{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:46:45Z","timestamp":1791190005923,"version":"4.1.0"},"reference-count":39,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background and Purpose\u2014<\/jats:italic>\n            <\/jats:bold>\n            In animal models of stroke, functional improvement has been obtained after stem cell transplantation. Successful therapy depends largely on achieving a robust and targeted cell engraftment, with intraarterial (IA) injection being a potentially attractive route of administration. We assessed the suitability of laser Doppler flow (LDF) signal measurements and magnetic resonance (MR) imaging for noninvasive dual monitoring of targeted IA cell delivery.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods\u2014<\/jats:italic>\n            <\/jats:bold>\n            Transient cerebral ischemia was induced in adult Wistar rats (n=25) followed by IA or intravenous (IV) injection of mesenchymal stem cells (MSCs) labeled with superparamagnetic iron oxide. Cell infusion was monitored in real time with transcranial laser Doppler flowmetry while cellular delivery was assessed with MRI in vivo (4.7T) and ex vivo (9.4T).\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            Successful delivery of magnetically labeled MSCs could be readily visualized with MRI after IA but not IV injection. IA stem cell injection during acute stroke resulted in a high variability of cerebral engraftment. The amount of LDF reduction during cell infusion (up to 80%) was found to correlate well with the degree of intracerebral engraftment, with low LDF values being associated with significant morbidity.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            High cerebral engraftment rates are associated with impeded cerebral blood flow. Noninvasive dual-modality imaging enables monitoring of targeted cell delivery, and through interactive adjustment may improve the safety and efficacy of stem cell therapy.\n          <\/jats:p>","DOI":"10.1161\/strokeaha.107.502047","type":"journal-article","created":{"date-parts":[[2008,3,7]],"date-time":"2008-03-07T02:25:35Z","timestamp":1204856735000},"page":"1569-1574","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":354,"title":["Dual-Modality Monitoring of Targeted Intraarterial Delivery of Mesenchymal Stem Cells After Transient Ischemia"],"prefix":"10.1161","volume":"39","author":[{"given":"Piotr","family":"Walczak","sequence":"first","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assaf A.","family":"Gilad","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota A.","family":"Kedziorek","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus","family":"Ruiz-Cabello","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randell G.","family":"Young","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark F.","family":"Pittenger","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.M.","family":"van Zijl","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Huang","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff W.M.","family":"Bulte","sequence":"additional","affiliation":[{"name":"From the Neurosection, Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science (P.W., A.A.G., D.A.K., J.R.-C., P.C.M.v.Z., J.W.M.B.), the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (P.W., A.A.G., D.A.K., J.R.-C., J.W.M.B.), the Departments of Neurosurgery (J.Z., J.H.), Chemical and Biomolecular Engineering (J.W.M.B.), and Biomedical Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, Baltimore, Md;..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(79)90472-4"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1997.6730"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199504273321702"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.55.4.565"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20501"},{"key":"e_1_3_2_6_2","first-page":"624","volume":"37","year":"2006","unstructured":"de Freitas GR, Mendonca MLF, Bezerra DC, Silva SA, Falcao CH, Gonzales CS, Moreira RC, Haddad AF, Tuche FA, Santos DP, Andre C, Mesquita CT, Oliveira AA, Elia V, Dohmann HJ, Borojevic R, Mendez-Otero R, Dohmann H. 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