{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:38:37Z","timestamp":1793039917921,"version":"4.5.0"},"reference-count":18,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2019,6,15]]},"abstract":"<jats:p>Introduction: Macroprolactinaemia is a well-known analytical problem in diagnostics of hyperprolactinaemia usually detected with polyethylene\nglycol (PEG) precipitation method. Since there is no harmonization in macroprolactin detection and reporting results, this study proposes and evaluates the usefulness of in-house developed algorithm. The aims were to determine the most suitable way of reporting results after PEG treatment and the possibilities of rationalizing the precipitation procedure.\nMaterials and methods: This is a retrospective study based on extracted data for 1136 patients. Prolactin concentrations were measured before\nand after PEG precipitation on Roche cobas e601. Macroprolactinaemia was defined by percentage recovery and post-PEG prolactin concentrations.\nResults: Prevalence of macroprolactinaemia using recovery criteria of \u2264 40%, \u2264 60%, and post-PEG prolactin concentrations was 3.3%, 8.8% and 7.8%, respectively. Raising the cut-off value from the upper limit of the manufacturer\u2019s reference interval to 32.9 \u03bcg\/L does not drastically change detected macroprolactinaemia with recovery criteria. Post-PEG prolactin concentrations showed more than half of the patients with macroprolactinaemia would be overlooked. Regardless of the criteria, a cut-off of 47.0 \u03bcg\/L would miss most of the macroprolactinaemic patients. Repeated recovery measurements of follow-up patients showed there is a significant difference with mean absolute bias of 9%.\nConclusions: Post-PEG prolactin concentration with corresponding reference interval is the most suitable way of reporting results. All samples with prolactin concentration above the upper limit of the manufacturer\u2019s reference interval should be submitted to PEG precipitation. Follow-up period could be prolonged since the difference between the recoveries of repeated measurements is not clinically significant.<\/jats:p>","DOI":"10.11613\/bm.2019.020706","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T07:55:02Z","timestamp":1560239702000},"page":"346-351","source":"Crossref","is-referenced-by-count":18,"title":["Optimizing laboratory defined macroprolactin algorithm"],"prefix":"10.11613","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1760-2852","authenticated-orcid":false,"given":"Milica","family":"\u0160ostari\u0107","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0795-3547","authenticated-orcid":false,"given":"Adriana","family":"Bokuli\u0107","sequence":"additional","affiliation":[{"name":"Laboratory of Endocrinology, Clinic of Oncology and Nuclear Medicine, Sestre Milosrdnice University Hospital Center, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1735-1865","authenticated-orcid":false,"given":"Domagoj","family":"Marijan\u010devi\u0107","sequence":"additional","affiliation":[{"name":"Laboratory of Endocrinology, Clinic of Oncology and Nuclear Medicine, Sestre Milosrdnice University Hospital Center, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Zec","sequence":"additional","affiliation":[{"name":"Laboratory of Endocrinology, Clinic of Oncology and Nuclear Medicine, Sestre Milosrdnice University Hospital Center, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4885","published-online":{"date-parts":[[2019,4,14]]},"reference":[{"key":"key20190611075459_r1","first-page":"161","article-title":"Macroprolactin; 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Croatian Society of Medical Biochemistry and Laboratory Medicine: national recommendations for venous blood sampling.","volume":"23","author":"Nikolac","year":"2013","journal-title":"Biochem Med (Zagreb)"},{"key":"key20190611075459_r16","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1373\/jalm.2016.022632","article-title":"Tracking Macroprolactin: Use of an Optimized Polyethylene Glycol Precipitation Method More Compatible with the Requirements and Processes of Automated Core Laboratories.","volume":"1","author":"Favresse","year":"2017","journal-title":"J Appl Lab Med"},{"key":"key20190611075459_r17","doi-asserted-by":"crossref","first-page":"3268","DOI":"10.1210\/jc.2010-0114","article-title":"Ten-year clinical follow-up of a cohort of 51 patients with macroprolactinemia establishes it as a benign variant.","volume":"95","author":"Wallace","year":"2010","journal-title":"J Clin Endocrinol Metab"},{"key":"key20190611075459_r18","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1530\/EJE-11-1007","article-title":"The natural history of macroprolactinaemia.","volume":"166","author":"Hattori","year":"2012","journal-title":"Eur J Endocrinol"}],"container-title":["Biochemia medica"],"original-title":[],"link":[{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/29\/2\/10.11613\/BM.2019.020706\/fullArticle","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T05:02:14Z","timestamp":1563426134000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/29\/2\/10.11613\/BM.2019.020706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,14]]},"references-count":18,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,4,14]]},"published-print":{"date-parts":[[2019,6,15]]}},"URL":"https:\/\/doi.org\/10.11613\/bm.2019.020706","relation":{},"ISSN":["1846-7482","1330-0962"],"issn-type":[{"value":"1846-7482","type":"electronic"},{"value":"1330-0962","type":"print"}],"subject":[],"published":{"date-parts":[[2019,4,14]]}}}