{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T14:47:07Z","timestamp":1774795627917,"version":"3.50.1"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background and Purpose\u2014<\/jats:italic>\n            <\/jats:bold>\n            Human growth and transformation-dependent protein (HGTD-P) is a new proapoptotic protein and an effector of cell death induced by hypoxia-ischemia (HI). The function of HGTD-P has been investigated in human prostate cancer cells and mouse neurons cultured in vitro. However, whether HGTD-P is involved in regulating the apoptosis of rat neurons is not clear, and the relevance of HGTD-P in HI animal models is still unknown. Therefore, in the present study, we tried to elucidate the role that HGTD-P plays in apoptosis of rat neurons subjected to HI, both in culture and in the developing rat brain in vivo.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods\u2014<\/jats:italic>\n            <\/jats:bold>\n            Samples from primary cultured neurons and postnatal day 10 rat brains with HI were collected. RT-PCR, Western blotting, and immunocytochemistry were used to detect the expression and distribution of rat HGTD-P, cleaved caspase 3, and apoptosis- inducing factor (AIF). MTT assay, DAPI, TUNEL, and flowcytometry were used to detect cell viability and apoptosis.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            We found that HI upregulated the mRNA and protein levels of HGTD-P in rat neurons in vitro and in vivo. Antisense oligonucleotides (AS) targeted to HGTD-P inhibited the expression of HGTD-P, thus rescuing neuronal viability and attenuating neuronal apoptosis. In addition, we found that HGTD-P played its proapoptotic role by activating caspase 3 and inducing the translocation of AIF to nuclear.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            Our findings show that HGTD-P plays a proapoptotic role in the developing rat brain after HI and that it may be a potential target in treating HI-induced brain damage.\n          <\/jats:p>","DOI":"10.1161\/strokeaha.109.553644","type":"journal-article","created":{"date-parts":[[2009,6,12]],"date-time":"2009-06-12T10:34:25Z","timestamp":1244802865000},"page":"2843-2848","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":21,"title":["Proapoptotic Role of Human Growth and Transformation-Dependent Protein in the Developing Rat Brain After Hypoxia-Ischemia"],"prefix":"10.1161","volume":"40","author":[{"given":"Yi","family":"Qu","sequence":"first","affiliation":[{"name":"From the Department of Pediatrics (Y.Q., M.M., F.Z., L.Z., D.M.), West China Second University Hospital, Sichuan University, Chengdu, China; and the Department of Neurology (D.M.), University of California, San Francisco."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Mao","sequence":"additional","affiliation":[{"name":"From the Department of Pediatrics (Y.Q., M.M., F.Z., L.Z., D.M.), West China Second University Hospital, Sichuan University, Chengdu, China; and the Department of Neurology (D.M.), University of California, San Francisco."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"From the Department of Pediatrics (Y.Q., M.M., F.Z., L.Z., D.M.), West China Second University Hospital, Sichuan University, Chengdu, China; and the Department of Neurology (D.M.), University of California, San Francisco."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Pediatrics (Y.Q., M.M., F.Z., L.Z., D.M.), West China Second University Hospital, Sichuan University, Chengdu, China; and the Department of Neurology (D.M.), University of California, San Francisco."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dezhi","family":"Mu","sequence":"additional","affiliation":[{"name":"From the Department of Pediatrics (Y.Q., M.M., F.Z., L.Z., D.M.), West China Second University Hospital, Sichuan University, Chengdu, China; and the Department of Neurology (D.M.), University of California, San Francisco."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","updated-by":[{"DOI":"10.1161\/str.0000000000000232","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T00:00:00Z","timestamp":1586476800000},"record-id":"23099"},{"DOI":"10.1161\/str.0000000000000232","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T00:00:00Z","timestamp":1586476800000}}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/STROKEAHA.109.553644","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T13:00:31Z","timestamp":1715605231000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/STROKEAHA.109.553644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["10.1161\/STROKEAHA.109.553644"],"URL":"https:\/\/doi.org\/10.1161\/strokeaha.109.553644","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8]]},"assertion":[{"value":"2009-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-04-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}