{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:47:18Z","timestamp":1790434038974,"version":"4.1.0"},"reference-count":11,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n            <jats:title>Background and Purpose:<\/jats:title>\n            <jats:p>Small extracellular vesicles (sEVs) obtained from mesenchymal stromal cells (MSCs) were shown to induce ischemic neuroprotection in mice by modulating the brain infiltration of leukocytes and, specifically polymorphonuclear neutrophils. So far, effects of MSC-sEVs were only studied in young ischemic rodents. We herein examined the effects of MSC-sEVs in aged mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>\n              Male and female C57Bl6\/j mice (8\u201310 weeks or 15\u201324 months) were exposed to transient intraluminal middle cerebral artery occlusion. Vehicle or sEVs (equivalent of 2\u00d710\n              <jats:sup>6<\/jats:sup>\n              MSCs) were intravenously administered. Neurological deficits, ischemic injury, blood-brain barrier integrity, brain leukocyte infiltration, and blood leukocyte responses were evaluated over up to 7 days.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>MSC-sEV delivery reduced neurological deficits, infarct volume, brain edema, and neuronal injury in young and aged mice of both sexes, when delivered immediately postreperfusion or with 6 hours delay. MSC-sEVs decreased leukocyte and specifically polymorphonuclear neutrophil, monocyte, and macrophage infiltrates in ischemic brains of aged mice. In peripheral blood, the number of monocytes and activated T cells was significantly reduced by MSC-sEVs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>MSC-sEVs induce postischemic neuroprotection and anti-inflammation in aged mice.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/strokeaha.121.035821","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T10:00:54Z","timestamp":1638352854000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":75,"title":["Postischemic Neuroprotection Associated With Anti-Inflammatory Effects by Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles in Aged Mice"],"prefix":"10.1161","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0213-9642","authenticated-orcid":true,"given":"Chen","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurology (C.W., A.M.Y., C.K., D.M.H.), University Hospital Essen, Germany."},{"name":"Center for Translational and Behavioral Neurosciences (C.W., A.M.Y., C.K., D.M.H.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8230-2218","authenticated-orcid":true,"given":"Verena","family":"B\u00f6rger","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine (V.B., T.T., O.S., F.M., N.F., B.G.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6863-7612","authenticated-orcid":true,"given":"Ayan","family":"Mohamud Yusuf","sequence":"additional","affiliation":[{"name":"Department of Neurology (C.W., A.M.Y., C.K., D.M.H.), University Hospital Essen, Germany."},{"name":"Center for Translational and Behavioral Neurosciences (C.W., A.M.Y., C.K., D.M.H.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8659-8610","authenticated-orcid":true,"given":"Tobias","family":"Tertel","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine (V.B., T.T., O.S., F.M., N.F., B.G.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1686-0323","authenticated-orcid":true,"given":"Oumaima","family":"Stambouli","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine (V.B., T.T., O.S., F.M., N.F., B.G.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Murke","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine (V.B., T.T., O.S., F.M., N.F., B.G.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7053-5532","authenticated-orcid":true,"given":"Nico","family":"Freund","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine (V.B., T.T., O.S., F.M., N.F., B.G.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Kleinschnitz","sequence":"additional","affiliation":[{"name":"Department of Neurology (C.W., A.M.Y., C.K., D.M.H.), University Hospital Essen, Germany."},{"name":"Center for Translational and Behavioral Neurosciences (C.W., A.M.Y., C.K., D.M.H.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Herz","sequence":"additional","affiliation":[{"name":"Department of Pediatrics I (J.H.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5534-6055","authenticated-orcid":true,"given":"Matthias","family":"Gunzer","sequence":"additional","affiliation":[{"name":"Institute of Experimental Immunology and Imaging (M.G.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4574-8605","authenticated-orcid":true,"given":"Aurel","family":"Popa-Wagner","sequence":"additional","affiliation":[{"name":"Center of Experimental and Clinical Medicine, University of Medicine and Pharmacy, Craiova, Romania (A.P.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten R.","family":"Doeppner","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Medicine G\u00f6ttingen, Germany (T.R.D.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2446-948X","authenticated-orcid":true,"given":"Bernd","family":"Giebel","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine (V.B., T.T., O.S., F.M., N.F., B.G.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0198-3152","authenticated-orcid":true,"given":"Dirk M.","family":"Hermann","sequence":"additional","affiliation":[{"name":"Department of Neurology (C.W., A.M.Y., C.K., D.M.H.), University Hospital Essen, Germany."},{"name":"Center for Translational and Behavioral Neurosciences (C.W., A.M.Y., C.K., D.M.H.), University Hospital Essen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.27066"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2013.152"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015-0078"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.116.015204"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-018-0126-4"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.028012"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-017-0599-2"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-019-00115-w"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4428-15.2016"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/STROKEAHA.121.035821","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T15:20:51Z","timestamp":1715613651000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/STROKEAHA.121.035821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1161\/STROKEAHA.121.035821"],"URL":"https:\/\/doi.org\/10.1161\/strokeaha.121.035821","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"2021-03-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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