{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:43:53Z","timestamp":1773809033186,"version":"3.50.1"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Objective\u2014<\/jats:italic>\n            <\/jats:bold>\n            6-Mercaptopurine (6-MP), the active metabolite of the immunosuppressive prodrug azathioprine, is commonly used in autoimmune diseases and transplant recipients, who are at high risk for cardiovascular disease. Here, we aimed to gain knowledge on the action of 6-MP in atherosclerosis, with a focus on monocytes and macrophages.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            We demonstrate that 6-MP induces apoptosis of THP-1 monocytes, involving decreased expression of the intrinsic antiapoptotic factors B-cell CLL\/Lymphoma-2 (Bcl-2) and Bcl2-like 1 (Bcl-x\n            <jats:sub>L<\/jats:sub>\n            ). In addition, we show that 6-MP decreases expression of the monocyte adhesion molecules platelet endothelial adhesion molecule-1 (PECAM-1) and very late antigen-4 (VLA-4) and inhibits monocyte adhesion. Screening of a panel of cytokines relevant to atherosclerosis revealed that 6-MP robustly inhibits monocyte chemoattractant chemokine-1 (MCP-1) expression in macrophages stimulated with lipopolysaccharide (LPS). Finally, local delivery of 6-MP to the vessel wall, using a drug-eluting cuff, attenuates atherosclerosis in hypercholesterolemic apolipoprotein E*3-Leiden transgenic mice (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). In line with our in vitro data, this inhibition of atherosclerosis by 6-MP was accompanied with decreased lesion monocyte chemoattractant chemokine-1 levels, enhanced vascular apoptosis, and reduced macrophage content.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusion\u2014<\/jats:italic>\n            <\/jats:bold>\n            We report novel, previously unrecognized atheroprotective actions of 6-MP in cultured monocytes\/macrophages and in a mouse model of atherosclerosis, providing further insight into the effect of the immunosuppressive drug azathioprine in atherosclerosis.\n          <\/jats:p>","DOI":"10.1161\/atvbaha.110.205674","type":"journal-article","created":{"date-parts":[[2010,4,23]],"date-time":"2010-04-23T02:40:00Z","timestamp":1271990400000},"page":"1591-1597","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":32,"title":["6-Mercaptopurine Inhibits Atherosclerosis in Apolipoprotein E*3-Leiden Transgenic Mice Through Atheroprotective Actions on Monocytes and Macrophages"],"prefix":"10.1161","volume":"30","author":[{"given":"Thijs W.H.","family":"Pols","sequence":"first","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Peter I.","family":"Bonta","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Nuno M.M.","family":"Pires","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Iker","family":"Otermin","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Mariska","family":"Vos","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Margreet R.","family":"de Vries","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Marco","family":"van Eijk","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Jeroen","family":"Roelofsen","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Louis M.","family":"Havekes","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Paul H.A.","family":"Quax","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Andre\u0301 B.P.","family":"van Kuilenburg","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Vivian","family":"de Waard","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Hans","family":"Pannekoek","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]},{"given":"Carlie J.M.","family":"de Vries","sequence":"additional","affiliation":[{"name":"From Departments of Medical Biochemistry (T.W.H.P., P.I.B., I.O., M.V., M.v.E., V.d.W., H.P., C.J.M.d.V.) and Genetic and Metabolic Diseases (J.R., A.B.P.v.K.), Academic Medical Center, Amsterdam, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine (N.M.M.P., M.R.d.V., P.H.A.Q.) and Department of Surgery (M.R.d.V., L.M.H., P.H.A.Q.), Leiden University Medical Center, Leiden, the Netherlands. Current address (N.M.M.P.): Department of Research and Development, BIAL, S. Mamede do..."}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-3565(04)00344-1"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2174\/138955708785132756"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.070968"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35025203"},{"key":"e_1_3_3_5_2","first-page":"RA247","volume":"10","year":"2004","unstructured":"Cara CJ, Pena AS, Sans M, Rodrigo L, Guerrero-Esteo M, Hinojosa J, Garcia-Paredes J, Guijarro LG Reviewing the mechanism of action of thiopurine drugs: towards a new paradigm in clinical practice. 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