, Faculte\u0301 des Sciences Pharmaceutiques et Biologiques; and Service de biostatistiques et d\u2019informatique me\u0301dicale (J.-P.J.), Faculte\u0301 de Me\u0301decine Paris Descartes, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Laurendeau","sequence":"additional","affiliation":[{"name":"From the Centre de Recherche The\u0301rapeutique en Ophtalmologie-CERTO (D.C.L., K.B., E.P., J.L., M.M., M.A.), Faculte\u0301 de Me\u0301decine Paris Descartes; Laboratoire de Ge\u0301ne\u0301tique Mole\u0301culaire (I.B., I.L., M.V.), INSERM U745, Faculte\u0301 des Sciences Pharmaceutiques et Biologiques; and Service de biostatistiques et d\u2019informatique me\u0301dicale (J.-P.J.), Faculte\u0301 de Me\u0301decine Paris Descartes, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Vidaud","sequence":"additional","affiliation":[{"name":"From the Centre de Recherche The\u0301rapeutique en Ophtalmologie-CERTO (D.C.L., K.B., E.P., J.L., M.M., M.A.), Faculte\u0301 de Me\u0301decine Paris Descartes; Laboratoire de Ge\u0301ne\u0301tique Mole\u0301culaire (I.B., I.L., M.V.), INSERM U745, Faculte\u0301 des Sciences Pharmaceutiques et Biologiques; and Service de biostatistiques et d\u2019informatique me\u0301dicale (J.-P.J.), Faculte\u0301 de Me\u0301decine Paris Descartes, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Jais","sequence":"additional","affiliation":[{"name":"From the Centre de Recherche The\u0301rapeutique en Ophtalmologie-CERTO (D.C.L., K.B., E.P., J.L., M.M., M.A.), Faculte\u0301 de Me\u0301decine Paris Descartes; 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