{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T01:36:40Z","timestamp":1774661800352,"version":"3.50.1"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:p><jats:italic>Abstract<\/jats:italic>The vasodilator effect of \u03b2-adrenergic agonists has traditionally been ascribed solely to a direct effect on vascular smooth muscle. Experimental studies, however, have suggested a role of endothelium-derived nitric oxide (NO) in \u03b2-adrenergic\u2013mediated vasodilation. The purpose of this investigation was to determine whether NO contributes to the vasodilator effect of \u03b2-adrenergic stimulation in humans. We analyzed the forearm blood flow response to increasing doses of isoproterenol (50, 100, and 200 ng\/min), a \u03b2-adrenoceptor agonist, during the concomitant infusion of saline or<jats:italic>N<\/jats:italic><jats:sup>G<\/jats:sup>-monomethyl-<jats:sc>l<\/jats:sc>-arginine (L-NMMA; 4 \u03bcmol\/min), a blocker of NO synthesis, in 23 normal subjects (9 men and 14 women, aged 48\u00b17 years). The effect of L-NMMA was also assessed during infusion of sodium nitroprusside (0.8, 1.6, and 3.2 \u03bcg\/min), an exogenous NO donor. Drugs were infused into the brachial artery, and forearm blood flow was measured by plethysmography. The vasodilator effect of isoproterenol was significantly blunted during the administration of L-NMMA compared with saline (maximum flow, 7.7\u00b14 versus 11.2\u00b15 mL \u00b7 min<jats:sup>\u22121<\/jats:sup>\u00b7 dL<jats:sup>\u22121<\/jats:sup>, respectively;<jats:italic>P<\/jats:italic>&lt;.001). In contrast, the vasodilator response to sodium nitroprusside was not significantly affected by the infusion of L-NMMA (maximum flow, 8.8\u00b13.7 mL \u00b7 min<jats:sup>\u22121<\/jats:sup>\u00b7 dL<jats:sup>\u22121<\/jats:sup>during L-NMMA versus 8.9\u00b13.2 mL \u00b7 min<jats:sup>\u22121<\/jats:sup>\u00b7 dL<jats:sup>\u22121<\/jats:sup>during saline;<jats:italic>P<\/jats:italic>=.25). These findings indicate that NO inhibition blunts the vasodilator effect of \u03b2-adrenergic agonists in the human forearm and suggest that an abnormal response to adrenergic stimulation may occur in conditions associated with impaired NO activity.<\/jats:p>","DOI":"10.1161\/01.hyp.30.4.918","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:53:46Z","timestamp":1339462426000},"page":"918-921","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":65,"title":["Decreased Vasodilator Response to Isoproterenol During Nitric Oxide Inhibition in Humans"],"prefix":"10.1161","volume":"30","author":[{"given":"Carmine","family":"Cardillo","sequence":"first","affiliation":[{"name":"From the Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Crescence M.","family":"Kilcoyne","sequence":"additional","affiliation":[{"name":"From the Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arshed A.","family":"Quyyumi","sequence":"additional","affiliation":[{"name":"From the Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Richard O.","family":"Cannon","sequence":"additional","affiliation":[{"name":"From the Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio A.","family":"Panza","sequence":"additional","affiliation":[{"name":"From the Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_1_1_2","unstructured":"Kukovetz WR Poch C Holtzmann S. 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