{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:39:27Z","timestamp":1778884767048,"version":"3.51.4"},"reference-count":28,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2023,2,15]]},"abstract":"<jats:p>IntroductionThe aim of research was to assess the melatonin concentrations in the early neonatal period as a predictor of adverse outcomes of late neonatal period in preterm infants and to estimate its optimal predictive cut-off values.\nMaterials and methodsA total of 115 preterm infants admitted to the neonatal intensive care unit were screened for eligibility, five did not meet the criteria, six parents declined the participation. So, a total of 104 preterm infants with gestational age 25-34 weeks were included in research. The concentration of melatonin in urine was determined by the Enzyme Immunoassay method (Human Melatonin Sulfate ELISA kit, Elabscience, China). The Mann-Whitney U-test and analysis of the receiver operating characteristic (ROC) curve were used in statistical analysis.\nResultsAnalysis of the ROC curves has revealed optimal cut-off values for urinary melatonin concentration to predict late outcomes. Melatonin concentration below 3.58 ng\/ml with sensitivity of 72% can predict development of retinopathy of prematurity (ROP) (AUC = 0.73; 95% confidence intervals (CI) 0.61-0.86). Good diagnostic accuracy (AUC = 0.80; 95% CI 0.67-0.93) has been shown for bronchopulmonary dysplasia (BPD). The optimal cut-off value for melatonin concentration in BPD prediction is 3.71 ng\/ml (sensitivity 80%, specificity 64%). Urinary melatonin concentration below 3.79 ng\/ml can be associated with late-onset sepsis (AUC = 0.76; 95% CI 0.64-0.87; sensitivity 72%; specificity 62%). There were no significant associations between melatonin concentration and necrotizing enterocolitis (P = 0.912).\nConclusionUrinary melatonin concentration below the certain cut-off values in the early neonatal period may serve as one of the predictors of adverse outcomes such as BPD, ROP, and late-onset sepsis in the late neonatal period in preterm infants.<\/jats:p>","DOI":"10.11613\/bm.2023.010706","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T12:48:18Z","timestamp":1671454098000},"source":"Crossref","is-referenced-by-count":3,"title":["The relationship of melatonin concentration in preterm infants and adverse outcomes in the late neonatal period"],"prefix":"10.11613","volume":"33","author":[{"given":"Kateryna","family":"Kozak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u041dalyna","family":"Pavlyshyn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iryna","family":"Sarapuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4885","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"key20221219074811_r1","doi-asserted-by":"crossref","first-page":"3027","DOI":"10.1016\/S0140-6736(16)31593-8","article-title":"Global, regional, and national causes of under-5 mortality in 2000-15: an updated systematic analysis with implications for the Sustainable Development Goals.","volume":"388","author":"Liu","year":"2016","journal-title":"Lancet"},{"key":"key20221219074811_r2","article-title":"at al. 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Preterm birth factsheet. 2018. Available from: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/preterm-birth. Accessed May 9th 2022."},{"key":"key20221219074811_r15","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3390\/ph13070145","article-title":"Oxidative stress in preterm infants: overview of current evidence and future prospects.","volume":"13","author":"Falsaperla","year":"2020","journal-title":"Pharmaceuticals."},{"key":"key20221219074811_r16","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1111\/j.1600-079X.2005.00251.x","article-title":"Correlation among cytokines, bronchopulmonary dysplasia and modality of ventilation in preterm newborns: improvement with melatonin treatment.","volume":"39","author":"Gitto","year":"2005","journal-title":"J Pineal Res"},{"key":"key20221219074811_r17","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jpeds.2017.08.005","article-title":"Can we prevent bronchopulmonary dysplasia?","volume":"189","author":"Aschner","year":"2017","journal-title":"J Pediatr"},{"key":"key20221219074811_r18"},{"key":"key20221219074811_r19","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1055\/s-2004-828610","article-title":"Oxidative and inflammatory parameters in respiratory distress syndrome of preterm newborns: beneficial effects of melatonin.","volume":"21","author":"Gitto","year":"2004","journal-title":"Am J Perinatol"},{"key":"key20221219074811_r20","doi-asserted-by":"crossref","first-page":"148","DOI":"10.33069\/cim.2020.0024","article-title":"Role of Melatonin in Preterm Birth.","volume":"2","author":"Khan","year":"2020","journal-title":"Chronobiol Med."},{"key":"key20221219074811_r21","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1203\/PDR.0b013e318193f165","article-title":"Bronchopulmonary dysplasia in a rat model induced by intra-amniotic inflammation and postnatal hyperoxia: morphometric aspects.","volume":"65","author":"Choi","year":"2009","journal-title":"Pediatr Res"},{"key":"key20221219074811_r22","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.survophthal.2018.04.002","article-title":"Retinopathy of prematurity: a review of risk factors and their clinical significance.","volume":"63","author":"Kim","year":"2018","journal-title":"Surv Ophthalmol"},{"key":"key20221219074811_r23","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1159\/000433565","article-title":"Evaluation of Oxygen Supplementation Status as a Risk Factor Associated with the Development of Severe Retinopathy of Prematurity.","volume":"234","author":"Enomoto","year":"2015","journal-title":"Ophthalmologica"},{"key":"key20221219074811_r24","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.preteyeres.2012.07.003","article-title":"Neuromodulatory role of melatonin in retinal information processing.","volume":"32","author":"Huang","year":"2013","journal-title":"Prog Retin Eye Res"},{"key":"key20221219074811_r25","first-page":"378","article-title":"Role of non-enzymatic antioxidants in neonatal septicemia.","volume":"2","author":"Narad","year":"2013","journal-title":"Int J Cur Tr Res."},{"key":"key20221219074811_r26","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1203\/00006450-200112000-00021","article-title":"Effects of melatonin treatment in septic newborns.","volume":"50","author":"Gitto","year":"2001","journal-title":"Pediatr Res"},{"key":"key20221219074811_r27","doi-asserted-by":"crossref","first-page":"7730","DOI":"10.7150\/thno.45862","article-title":"Melatonin ameliorates necrotizing enterocolitis by preventing Th17\/Treg imbalance through activation of the AMPK\/SIRT1 pathway.","volume":"10","author":"Ma","year":"2020","journal-title":"Theranostics"},{"key":"key20221219074811_r28","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/6920577","article-title":"Melatonin Alleviates Neonatal Necrotizing Enterocolitis by Repressing the Activation of the NLRP3 Inflammasome.","volume":"2022","author":"Xiong","year":"2022","journal-title":"Gastroenterol Res Pract"}],"container-title":["Biochemia medica"],"original-title":[],"link":[{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/33\/1\/10.11613\/BM.2023.010706\/fullArticle","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T11:07:36Z","timestamp":1672225656000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/33\/1\/10.11613\/BM.2023.010706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,15]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,2,15]]}},"URL":"https:\/\/doi.org\/10.11613\/bm.2023.010706","relation":{},"ISSN":["1846-7482","1330-0962"],"issn-type":[{"value":"1846-7482","type":"electronic"},{"value":"1330-0962","type":"print"}],"subject":[],"published":{"date-parts":[[2022,12,15]]}}}