{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:25:31Z","timestamp":1790349931660,"version":"4.1.0"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:sec>\n            <jats:title>Background and Purpose\u2014<\/jats:title>\n            <jats:p>The neurovascular unit is a major target of hypoxia-ischemia (HI) injury in the neonatal brain. Although neurons are the cellular target of ischemic preconditioning (IP), vessel tolerance also contributes greatly to protection. Nerves and vessels cross-talk and use common signals during development. Cellular inhibitor of apoptosis 1 (cIAP1) is an important regulator that inhibits apoptosis. This study hypothesized that cIAP1 is a shared molecule underlying IP-mediated neurovascular protection against HI in the neonatal brain.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods\u2014<\/jats:title>\n            <jats:p>In vivo IP was induced by 2-hour reversible occlusion of right carotid artery 24 hours before HI on postpartum day 7 in rat pups. In vitro oxygen\u2013glucose deprivation (OGD) preconditioning was established in SH-SY5Y neuronal cells and in human microvascular endothelial cell-1 vascular endothelial cells. cIAP1 expression was inhibited by cIAP1 small interfering RNA in vivo or by lentivirus-mediated short hairpin RNA in vitro, or was upregulated by the lentiviral expression system.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results\u2014<\/jats:title>\n            <jats:p>IP reduced apoptosis, selectively increased cIAP1 in neurons and vascular endothelial cells, and provided long-term neuroprotection against HI. Intracerebroventricular delivery of cIAP1 small interfering RNA significantly attenuated IP-mediated cIAP1 upregulation and neuroprotection in vivo. In vitro, OGD preconditioning induced cIAP1 and protected against OGD cell death in SH-SY5Y neuronal and human microvascular endothelial cells-1. Knockdown of cIAP1 by lentivirus-mediated short hairpin RNA decreased the protective effect of OGD preconditioning in SH-SY5Y and human microvascular endothelial cell-1, whereas overexpression of cIAP1 by lentivirus protected against OGD in these cells.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>cIAP1 is a shared molecule underlying IP-induced protection in neurons and vascular endothelial cells against HI in the neonatal brain.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/strokeaha.112.677617","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T01:54:44Z","timestamp":1354154084000},"page":"162-169","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":29,"title":["Ischemic Preconditioning Reduces Neurovascular Damage After Hypoxia-Ischemia Via the Cellular Inhibitor of Apoptosis 1 in Neonatal Brain"],"prefix":"10.1161","volume":"44","author":[{"given":"Wan-Ying","family":"Lin","sequence":"first","affiliation":[{"name":"From the Institute of Basic Medical Sciences (W.-Y.L.) and Department of Pediatrics (C.-J.H., C.-C.H.), National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan; and Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan (Y.-C.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Chao","family":"Chang","sequence":"additional","affiliation":[{"name":"From the Institute of Basic Medical Sciences (W.-Y.L.) and Department of Pediatrics (C.-J.H., C.-C.H.), National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan; and Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan (Y.-C.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Jung","family":"Ho","sequence":"additional","affiliation":[{"name":"From the Institute of Basic Medical Sciences (W.-Y.L.) and Department of Pediatrics (C.-J.H., C.-C.H.), National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan; and Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan (Y.-C.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Ching","family":"Huang","sequence":"additional","affiliation":[{"name":"From the Institute of Basic Medical Sciences (W.-Y.L.) and Department of Pediatrics (C.-J.H., C.-C.H.), National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan; and Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan (Y.-C.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","volume-title":"Neurology of the newborn","author":"Volpe JJ","year":"2008","unstructured":"Volpe JJ. 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