{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T10:10:54Z","timestamp":1781777454799,"version":"3.54.5"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:sec>\n            <jats:title>BACKGROUND:<\/jats:title>\n            <jats:p>Functional activation of the focal ischemic brain has been reported to improve outcomes by augmenting collateral blood flow. However, functional activation also increases metabolic demand and might thereby worsen outcomes. Indeed, preclinical and clinical reports have been conflicting. Here, we tested the effect of functional activation during acute ischemic stroke using distal middle cerebral artery occlusion in anesthetized mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>METHODS:<\/jats:title>\n            <jats:p>Using transgenic mice expressing channelrhodopsin-2 in neurons, we delivered functional activation using physiological levels of transcranial optogenetic stimulation of the moderately ischemic cortex (ie, penumbra), identified using real-time full-field laser speckle perfusion imaging during a 1-hour distal microvascular clip of the middle cerebral artery. Neuronal activation was confirmed using evoked field potentials, and infarct volumes were measured in tissue slices 48 hours later.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>RESULTS:<\/jats:title>\n            <jats:p>\n              Optogenetic stimulation of the penumbra was associated with more than 2-fold larger infarcts than stimulation of the contralateral homotopic region and the sham stimulation group (n=10, 7, and 9; 11.0\u00b15.6 versus 5.1\u00b14.3 versus 4.1\u00b13.7 mm\n              <jats:sup>3<\/jats:sup>\n              ;\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              =0.008, 1-way ANOVA). Identical stimulation in wild-type mice that do not express channelrhodopsin-2 did not have an effect. Optogenetic stimulation was associated with a small increase in penumbral perfusion that did not explain enlarged infarcts.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>CONCLUSIONS:<\/jats:title>\n            <jats:p>Our data suggest that increased neuronal activity during acute focal arterial occlusions can be detrimental, presumably due to increased metabolic demand, and may have implications for the clinical management of hyperacute stroke patients.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/strokeaha.124.048032","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T09:05:11Z","timestamp":1725527111000},"page":"2502-2509","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":8,"title":["Optogenetic Functional Activation Is Detrimental During Acute Ischemic Stroke in Mice"],"prefix":"10.1161","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7666-2620","authenticated-orcid":true,"given":"Kazutaka","family":"Sugimoto","sequence":"first","affiliation":[{"name":"Department of Radiology (K.S., P.F., T.T., T.Q., C.A.), Massachusetts General Hospital, Harvard Medical School, Boston."},{"name":"Department of Neurosurgery, Yamaguchi University School of Medicine, Japan (K.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7149-5851","authenticated-orcid":true,"given":"David Y.","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Neurology (D.Y.C., C.A.), Massachusetts General Hospital, Harvard Medical School, Boston."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7673-8552","authenticated-orcid":true,"given":"Paul","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Radiology (K.S., P.F., T.T., T.Q., C.A.), Massachusetts General Hospital, Harvard Medical School, Boston."},{"name":"Department of Neurology with Experimental Neurology, Charit\u00e9-Universit\u00e4tsmedizin Berlin and Berlin Institute of Health at Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, BIH Biomedical Innovation Academy, Berlin Institute of Health Charit\u00e9 Junior Clinician Scientist Program, Germany (P.F.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2605-7200","authenticated-orcid":true,"given":"Tsubasa","family":"Takizawa","sequence":"additional","affiliation":[{"name":"Department of Radiology (K.S., P.F., T.T., T.Q., C.A.), Massachusetts General Hospital, Harvard Medical School, Boston."},{"name":"Department of Neurology, Keio University School of Medicine, Tokyo, Japan (T.T.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Radiology (K.S., P.F., T.T., T.Q., C.A.), Massachusetts General Hospital, Harvard Medical School, Boston."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4154-152X","authenticated-orcid":true,"given":"Mohammad A.","family":"Yaseen","sequence":"additional","affiliation":[{"name":"Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown (M.A.Y., S.S.)."},{"name":"Bioengineering Department, Northeastern University, Boston, MA (M.A.Y.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6318-1193","authenticated-orcid":true,"given":"Sava","family":"Sakad\u017ei\u0107","sequence":"additional","affiliation":[{"name":"Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown (M.A.Y., S.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3644-6042","authenticated-orcid":true,"given":"Cenk","family":"Ayata","sequence":"additional","affiliation":[{"name":"Department of Radiology (K.S., P.F., T.T., T.Q., C.A.), Massachusetts General Hospital, Harvard Medical School, Boston."},{"name":"Department of Neurology (D.Y.C., C.A.), Massachusetts General Hospital, Harvard Medical School, 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