{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:35:31Z","timestamp":1790062531284,"version":"4.0.1"},"reference-count":11,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec>\n            <jats:title>Background and Purpose\u2014<\/jats:title>\n            <jats:p>To determine the optimal dose of 2-iminobiotin (2-IB) for the treatment of moderate to severe asphyxia in a neonatal piglet model of hypoxia-ischemia.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods\u2014<\/jats:title>\n            <jats:p>Newborn piglets were subjected to a 30-minute hypoxia-ischemia insult and randomly treated with vehicle or 2-IB (0.1 mg\/kg, 0.2 mg\/kg, or 1.0 mg\/kg). aEEG background and seizure activity were scored after hypoxia-ischemia every 4 h until 24 h and at 48 h and neurobehavioral scores were obtained. Brain tissue was collected and processed for analysis of caspase-3 activity, histology, and tyrosine nitration.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results\u2014<\/jats:title>\n            <jats:p>A dose range of 0.1 to 1.0 mg\/kg\/dose of 2-IB improved short-term outcome as demonstrated by an increased survival with a normal aEEG and decreased nitrotyrosine staining in the 2-IB\u2013treated animals, indicating decreased cellular damage. Neurobehavior, caspase-3 activity in thalamus, and histology scores were not significantly different.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>Based on survival with a normal aEEG, 0.2 mg\/kg 2-IB is likely to be the most appropriate dose for use in future clinical trials in neonates with perinatal hypoxia-ischemia.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/strokeaha.112.677922","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T05:12:07Z","timestamp":1359522727000},"page":"809-811","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":22,"title":["Short-Term Dose\u2013Response Characteristics of 2-Iminobiotin Immediately Postinsult in the Neonatal Piglet After Hypoxia-Ischemia"],"prefix":"10.1161","volume":"44","author":[{"given":"S. Tracey","family":"Bjorkman","sequence":"first","affiliation":[{"name":"From the Centre for Clinical Research, Perinatal Research Centre, University of Queensland, Herston, Queensland, Australia (S.T.B., Z.I., X.F., P.B.C.); the Department of Biostatistics and Research Support, Julius Center for Health Sciences and Primary Care, GA Utrecht, Utrecht, The Netherlands (W.M.v.d.W., K.C.B.R.); and Neurophyxia B.V., DE\u2019s-Hertogenbosch, The Netherlands (C.M.P.C.D.P.-S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Ireland","sequence":"additional","affiliation":[{"name":"From the Centre for Clinical Research, Perinatal Research Centre, University of Queensland, Herston, Queensland, Australia (S.T.B., Z.I., X.F., P.B.C.); the Department of Biostatistics and Research Support, Julius Center for Health Sciences and Primary Care, GA Utrecht, Utrecht, The Netherlands (W.M.v.d.W., K.C.B.R.); and Neurophyxia B.V., DE\u2019s-Hertogenbosch, The Netherlands (C.M.P.C.D.P.-S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyong","family":"Fan","sequence":"additional","affiliation":[{"name":"From the Centre for Clinical Research, Perinatal Research Centre, University of Queensland, Herston, Queensland, Australia (S.T.B., Z.I., X.F., P.B.C.); the Department of Biostatistics and Research Support, Julius Center for Health Sciences and Primary Care, GA Utrecht, Utrecht, The Netherlands (W.M.v.d.W., K.C.B.R.); and Neurophyxia B.V., DE\u2019s-Hertogenbosch, The Netherlands (C.M.P.C.D.P.-S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem M.","family":"van der Wal","sequence":"additional","affiliation":[{"name":"From the Centre for Clinical Research, Perinatal Research Centre, University of Queensland, Herston, Queensland, Australia (S.T.B., Z.I., X.F., P.B.C.); the Department of Biostatistics and Research Support, Julius Center for Health Sciences and Primary Care, GA Utrecht, Utrecht, The Netherlands (W.M.v.d.W., K.C.B.R.); and Neurophyxia B.V., DE\u2019s-Hertogenbosch, The Netherlands (C.M.P.C.D.P.-S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kit C.B.","family":"Roes","sequence":"additional","affiliation":[{"name":"From the Centre for Clinical Research, Perinatal Research Centre, University of Queensland, Herston, Queensland, Australia (S.T.B., Z.I., X.F., P.B.C.); the Department of Biostatistics and Research Support, Julius Center for Health Sciences and Primary Care, GA Utrecht, Utrecht, The Netherlands (W.M.v.d.W., K.C.B.R.); and Neurophyxia B.V., DE\u2019s-Hertogenbosch, The Netherlands (C.M.P.C.D.P.-S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul B.","family":"Colditz","sequence":"additional","affiliation":[{"name":"From the Centre for Clinical Research, Perinatal Research Centre, University of Queensland, Herston, Queensland, Australia (S.T.B., Z.I., X.F., P.B.C.); the Department of Biostatistics and Research Support, Julius Center for Health Sciences and Primary Care, GA Utrecht, Utrecht, The Netherlands (W.M.v.d.W., K.C.B.R.); and Neurophyxia B.V., DE\u2019s-Hertogenbosch, The Netherlands (C.M.P.C.D.P.-S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cacha M.P.C.D.","family":"Peeters-Scholte","sequence":"additional","affiliation":[{"name":"From the Centre for Clinical Research, Perinatal Research Centre, University of Queensland, Herston, Queensland, Australia (S.T.B., Z.I., X.F., P.B.C.); the Department of Biostatistics and Research Support, Julius Center for Health Sciences and Primary Care, GA Utrecht, Utrecht, The Netherlands (W.M.v.d.W., K.C.B.R.); and Neurophyxia B.V., DE\u2019s-Hertogenbosch, The Netherlands (C.M.P.C.D.P.-S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199907000-00002"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2005.06.031"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2554"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.11.067"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.04.119"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2006.07.011"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/jp.2010.93"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2009-2938"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600007"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1159\/000069045"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.str.0000028343.25901.09"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/STROKEAHA.112.677922","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T14:03:16Z","timestamp":1715608996000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/STROKEAHA.112.677922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.1161\/STROKEAHA.112.677922"],"URL":"https:\/\/doi.org\/10.1161\/strokeaha.112.677922","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3]]},"assertion":[{"value":"2012-09-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}