{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:44:45Z","timestamp":1765295085218},"reference-count":22,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2020,2,15]]},"abstract":"<jats:p>Rejection of the sample with repeated blood withdrawal is always an unwanted consequence of sample nonconformity and preanalytical errors,\nespecially in the most vulnerable population \u2013 children. Here is presented a case with unexpected abnormal coagulation test results in a 2-yearold\nchild with no previously documented coagulation disorder. Child is planned for tympanostomy tubes removal under the anaesthesia driven\nprocedure, and preoperative coagulation tests revealed prolonged prothrombin time, activated partial thromboplastin time and thrombin time,\nwith fibrinogen and antithrombin within reference intervals. From the anamnestic and clinical data, congenital coagulation disorder was excluded,\nand with further investigation, sample mismatch, clot presence and accidental ingestion of oral anticoagulant, heparin contamination or vitamin K\ndeficiency were excluded too. Due to suspected EDTA carryover during blood sampling another sample was taken the same day and all tests were\nperformed again. The results for all tests were within reference intervals confirming EDTA effect on falsely prolongation of the coagulation times in\nthe first sample. This case can serve as alert to avoid unnecessary loss in terms of blood withdrawal repetitions and discomfort of the patients and\ntheir relatives, tests repeating, prolonging medical procedures, and probably delaying diagnosis or proper medical treatment. It is the responsibility\nof the laboratory specialists to continuously educate laboratory staff and other phlebotomists on the correct blood collection as well as on its importance\nfor the patient\u2019s safety.<\/jats:p>","DOI":"10.11613\/bm.2020.011002","type":"journal-article","created":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T17:25:59Z","timestamp":1576257959000},"page":"158-163","source":"Crossref","is-referenced-by-count":1,"title":["Unexpected abnormal coagulation test results in a 2-year-old child"],"prefix":"10.11613","volume":"30","author":[{"given":"Patricija","family":"Bankovi\u0107 Radovanovi\u0107","sequence":"first","affiliation":[{"name":"Department of transfusion medicine, General Hospital Pula, Pula, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"\u017divkovi\u0107 Mikul\u010di\u0107","sequence":"additional","affiliation":[{"name":"Department of transfusion medicine, General Hospital Pula, Pula, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmina","family":"Simovi\u0107 Medica","sequence":"additional","affiliation":[{"name":"Department of transfusion medicine, General Hospital Pula, Pula, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4885","published-online":{"date-parts":[[2020,2,15]]},"reference":[{"key":"key20191213122553_r1","doi-asserted-by":"crossref","first-page":"288","DOI":"10.2478\/jomb-2014-0043","article-title":"Laboratory diagnostics and quality of blood collection.","volume":"34","author":"Lima-Oliveira","year":"2015","journal-title":"J Med Biochem"},{"key":"key20191213122553_r2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/978-1-4939-7196-1_2","article-title":"Preanalytical issues in hemostasis and thrombosis testing.","volume":"1646","author":"Lippi","year":"2017","journal-title":"Methods Mol Biol"},{"key":"key20191213122553_r3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1309\/LM749BQETKYPYPVM","article-title":"Pre-analytical variables in coagulation testing associated with diagnostic errors in hemostasis.","volume":"43","author":"Favaloro","year":"2012","journal-title":"Lab Med"},{"key":"key20191213122553_r4","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1055\/s-0032-1315961","article-title":"Lima-Oliveira, Guidi GC, Facaloro EJ. Quality standards for sample collection in coagulation testing.","volume":"38","author":"Lippi","year":"2012","journal-title":"Semin Thromb Hemost"},{"key":"key20191213122553_r5","doi-asserted-by":"crossref","first-page":"376","DOI":"10.11613\/BM.2014.040","article-title":"Clinical biochemistry laboratory rejection rates due to various types of preanalytical errors.","volume":"24","author":"Atay","year":"2014","journal-title":"Biochem Med (Zagreb)"},{"key":"key20191213122553_r6","unstructured":"Red Cross Blood Services. Blood types explained. Available at: https:\/\/www.redcrossblood.org\/donate-blood\/blood-types.html. Accessed June 6th, 2019."},{"key":"key20191213122553_r7"},{"key":"key20191213122553_r8","unstructured":"Onen ME. Anticoagulant used in haematology: Introduction to blood coagulation. Available at https:\/\/academia.edu\/8297641. Accessed June 6th, 2019."},{"key":"key20191213122553_r9"},{"key":"key20191213122553_r10"},{"key":"key20191213122553_r11","doi-asserted-by":"crossref","first-page":"242","DOI":"10.11613\/BM.2013.031","article-title":"Croatian society of medical biochemistry and laboratory medicine: National recommendations for venous blood sampling.","volume":"23","author":"Nikolac","year":"2013","journal-title":"Biochem Med (Zagreb)"},{"key":"key20191213122553_r12","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1515\/cclm-2014-1053","article-title":"Compliance of blood sampling procedures with the CLSI H3-A6 guidelines: An observational study by the European federation of clinical chemistry and laboratory medicine (EFLM) working group for the preanalytical phase (WG-PRE).","volume":"53","author":"Simundic","year":"2015","journal-title":"Clin Chem Lab Med"},{"key":"key20191213122553_r13","doi-asserted-by":"crossref","first-page":"218","DOI":"10.11613\/BM.2013.026","article-title":"Incorrect order of draw could be mitigate the patient safety: a phlebotomy management case report.","volume":"23","author":"Lima-Oliveira","year":"2013","journal-title":"Biochem Med (Zagreb)"},{"key":"key20191213122553_r14","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1111\/ijlh.12301","article-title":"Sodium citrate blood contamination by K2-ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing.","volume":"37","author":"Lima-Oliveira","year":"2015","journal-title":"Int J Lab Hematol"},{"key":"key20191213122553_r15","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1136\/jclinpath-2011-200206","article-title":"Effect of order of draw of blood samples during phlebotomy on routine biochemistry results.","volume":"64","author":"Sulaiman","year":"2011","journal-title":"J Clin Pathol"},{"key":"key20191213122553_r16","doi-asserted-by":"crossref","DOI":"10.1515\/cclm-2013-0650","article-title":"The order of draw, myth or science.","volume":"51","author":"Cornes","year":"2013","journal-title":"Clin Chem Lab Med"},{"key":"key20191213122553_r17","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1515\/cclm-2013-0412","article-title":"The order of draw: myth or science?","volume":"51","author":"Salvagno","year":"2013","journal-title":"Clin Chem Lab Med"},{"key":"key20191213122553_r18","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/ijlh.12230","article-title":"The order of draw: much ado about nothing?","volume":"37","author":"Indevuyst","year":"2015","journal-title":"Int J Lab Hematol"},{"key":"key20191213122553_r19","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1080\/09674845.2012.12069141","article-title":"Incorrect order of draw of blood samples does not cause potassium EDTA sample contamination.","volume":"69","author":"Cornes","year":"2012","journal-title":"Br J Biomed Sci"},{"key":"key20191213122553_r20","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1515\/cclm-2016-0426","article-title":"Order of blood draw: Opinion Paper by the European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE).","volume":"55","author":"Cornes","year":"2017","journal-title":"Clin Chem Lab Med"},{"key":"key20191213122553_r21","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1515\/cclm-2014-0944","article-title":"Relevance of EDTA carryover during blood collection.","volume":"53","author":"Cadamuro","year":"2015","journal-title":"Clin Chem Lab Med"},{"key":"key20191213122553_r22","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/ijlh.12434","article-title":"Underestimation of plasma level of factor V coagulant activity and fibrinogen concentration together with prolonged prothrombin time, activated partial thromboplastin time and thrombin time can result from pre-analytical very low calcium level in citrated sample tube.","volume":"38","author":"d\u2019Audigier","year":"2016","journal-title":"Int J Lab Hematol"}],"container-title":["Biochemia medica"],"original-title":[],"link":[{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/30\/1\/10.11613\/BM.2020.011002\/fullArticle","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T13:43:19Z","timestamp":1581946999000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/30\/1\/10.11613\/BM.2020.011002"}},"subtitle":["A case report"],"short-title":[],"issued":{"date-parts":[[2020,2,15]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,2,15]]},"published-print":{"date-parts":[[2020,2,15]]}},"URL":"https:\/\/doi.org\/10.11613\/bm.2020.011002","relation":{},"ISSN":["1846-7482","1330-0962"],"issn-type":[{"value":"1846-7482","type":"electronic"},{"value":"1330-0962","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2,15]]}}}