{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T00:17:05Z","timestamp":1718237825003},"reference-count":30,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2024,2,15]]},"abstract":"<jats:p>IntroductionParaoxonase 1 (PON1) is the enzyme that removes carcinogenic radicals from lipids. The aim of the study was to investigate the differences in PON1 activity and oxidation stress parameters between patients with cervical intraepithelial neoplasia (CIN) and healthy controls. Materials and methodsThe study included 65 women with CIN and 109 healthy women. Lipid parameters were determined on Cobas Integra 400 plus (Roche, Mannheim, Germany). Tiols and reduced glutathione (GSH) were determined spectrophotometric using Eliman reagent. Activity of PON1 was assessed with two substrates, paraoxon and phenylacetate by spectrophotometric method. Malondialdehyde (MDA) was determined by high performance liquid chromatography (Shimadzu Corporation, Kyoto, Japan). Mann-Whitney-test, t-test, \u03c72-test, correlation and logistic regression was used in statistical analysis. P &amp;lt; 0.05 was considered statistically significant. ResultsThe basal (P = 0.929) and NaCl-stimulated (P = 0.985) PON1 activity and activities standardised on the concentration of high-density lipoprotein (HDL; P = 0.076; P = 0.065, respectively) and apolipoprotein AI (apo AI; P = 0.444; P = 0.499, respectively) as well as PON1 phenotypes (P = 0.842) did not differ significantly between the groups. The PON1 arylesterase activity (53\u00b119 kU\/L vs. 77\u00b117 kU\/L; P &amp;lt; 0.001) and HDL-standardized activity (37 (28-44) kU\/mmol vs. 43 (37-50) kU\/mmol; P &amp;lt; 0.001) and apoAI (29\u00b111 kU\/g vs. 44\u00b111 kU\/g; P &amp;lt; 0.001) was significantly reduced in the CIN group. The concentration of the thiol groups was similar (P = 0.519), of MDA was lower (0.39 (0.27-0.55) \u00b5mol\/L vs. 0.76 (0.57-1.15) \u00b5mol\/L; P &amp;lt; 0.001) and of GSH was higher (112.0 (66.0-129.6) \u00b5g\/mL vs. 53.4 (34.8-134.4) \u00b5g\/mL; P &amp;lt; 0.001) in the CIN group. ConclusionReduced PON1 arylesterase activity, lower MDA and higher GSH concentration were observed in CIN patients.<\/jats:p>","DOI":"10.11613\/bm.2024.010701","type":"journal-article","created":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T13:02:13Z","timestamp":1702990933000},"page":"72-79","source":"Crossref","is-referenced-by-count":0,"title":["Paraoxonase and arylesterase activity of paraoxonase 1 and oxidative stress parameters in cervical intraepithelial neoplasia"],"prefix":"10.11613","volume":"34","author":[{"given":"Marija Grdi\u0107","family":"Rajkovi\u0107","sequence":"first","affiliation":[{"name":"Department of Medical biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dubravka","family":"Ra\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Unit of Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Stojanovi\u0107","sequence":"additional","affiliation":[{"name":"Clinic of Gynecology and Obstetrics, Sestre milosrdnice University Hospital Center, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Tur\u010di\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomislav","family":"Mileti\u0107","sequence":"additional","affiliation":[{"name":"Polyclinic Aviva, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea Hulina","family":"Toma\u0161kovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Medical biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanja","family":"Ka\u010dkov Masla\u0107","sequence":"additional","affiliation":[{"name":"Medical biochemistry laboratory, Polyclinic Bonifarm, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"\u0106elap","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dra\u017ean","family":"Butorac","sequence":"additional","affiliation":[{"name":"Clinic of Gynecology and Obstetrics, Sestre milosrdnice University Hospital Center, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4885","published-online":{"date-parts":[[2023,12,15]]},"reference":[{"key":"key20231219080203_r1","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3390\/medicina55070384","article-title":"Key molecular events in cervical cancer development.","volume":"55","author":"Balasubramaniam","year":"2019","journal-title":"Medicina (Kaunas)"},{"key":"key20231219080203_r2","doi-asserted-by":"crossref","DOI":"10.6061\/clinics\/2018\/e548s","article-title":"Oxidative stress: Therapeutic approaches for cervical cancer treatment.","volume":"73","author":"Silva","year":"2018","journal-title":"Clinics (S\u00e3o Paulo)"},{"key":"key20231219080203_r3","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/S0009-9120(02)00376-4","article-title":"Circulating lipid peroxidation and antioxidant status in cervical cancer patients: a case-control study.","volume":"35","author":"Manju","year":"2002","journal-title":"Clin Biochem"},{"key":"key20231219080203_r4","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1097\/CEJ.0b013e328305a10b","article-title":"Ahmad Zailani Hatta, Dali AZHM, Ali SAM, Ngah WZW, Yusof YAM. 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