{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:34:58Z","timestamp":1787243698976,"version":"build-2736575974"},"reference-count":20,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2022,10,15]]},"abstract":"<jats:p>IntroductionSystemic sclerosis (Ssc) is a multiorgan debilitating autoimmune disease that associates the triad: vascular involvement, tissue fibrosis and profound immune response alterations. Numerous previous studies focused on identification of candidate proteomic Ssc biomarkers using mass-spectrometry techniques and a large number of candidate Ssc biomarkers emerged. These biomarkers must firstly be confirmed in independent patient groups. The aim of the present study was to investigate the association of cytokeratin 17 (CK17), marginal zone B1 protein (MZB1) and leucine-rich \u03b12-glycoprotein-1 (LRG1) with clinical and biological Ssc characteristics.\nMaterial and methodsSerum CK17, MZB1 and LRG1 were assessed in samples of the available Ssc biobank comprising of samples from 53 Ssc patients and 26 matched age and gender controls.\nResultsCirculatory CK17, LRG1 and MZB1 concentrations were increased in Ssc patients. Cytokeratin 17 is independently associated with Ssc disease activity. Patients with pulmonary fibrosis expressed higher LRG1 and MZB1 concentrations. Serum MZB1 concentrations were also associated with extensive skin fibrosis.\nConclusionsSerum CK17, MZB1 and LRG1 were confirmed biomarkers for Ssc. LRG1 seems a good biomarker for pulmonary fibrosis, while MZB1 is a good biomarker for extensive skin fibrosis. CK17 proved to be independently associated with Ssc disease severity, higher CK17 values being protective for a more active disease.<\/jats:p>","DOI":"10.11613\/bm.2022.030707","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T02:28:02Z","timestamp":1663900082000},"page":"414-421","source":"Crossref","is-referenced-by-count":14,"title":["Circulatory cytokeratin 17, marginal zone B1 protein and leucine-rich \u03b12-glycoprotein-1 as biomarkers for disease severity and fibrosis in systemic sclerosis patients"],"prefix":"10.11613","volume":"32","author":[{"given":"Anca","family":"B\u0103l\u0103nescu","sequence":"first","affiliation":[{"name":"Pediatrics Department, University of Medicine and Pharmacy Carol Davila, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian","family":"B\u0103icu\u0219","sequence":"additional","affiliation":[{"name":"Internal Medicine Department, University of Medicine and Pharmacy Carol Davila, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"B\u0103l\u0103nescu","sequence":"additional","affiliation":[{"name":"Clinical Immunology Laboratory CDPC, Colentina Clinical Hospital, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"B\u0103l\u0103nescu","sequence":"additional","affiliation":[{"name":"Internal Medicine Department, University of Medicine and Pharmacy Carol Davila, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4885","published-online":{"date-parts":[[2022,9,29]]},"reference":[{"key":"key20220923022758_r1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.5114\/reum.2019.87619","article-title":"Systemic sclerosis - multidisciplinary disease: clinical features and 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