{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:55:31Z","timestamp":1787018131189,"version":"build-2736575974"},"reference-count":31,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2021,2,15]]},"abstract":"<jats:p>Introduction: We investigated the interference of haemolysis on ethanol testing carried out with the Synchron assay kit using an AU680 autoanalyser\n(Beckman Coulter, Brea, USA).\nMaterials and methods: Two tubes of plasma samples were collected from 20 volunteers. Mechanical haemolysis was performed in one tube,\nand no other intervention was performed in the other tube. After centrifugation, haemolysed and non-haemolysed samples were diluted to obtain\nsamples with the desired free haemoglobin (Hb) values (0, 1, 2, 5, 10 g\/L). A portion of these samples was then separated, and ethanol was added to\nthe separated sample to obtain a concentration of 86.8 mmol\/L ethanol. After that, these samples were diluted with ethanol-free samples with the\nsame Hb concentration to obtain samples containing 43.4, 21.7, and 10.9 mmol\/L. Each group was divided into 20 equal parts, and an ethanol test\nwas carried out. The coefficient of variation (CV), bias, and total error (TE) values were calculated.\nResults: The TE values of haemolysis-free samples were approximately 2-5%, and the TE values of haemolysed samples were approximately 10-\n18%. The bias values of haemolysed samples ranged from nearly - 6.2 to - 15.7%.\nConclusions: Haemolysis led to negative interference in all samples. However, based on the 25% allowable total error value specified for ethanol in\nthe Clinical Laboratory Improvement Amendments (CLIA 88) criteria, the TE values did not exceed 25%. Consequently, ethanol concentration can be\nmeasured in samples containing free Hb up to 10 g\/L.<\/jats:p>","DOI":"10.11613\/bm.2021.010704","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T16:05:30Z","timestamp":1608307530000},"page":"85-92","source":"Crossref","is-referenced-by-count":6,"title":["Effect of haemolysis on an enzymatic measurement of ethanol"],"prefix":"10.11613","volume":"31","author":[{"given":"Abdulkadir","family":"\u00c7at","sequence":"first","affiliation":[{"name":"Istanbul Gaziosmanpasa Training and Research Hospital, Medical Biochemistry, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamil Taha","family":"U\u00e7ar","sequence":"additional","affiliation":[{"name":"Istanbul Gaziosmanpasa Training and Research Hospital, Medical Biochemistry, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alper","family":"G\u00fcm\u00fc\u015f","sequence":"additional","affiliation":[{"name":"Istanbul Gaziosmanpasa Training and Research Hospital, Medical Biochemistry, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4885","published-online":{"date-parts":[[2021,2,16]]},"reference":[{"key":"key20201218160517_r1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.ejim.2007.06.033","article-title":"Acute alcohol intoxication.","volume":"19","author":"Vonghia","year":"2008","journal-title":"Eur J Intern Med"},{"key":"key20201218160517_r2","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.ajem.2017.01.009","article-title":"Developing a standardized measurement of alcohol intoxication.","volume":"35","author":"Benoit","year":"2017","journal-title":"Am J Emerg Med"},{"key":"key20201218160517_r3","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1515\/CCLM.2008.170","article-title":"Haemolysis: An overview of the leading cause of unsuitable specimens in clinical laboratories.","volume":"46","author":"Lippi","year":"2008","journal-title":"Clin Chem Lab Med"},{"key":"key20201218160517_r4","doi-asserted-by":"crossref","first-page":"229","DOI":"10.5858\/arpa.2016-0400-OA","article-title":"Preanalytic factors associated with hemolysis in emergency department blood samples.","volume":"142","author":"Phelan","year":"2018","journal-title":"Arch Pathol Lab Med"},{"key":"key20201218160517_r5","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1093\/jat\/bkv068","article-title":"The impact of hemolysis on the accuracy of ethanol determinations.","volume":"39","author":"Tiscione","year":"2015","journal-title":"J Anal Toxicol"},{"key":"key20201218160517_r6","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1093\/jat\/19.3.192","article-title":"Serum-ethanol determination: Comparison of lactate and lactate dehydrogenase interference in three enzymatic assays.","volume":"19","author":"Nine","year":"1995","journal-title":"J Anal Toxicol"},{"key":"key20201218160517_r7","first-page":"S43","article-title":"Interference testing.","volume":"29","author":"Dimeski","year":"2008","journal-title":"Clin Biochem Rev"},{"key":"key20201218160517_r8","first-page":"399","article-title":"Study of forensic and clinical source hemoglobin interference with the duPont aca ethanol method.","volume":"16","author":"Gadsden","year":"1986","journal-title":"Ann Clin Lab Sci"},{"key":"key20201218160517_r9","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/j.cca.2011.04.034","article-title":"Evaluation of the interference of hemoglobin, bilirubin, and lipids on Roche Cobas 6000 assays.","volume":"412","author":"Ji","year":"2011","journal-title":"Clin Chim Acta"},{"key":"key20201218160517_r10","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.cca.2011.10.002","article-title":"The concentration of plasma ethanol measured with an enzymatic assay is decreased in hemolyzed specimens.","volume":"413","author":"Lippi","year":"2012","journal-title":"Clin Chim Acta"},{"key":"key20201218160517_r11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10408363.2019.1664391","article-title":"Managing hemolyzed samples in clinical laboratories.","volume":"57","author":"Simundic","year":"2020","journal-title":"Crit Rev Clin Lab Sci"},{"key":"key20201218160517_r12"},{"key":"key20201218160517_r13","unstructured":"CLIA Requirements for Analytical Quality. Available at: https:\/\/www.westgard.com\/clia.htm. Accessed at: November 22, 2019."},{"key":"key20201218160517_r14","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1093\/jat\/bkt054","article-title":"Scientific Working Group for Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology.","volume":"37","author":"Scientific Working Group for Forensic Toxicology","year":"2013","journal-title":"J Anal Toxicol"},{"key":"key20201218160517_r15","unstructured":"Interference and Recovery Experiments. Available at: https:\/\/www.westgard.com\/lesson27.htm. Accessed at: November 22, 2019."},{"key":"key20201218160517_r16","doi-asserted-by":"crossref","DOI":"10.1155\/2017\/5849593","article-title":"Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and in Vitro Study.","volume":"2017","author":"Alexandrovich","year":"2017","journal-title":"BioMed Res Int"},{"key":"key20201218160517_r17","first-page":"595","article-title":"Haemoglobin Interference in the Bichromatic Spectrophotometry of NAD(P)H at 340\/380 nm.","volume":"31","author":"da Fonseca-Wollheim","year":"1993","journal-title":"Eur J Clin Chem Clin Biochem"},{"key":"key20201218160517_r18"},{"key":"key20201218160517_r19","doi-asserted-by":"crossref","first-page":"317","DOI":"10.2478\/jomb-2018-0032","article-title":"Determining the need for repeat testing of blood ethanol concentration: Evaluation of the synchron blood ethyl alcohol assay kit.","volume":"38","author":"Ustunda\u011f","year":"2019","journal-title":"J Med Biochem"},{"key":"key20201218160517_r20","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1515\/cclm-2015-0067","article-title":"Defining analytical performance specifications: Consensus Statement from the 1st Strategic Conference of the European Federation of Clinical Chemistry and Laboratory Medicine.","volume":"53","author":"Sandberg","year":"2015","journal-title":"Clin Chem Lab Med"},{"key":"key20201218160517_r21","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1515\/cclm-2017-1104","article-title":"Practical recommendations for managing hemolyzed samples in clinical chemistry testing.","volume":"56","author":"Lippi","year":"2018","journal-title":"Clin Chem Lab Med"},{"key":"key20201218160517_r22"},{"issue":"1","key":"key20201218160517_r23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cll.2016.09.001","article-title":"Measuring Analytical Quality: Total Analytical Error Versus Measurement Uncertainty.","volume":"37","author":"Westgard","year":"2017","journal-title":"Clin Lab Med"},{"key":"key20201218160517_r24","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1515\/cclm-2018-0421","article-title":"Uncertainty in measurement and total error: Different roads to the same quality destination?","volume":"56","author":"Farrance","year":"2018","journal-title":"Clin Chem Lab Med"},{"key":"key20201218160517_r25","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.cca.2018.03.043","article-title":"Call for more transparency in manufacturers declarations on serum indices: On behalf of the Working Group for Preanalytical Phase (WG-PRE), European Federation of Clinical Chemistry and Laboratory Medicine (EFLM).","volume":"484","author":"von Meyer","year":"2018","journal-title":"Clin Chim Acta"},{"key":"key20201218160517_r26","article-title":"European survey on preanalytical sample handling - Part 2: Practices of European laboratories on monitoring and processing haemolytic, icteric and lipemic samples. 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