{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:55:07Z","timestamp":1787064907699,"version":"build-2736575974"},"reference-count":12,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Purpose:<\/jats:title>\n                    <jats:p>Neuroprotective strategies for stroke remain inadequate. Nanoliposomes comprised of phosphatidylcholine, cholesterol, and monosialogangliosides (nanoliposomes) induced an antioxidant protective response in endothelial cells exposed to amyloid insults. We tested the hypotheses that nanoliposomes will preserve human neuroblastoma (SH-SY5Y) and human brain microvascular endothelial cells viability following oxygen-glucose deprivation (OGD)\u2013reoxygenation and will reduce injury in mice following middle cerebral artery occlusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>SH-SY5Y and human brain microvascular endothelial cells were exposed to oxygen-glucose deprivation\u2013reoxygenation (3 hours 0.5%\u20131% oxygen and glucose-free media followed by 20-hour ambient air\/regular media) without or with nanoliposomes (300 \u00b5g\/mL). Viability was measured (calcein-acetoxymethyl fluorescence) and protein expression of antioxidant proteins HO-1 (heme oxygenase-1), NQO1 (NAD[P]H quinone dehydrogenase 1), and SOD1 (superoxide dismutase 1) were measured by Western blot. C57BL\/6J mice were treated with saline (n=8) or nanoliposomes (10 mg\/mL lipid, 200 \u00b5L, n=7) while undergoing 60-minute middle cerebral artery occlusion followed by reperfusion. Day 2 postinjury neurological impairment score and infarction size were compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      SH-SY5Y and human brain microvascular endothelial cells showed reduced viability post\u2013oxygen-glucose deprivation\u2013reoxygenation that was reversed by nanoliposomes. Nanoliposomes increased protein expressions of HO-1, NQO1 in both cell types and SOD1 in human brain microvascular endothelial cells. Nanoliposomes-treated mice showed reduced neurological impairment and brain infarct size (18.8\u00b12% versus 27.3\u00b12.3%,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.017) versus controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Nanoliposomes reduced stroke injury in mice subjected to middle cerebral artery occlusion likely through induction of an antioxidant protective response. Nanoliposome is a candidate novel agent for stroke.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/strokeaha.121.037120","type":"journal-article","created":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T04:00:54Z","timestamp":1636344054000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":14,"title":["Nanoliposomes Reduce Stroke Injury Following Middle Cerebral Artery Occlusion in Mice"],"prefix":"10.1161","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2410-574X","authenticated-orcid":true,"given":"Saif","family":"Ahmad","sequence":"first","affiliation":[{"name":"Barrow Neurological Institute, Phoenix, AZ (S.A., A.K., A.F.D.)."},{"name":"Phoenix Veterans Affairs Healthcare System, AZ (S.A., S.T., N.K., D.R.G., J.L., R.Q.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3622-9064","authenticated-orcid":true,"given":"Seth","family":"Truran","sequence":"additional","affiliation":[{"name":"Phoenix Veterans Affairs Healthcare System, AZ (S.A., S.T., N.K., D.R.G., J.L., R.Q.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Karamanova","sequence":"additional","affiliation":[{"name":"Phoenix Veterans Affairs Healthcare System, AZ (S.A., S.T., N.K., D.R.G., J.L., R.Q.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Kindelin","sequence":"additional","affiliation":[{"name":"Barrow Neurological Institute, Phoenix, AZ (S.A., A.K., A.F.D.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Lozoya","sequence":"additional","affiliation":[{"name":"Midwestern University, Glendale, AZ (M.L., V.W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6466-2367","authenticated-orcid":true,"given":"Volkmar","family":"Weissig","sequence":"additional","affiliation":[{"name":"Midwestern University, Glendale, AZ (M.L., V.W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Emerson","sequence":"additional","affiliation":[{"name":"Barrow Neurological Institute, Phoenix, AZ (S.A., A.K., A.F.D.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6558-261X","authenticated-orcid":true,"given":"Daniel R.","family":"Griffiths","sequence":"additional","affiliation":[{"name":"Phoenix Veterans Affairs Healthcare System, AZ (S.A., S.T., N.K., D.R.G., J.L., R.Q.M.)."},{"name":"University of Arizona College of Medicine-Phoenix (D.R.G., T.V., J.L., A.F.D., R.Q.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1706-9533","authenticated-orcid":true,"given":"Tyler","family":"Vail","sequence":"additional","affiliation":[{"name":"University of Arizona College of Medicine-Phoenix (D.R.G., T.V., J.L., A.F.D., R.Q.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4398-6493","authenticated-orcid":true,"given":"Jonathan","family":"Lifshitz","sequence":"additional","affiliation":[{"name":"Phoenix Veterans Affairs Healthcare System, AZ (S.A., S.T., N.K., D.R.G., J.L., R.Q.M.)."},{"name":"University of Arizona College of Medicine-Phoenix (D.R.G., T.V., J.L., A.F.D., R.Q.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6670-5607","authenticated-orcid":true,"given":"Andrew F.","family":"Ducruet","sequence":"additional","affiliation":[{"name":"University of Arizona College of Medicine-Phoenix (D.R.G., T.V., J.L., A.F.D., R.Q.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8665-9532","authenticated-orcid":true,"given":"Raymond Q.","family":"Migrino","sequence":"additional","affiliation":[{"name":"Phoenix Veterans Affairs Healthcare System, AZ (S.A., S.T., N.K., D.R.G., J.L., R.Q.M.)."},{"name":"University of Arizona College of Medicine-Phoenix (D.R.G., T.V., J.L., A.F.D., R.Q.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(16)00114-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.109.552554"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.116.003318"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.119.014810"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.5607\/en.2019.28.2.216"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1694"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180822"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-015-0400-3"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/2040-7378-6-10"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144219"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD000094"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/0963689718822782"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/STROKEAHA.121.037120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T13:26:42Z","timestamp":1770384402000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/STROKEAHA.121.037120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1161\/STROKEAHA.121.037120"],"URL":"https:\/\/doi.org\/10.1161\/strokeaha.121.037120","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2]]},"assertion":[{"value":"2021-08-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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