{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T00:25:06Z","timestamp":1718324706807},"reference-count":21,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2024,6,15]]},"abstract":"<jats:p>Graphical abstract\nHighlights\n\u2022 In vivo hemolysis due to C. perfringens infection occurred in an extremely short period\n\u2022 H index allowed monitoring severity of hemolysis and successful managing of samples\n\u2022 Laboratory procedure to investigate origin of hemolysis and determine C. perfringens as a cause of intravascular hemolysis is presented\nA patient presented with fever, severe pain and edematous tight due to hip trauma and was scheduled for urgent fasciotomy. Following physical examination, laboratory analyses were requested, and results revealed anemia and severe infection. As the patient\u2019s condition was serious, a new set of samples was sent to the laboratory four hours later. Following centrifugation, severely hemolyzed dark-colored serum and plasma samples were obtained and in vitro hemolysis was suspected. The collection of samples was repeated, but a new set of samples was also hemolyzed with a significant decrease in the hemoglobin value. At that point, in vivo hemolysis was suspected, and samples were processed according to standard laboratory procedures for hemolytic samples. Following confirmation of the gas gangrene diagnosis by clinicians, the cause of hemolysis was attributed to the cytotoxic activity of \u03b1-toxin produced by the anaerobic gram-positive bacterium Clostridium perfringens. An insight into the laboratory procedure that could help to narrow down the causes of hemolysis and single out C. perfringens as a cause of intravascular hemolysis was given.<\/jats:p>","DOI":"10.11613\/bm.2024.021001","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T09:17:14Z","timestamp":1713259034000},"page":"352-361","source":"Crossref","is-referenced-by-count":0,"title":["Hematological investigations in a case of intravascular hemolysis due to Clostridium perfringens infection"],"prefix":"10.11613","volume":"34","author":[{"given":"Ana","family":"Bronic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Pavi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anamarija","family":"Beljan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktorija","family":"Blagec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4885","published-online":{"date-parts":[[2024,6,15]]},"reference":[{"key":"key20240416051704_r1"},{"key":"key20240416051704_r2","doi-asserted-by":"crossref","first-page":"S93","DOI":"10.1086\/341928","article-title":"The role of clostridial toxins in the pathogenesis of gas gangrene.","volume":"35","author":"Stevens","year":"2002","journal-title":"Clin Infect Dis"},{"key":"key20240416051704_r3","doi-asserted-by":"crossref","first-page":"e295","DOI":"10.1515\/cclm-2020-0676","article-title":"Massive hemolysis due to Clostridium perfringens: a laboratory\u2019s perspective.","volume":"58","author":"Smit","year":"2020","journal-title":"Clin Chem Lab Med"},{"key":"key20240416051704_r4","first-page":"343","article-title":"Clostridium perfringens septicaemia with massive intravascular haemolysis: a case report and review of the literature.","volume":"68","author":"van Bunderen","year":"2010","journal-title":"Neth J Med"},{"key":"key20240416051704_r5","unstructured":"Sysmex. 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