{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:48:05Z","timestamp":1765295285587},"reference-count":19,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2022,2,15]]},"abstract":"<jats:p>The aims of study were to assess: 1) performance specifications of Atellica 1500, 2) comparability of Atellica 1500 and Iris, 3) the accuracy of both analysers in their ability to detect bacteria.\nCarryover, linearity, precision, reproducibility, and limit of blank (LoB) verification were evaluated for erythrocyte and leukocyte counts. ICSH 2014 protocol was used for estimation of carryover, CLSI EP15-A3 for precision, and CLSI EP17 for LoB verification. Comparison for quantitative parameters was evaluated by Bland-Altman plot and Passing-Bablok regression. Qualitative parameters were evaluated by Weighted kappa analysis. Sixty-five urine samples were randomly selected and sent for urine culture which was used as reference method to determine the accuracy of bacteria detection by analysers.\nAnalytical specifications of Atellica 1500 were successfully verified. Total of 393 samples were used for qualitative comparison, while 269 for sediment urinalysis. Bland-Altman analysis showed statistically significant proportional bias for erythrocytes and leukocytes. Passing-Bablok analysis for leukocytes pointed to significant constant and minor proportional difference, while it was not performed for erythrocytes due to significant data deviation from linearity. Kappa analysis resulted in the strongest agreements for pH, ketones, glucose concentrations and leukocytes, while the poorest agreement for bacteria. The sensitivity and specificity of bacteria detection were: 91 (59-100)% and 76 (66-87)% for Atellica 1500 and 46 (17-77)% and 96 (87-100)% for Iris.\nThere are large differences between Atellica 1500 and Iris analysers, due to which they are not comparable and can not be used interchangeably. While there was no difference in specificity of bacteria detection, Iris analyser had greater sensitivity.<\/jats:p>","DOI":"10.11613\/bm.2022.010701","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T09:14:42Z","timestamp":1639991682000},"page":"24-32","source":"Crossref","is-referenced-by-count":1,"title":["Verification of Atellica 1500 and comparison with Iris urine analyser and urine culture"],"prefix":"10.11613","volume":"32","author":[{"given":"Helena","family":"\u010ci\u010dak","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Diagnostics, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danijela","family":"Bejuk","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Hospital Infections, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanja","family":"Radi\u0161i\u0107 Biljak","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Diagnostics, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana-Maria","family":"\u0160imundi\u0107","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Diagnostics, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Nikler","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Diagnostics, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4885","reference":[{"key":"key20211220041437_r1","first-page":"141","article-title":"The importance of microscopic examination of the urinary sediment.","volume":"48","author":"Szwed","year":"1982","journal-title":"Am J Med Technol"},{"key":"key20211220041437_r2","unstructured":"National Institution for Health and Care Excellence (NICE). Chronic kidney disease in adults: assessment and management; Clinical guideline. Available from: https:\/\/www.nice.org.uk\/guidance\/cg182. Accessed July 5th 2019."},{"key":"key20211220041437_r3","unstructured":"Public Health England. Diagnosis of urinary tract infections (UTIs). Quick reference guide for primary care: For consultation and local adaptation. Available from: www.gov.uk\/government\/consultations\/diagnosis-of-urinary-tract-infections-quick-reference-guide. Accessed July 5th 2019."},{"key":"key20211220041437_r4","unstructured":"The American Urological Association. Renal Mass and Localized Renal Cancer: AUA Guideline. Available from: https:\/\/www.auanet.org\/guidelines\/renal-cancer-renal-mass-and-localized-renal-cancer-guideline#x7068. Accessed July 5th 2019."},{"key":"key20211220041437_r5","first-page":"S49","article-title":"Automatization of urine analysis.","volume":"28","author":"Bilic-Zulle","year":"2018","journal-title":"Biochem Med (Zagreb)"},{"key":"key20211220041437_r6","first-page":"S91","article-title":"Experience in automated urinalysis in private laboratory\u2019s daily routine.","volume":"28","author":"Kozic","year":"2018","journal-title":"Biochem Med (Zagreb)"},{"key":"key20211220041437_r7","doi-asserted-by":"crossref","first-page":"276","DOI":"10.11613\/BM.2012.031","article-title":"Interrater reliability: the kappa statistic.","volume":"22","author":"McHugh","year":"2012","journal-title":"Biochem Med (Zagreb)"},{"key":"key20211220041437_r8","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/ijlh.12201","article-title":"ICHS guidelines for the evaluation of blood cell analysers including those used for differential leucocyte and reticulocyte counting.","volume":"36","author":"Briggs","year":"2014","journal-title":"Int J Lab Hematol."},{"key":"key20211220041437_r9","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1177\/000456327401100164","article-title":"A revised scheme for the evaluation of automatic instruments for use in clinical chemistry.","volume":"11","author":"Broughton","year":"1974","journal-title":"Ann Clin Biochem"},{"key":"key20211220041437_r10"},{"key":"key20211220041437_r11"},{"key":"key20211220041437_r12","doi-asserted-by":"crossref","DOI":"10.11613\/BM.2019.010708","article-title":"Analytical verification of 12 most commonly used urine dipsticks in Croatia: comparability, repetability and accuracy.","volume":"29","author":"Vuljani\u0107","year":"2019","journal-title":"Biochem Med (Zagreb)"},{"key":"key20211220041437_r13","first-page":"S189","article-title":"Analytical verification of 12 most commonly used urine dipsticks in Croatia: comparability, reproducibility and accuracy.","volume":"28","author":"Vuljanic","year":"2018","journal-title":"Biochem Med (Zagreb)"},{"key":"key20211220041437_r14","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3343\/alm.2019.39.1.15","article-title":"Progress in automated urinalysis.","volume":"39","author":"Oyaert","year":"2019","journal-title":"Ann Lab Med"},{"key":"key20211220041437_r15","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.plabm.2016.03.002","article-title":"The comparison of automated urine analysers with manual microscopic examination for urinalysis automated urine analysers and manual urinalysis.","volume":"5","author":"\u0130nce","year":"2016","journal-title":"Pract Lab Med"},{"key":"key20211220041437_r16","doi-asserted-by":"crossref","DOI":"10.11613\/BM.2018.020712","article-title":"Evaluation of the analytical performances of Cobas 6500 and Sysmex UN series automated urinalysis systems with manual microscopic particle counting.","volume":"28","author":"Bakan","year":"2018","journal-title":"Biochem Med (Zagreb)"},{"key":"key20211220041437_r17","doi-asserted-by":"crossref","DOI":"10.1002\/jcla.22249","article-title":"UriSed 3 and UX-2000 automated urine sediment analysers vs manual microscopic method: A comparative performance analysis.","volume":"32","author":"Laiwejpithaya","year":"2018","journal-title":"J Clin Lab Anal"},{"key":"key20211220041437_r18","first-page":"S156","article-title":"Significance of automation in standardization of qualitative urinalysis.","volume":"28","author":"Barbir","year":"2018","journal-title":"Biochem Med (Zagreb)"},{"key":"key20211220041437_r19","doi-asserted-by":"crossref","first-page":"e02030","DOI":"10.1128\/JCM.02030-17","article-title":"Use of automated urine microscopy analysis in clinical diagnosis of urinary tract infection: defining an optimal diagnostic score in an academic medical center population.","volume":"56","author":"Foudraine","year":"2018","journal-title":"J Clin Microbiol"}],"container-title":["Biochemia medica"],"original-title":[],"link":[{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/32\/1\/10.11613\/BM.2022.010701\/fullArticle","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T06:49:41Z","timestamp":1645512581000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/32\/1\/10.11613\/BM.2022.010701"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,15]]},"references-count":19,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,2,15]]},"published-print":{"date-parts":[[2022,2,15]]}},"URL":"https:\/\/doi.org\/10.11613\/bm.2022.010701","relation":{},"ISSN":["1846-7482","1330-0962"],"issn-type":[{"value":"1846-7482","type":"electronic"},{"value":"1330-0962","type":"print"}],"subject":[],"published":{"date-parts":[[2022,2,15]]}}}