{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T17:21:16Z","timestamp":1773163276307,"version":"3.50.1"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec>\n            <jats:title>Background and Purpose\u2014<\/jats:title>\n            <jats:p>\n              oxLDL (oxidized low-density lipoprotein) has been known for its potential to induce endothelial dysfunction and used as a major serological marker of oxidative stress. Recently, LOX-1 (lectin-like oxidized low-density lipoprotein receptor-1), a lectin-like receptor for oxLDL, has attracted attention in studies of neuronal apoptosis and stroke. We aim to investigate the impact of\n              <jats:italic>LOX-1<\/jats:italic>\n              -deficiency on spontaneous hypertension-related brain damage in the present study.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods\u2014<\/jats:title>\n            <jats:p>\n              We generated a\n              <jats:italic>LOX-1<\/jats:italic>\n              deficient strain on the genetic background of stroke-prone spontaneously hypertensive rat (SHRSP), an animal model of severe hypertension and spontaneous stroke. In this new disease model with stroke-proneness, we monitored the occurrence of brain abnormalities with and without salt loading by multiple procedures including\n              <jats:italic>T<\/jats:italic>\n              <jats:sub>\n                <jats:italic>2<\/jats:italic>\n              <\/jats:sub>\n              weighted magnetic resonance imaging and also explored circulatory miRNAs as diagnostic biomarkers for cerebral ischemic injury by microarray analysis.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results\u2014<\/jats:title>\n            <jats:p>\n              Both\n              <jats:italic>T<\/jats:italic>\n              <jats:sub>\n                <jats:italic>2<\/jats:italic>\n              <\/jats:sub>\n              weighted magnetic resonance imaging abnormalities and physiological parameter changes could be detected at significantly delayed timing in\n              <jats:italic>LOX-1<\/jats:italic>\n              knockout rats compared with wild-type SHRSP, in either case of normal rat chow and salt loading (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.005 in all instances; n=11\u201320 for SHRSP and n=13\u201323 for\n              <jats:italic>LOX-1<\/jats:italic>\n              knockout rats). There were no significant differences in the form of magnetic resonance imaging findings between the strains. A number of miRNAs expressed in the normal rat plasma, including rno-miR-150-5p and rno-miR-320-3p, showed significant changes after spontaneous brain damage in SHRSP, whereas the corresponding changes were modest or almost unnoticeable in\n              <jats:italic>LOX-1<\/jats:italic>\n              knockout rats. There appeared to be the lessening of correlation of postischemic miRNA alterations between the injured brain tissue and plasma in\n              <jats:italic>LOX-1<\/jats:italic>\n              knockout rats.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>\n              Our data show that deficiency of LOX-1 has a protective effect on spontaneous brain damage in a newly generated\n              <jats:italic>LOX-1<\/jats:italic>\n              -deficient strain of SHRSP. Further, our analysis of miRNAs as biomarkers for ischemic brain damage supports a potential involvement of LOX-1 in blood brain barrier disruption after cerebral ischemia.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Visual Overview\u2014<\/jats:title>\n            <jats:p>\n              An online\n              <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"visual overview\">visual overview<\/jats:ext-link>\n              is available for this article.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/strokeaha.120.029421","type":"journal-article","created":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T09:00:25Z","timestamp":1589360425000},"page":"1835-1843","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":25,"title":["LOX-1 (Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1) Deletion Has Protective Effects on Stroke in the Genetic Background of Stroke-Prone Spontaneously Hypertensive Rat"],"prefix":"10.1161","volume":"51","author":[{"given":"Yi-Qiang","family":"Liang","sequence":"first","affiliation":[{"name":"From the Department of Gene Diagnostics and Therapeutics (Y.-Q.L., Y.M., M.I., T.A., H.S., M.T., F.T., N.K.), National Center for Global Health and Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akemi","family":"Kakino","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathophysiology, Shinshu University School of Medicine (A.K., T.S.)"},{"name":"Institute for Biomedical Sciences, Shinshu University (A.K., T.S.)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunari","family":"Matsuzaka","sequence":"additional","affiliation":[{"name":"From the Department of Gene Diagnostics and Therapeutics (Y.-Q.L., Y.M., M.I., T.A., H.S., M.T., F.T., N.K.), National Center for Global Health and Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoji","family":"Mashimo","sequence":"additional","affiliation":[{"name":"Institute of Medical Science, The University of Tokyo (T.M.)"},{"name":"Institute of Experimental Animal Sciences Graduate School of Medicine, Osaka University (T.M.)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Isono","sequence":"additional","affiliation":[{"name":"From the Department of Gene Diagnostics and Therapeutics (Y.-Q.L., Y.M., M.I., T.A., H.S., M.T., F.T., N.K.), National Center for Global Health and Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohisa","family":"Akamatsu","sequence":"additional","affiliation":[{"name":"From the Department of Gene Diagnostics and Therapeutics (Y.-Q.L., Y.M., M.I., T.A., H.S., M.T., F.T., N.K.), National Center for Global Health and Medicine"},{"name":"Department of Pediatrics (T.A.), National Center for Global Health and Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Shimizu","sequence":"additional","affiliation":[{"name":"From the Department of Gene Diagnostics and Therapeutics (Y.-Q.L., Y.M., M.I., T.A., H.S., M.T., F.T., N.K.), National Center for Global Health and Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiko","family":"Tajima","sequence":"additional","affiliation":[{"name":"From the Department of Gene Diagnostics and Therapeutics (Y.-Q.L., Y.M., M.I., T.A., H.S., M.T., F.T., N.K.), National Center for Global Health and Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehito","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Iwate University (T.K.)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Vascular Physiology, National Cerebral and 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