{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T12:03:21Z","timestamp":1788955401370,"version":"build-2803163510"},"reference-count":12,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The immune response to acute cerebral ischemia is a major factor in stroke pathobiology. Circadian biology modulates some aspects of immune response. The goal of this study is to compare key parameters of immune response during the active\/awake phase versus inactive\/sleep phase in a mouse model of transient focal cerebral ischemia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Mice were housed in normal or reversed light cycle rooms for 3 weeks, and then they were blindly subjected to transient focal cerebral ischemia. Flow cytometry was used to examine immune responses in blood, spleen, and brain at 3 days after ischemic onset.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>In blood, there were higher levels of circulating T cells in mice subjected to focal ischemia during zeitgeber time (ZT)1\u20133 (inactive or sleep phase) versus ZT13\u201315 mice (active or awake phase). In the spleen, organ weight and immune cell numbers were lower in ZT1\u20133 versus ZT13\u201315 mice. Consistent with these results, there was an increased infiltration of activated T cells into brain at ZT1\u20133 compared with ZT13\u201315.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>This proof-of-concept study indicates that there are significant diurnal effects on the immune response after focal cerebral ischemia in mice. Hence, therapeutic strategies focused on immune targets should be reassessed to account for the effects of diurnal rhythms and circadian biology in nocturnal rodent models of stroke.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/strokeaha.122.040547","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T05:01:20Z","timestamp":1667365280000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":28,"title":["Diurnal Differences in Immune Response in Brain, Blood and Spleen After Focal Cerebral Ischemia in Mice"],"prefix":"10.1161","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8287-2747","authenticated-orcid":true,"given":"Elga","family":"Esposito","sequence":"first","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston (E.E., F.Z., J.H.P., E.T.M., W.L., E.H.L.)."},{"name":"Consortium International pour la Recherche Circadienne sur l\u2019AVC (CIRCA) (E.E., F.Z., J.H.P., E.T.M., W.L., I.L., M.A.M., E.H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1213-8993","authenticated-orcid":true,"given":"Fang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston (E.E., F.Z., J.H.P., E.T.M., W.L., E.H.L.)."},{"name":"Consortium International pour la Recherche Circadienne sur l\u2019AVC (CIRCA) (E.E., F.Z., J.H.P., E.T.M., W.L., I.L., M.A.M., E.H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4714-930X","authenticated-orcid":true,"given":"Ji-Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston (E.E., F.Z., J.H.P., E.T.M., W.L., E.H.L.)."},{"name":"Consortium International pour la Recherche Circadienne sur l\u2019AVC (CIRCA) (E.E., F.Z., J.H.P., E.T.M., W.L., I.L., M.A.M., E.H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7680-0923","authenticated-orcid":true,"given":"Emiri T.","family":"Mandeville","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston (E.E., F.Z., J.H.P., E.T.M., W.L., E.H.L.)."},{"name":"Consortium International pour la Recherche Circadienne sur l\u2019AVC (CIRCA) (E.E., F.Z., J.H.P., E.T.M., W.L., I.L., M.A.M., E.H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6507-392X","authenticated-orcid":true,"given":"Wenlu","family":"Li","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston (E.E., F.Z., J.H.P., E.T.M., W.L., E.H.L.)."},{"name":"Consortium International pour la Recherche Circadienne sur l\u2019AVC (CIRCA) (E.E., F.Z., J.H.P., E.T.M., W.L., I.L., M.A.M., E.H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Isabel","family":"Cuartero","sequence":"additional","affiliation":[{"name":"Neurovascular Pathophysiology Group, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain (M.I.C., M.A.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6028-7379","authenticated-orcid":true,"given":"Ignacio","family":"Lizasoa\u00edn","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n Neurovascular, Department of Pharmacology, Faculty of Medicine, Universidad Complutense and Instituto de Investigaci\u00f3n Hospital 12 de Octubre (i+12), Madrid, Spain (I.L.)."},{"name":"Consortium International pour la Recherche Circadienne sur l\u2019AVC (CIRCA) (E.E., F.Z., J.H.P., E.T.M., W.L., I.L., M.A.M., E.H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1010-8237","authenticated-orcid":true,"given":"Mar\u00eda A.","family":"Moro","sequence":"additional","affiliation":[{"name":"Neurovascular Pathophysiology Group, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain (M.I.C., M.A.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9327-2915","authenticated-orcid":true,"given":"Eng H.","family":"Lo","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston (E.E., F.Z., J.H.P., E.T.M., W.L., E.H.L.)."},{"name":"Consortium International pour la Recherche Circadienne sur l\u2019AVC (CIRCA) (E.E., F.Z., J.H.P., E.T.M., W.L., I.L., M.A.M., E.H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.11.001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2348-z"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.120.031742"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20978711"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.024950"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X18795789"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-018-0008-4"},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"353\u2013368","DOI":"10.1038\/s42003-019-0595-z","article-title":"SR9009 administered for one day after myocardial ischemia-reperfusion prevents heart failure in mice by targeting the cardiac inflammasome.","volume":"2","author":"Reitz CJ","year":"2019","unstructured":"Reitz, CJ, Alibhai, FJ, Khatua, TN, Rasouli, M, Bridle, BW, Burris, TP, Martino, TS. SR9009 administered for one day after myocardial ischemia-reperfusion prevents heart failure in mice by targeting the cardiac inflammasome. Commun Biol. 2019;2:353\u2013368.","journal-title":"Commun Biol"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1239-9"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.120.017759"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.120.029847"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178782"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/STROKEAHA.122.040547","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T10:46:54Z","timestamp":1715597214000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/STROKEAHA.122.040547"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":12,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1161\/STROKEAHA.122.040547"],"URL":"https:\/\/doi.org\/10.1161\/strokeaha.122.040547","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"2022-07-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}