{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:24:18Z","timestamp":1790270658634,"version":"4.1.0"},"reference-count":12,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:p>IntroductionAdrenocorticotropic hormone (ACTH) is a peptide secreted by pituitary gland that plays an important role in regulating cortisol secretion. Its determination is difficult because of instability in whole blood. Several factors that influence ACTH stability in blood before analysis have been identified: temperature, hemolysis, time to centrifugation and presence of protease inhibitors. Published results on ACTH whole blood stability seem contradictory.\nMaterials and methodsWe performed a stability study in 10 healthy volunteers. Three different conditions were tested: ethylenediaminetetraacetic acid (EDTA) at 4 \u00b0C, EDTA + aprotinin at 4 \u00b0C, EDTA + aprotinin at room temperature. Stability was evaluated for 8 hours. Adrenocorticotropic hormone measurements and hemolysis index were performed respectively on Cobas e602 and c701 (Roche Diagnostics, Mannheim, Germany). We compared percentage deviations with total change limit using a threshold of 7.5%.\nResultsWe showed that ACTH is stable 8 hours with EDTA at 4 \u00b0C, 4 hours with EDTA + aprotinin at 4 \u00b0C and 2 hours with EDTA + aprotinin at 22 \u00b0C.\nConclusionsAprotinin does not appear to give ACTH greater stability but can be used without exceeding 4 hours at 4 \u00b0C. Refrigerated pouch transport also seems to be more appropriate for ACTH in whole blood.<\/jats:p>","DOI":"10.11613\/bm.2024.030702","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T09:06:30Z","timestamp":1723626390000},"page":"451-456","source":"Crossref","is-referenced-by-count":3,"title":["Stability of adrenocorticotropic hormone in whole blood samples"],"prefix":"10.11613","volume":"34","author":[{"given":"Julie","family":"Fettig","sequence":"first","affiliation":[{"name":"General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u00efs","family":"Melin","sequence":"additional","affiliation":[{"name":"General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ry","family":"Brunel","sequence":"additional","affiliation":[{"name":"General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Gillibert","sequence":"additional","affiliation":[{"name":"Biostatistics Department, Rouen University Hospital, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Girot","sequence":"additional","affiliation":[{"name":"General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Fraissinet","sequence":"additional","affiliation":[{"name":"General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France; UNIROUEN, Normandie University, INSERM, U1239, NorDiC, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4885","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"key20240814050625_r1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1258\/000456303322326371","article-title":"Analytical and clinical aspects of adrenocorticotrophin determination.","volume":"40","author":"Talbot","year":"2003","journal-title":"Ann Clin Biochem"},{"key":"key20240814050625_r2","doi-asserted-by":"crossref","first-page":"3262","DOI":"10.1002\/pmic.200401245","article-title":"HUPO Plasma Proteome Project specimen collection and handling: towards the standardization of parameters for plasma proteome samples.","volume":"5","author":"Rai","year":"2005","journal-title":"Proteomics"},{"key":"key20240814050625_r3","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1080\/00365513.2016.1230887","article-title":"Whole blood samples for adrenocorticotrophic hormone measurement can be stored at room temperature for 4\u2005hours.","volume":"76","author":"Christensen","year":"2016","journal-title":"Scand J Clin Lab Invest"},{"key":"key20240814050625_r4","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1515\/cclm-2012-0431","article-title":"Adrenocorticotropic hormone stability in preanalytical phase depends on temperature and proteolytic enzyme inhibitor.","volume":"51","author":"Casati","year":"2013","journal-title":"Clin Chem Lab Med"},{"key":"key20240814050625_r5","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1515\/cclm-2020-0061","article-title":"The CRESS checklist for reporting stability studies: on behalf of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE).","volume":"59","author":"Cornes","year":"2020","journal-title":"Clin Chem Lab Med"},{"key":"key20240814050625_r6","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.clinbiochem.2012.01.012","article-title":"Stability study of 81 analytes in human whole blood, in serum and in plasma.","volume":"45","author":"Oddoze","year":"2012","journal-title":"Clin Biochem"},{"key":"key20240814050625_r7","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1038\/nrg1111","article-title":"Human and mouse proteases: a comparative genomic approach.","volume":"4","author":"Puente","year":"2003","journal-title":"Nat Rev Genet"},{"key":"key20240814050625_r8","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1093\/labmed\/lmw027","article-title":"The Effectiveness of Temperature Versus Aprotinin in Maintaining the Preanalytical Stability of Adrenocorticotrophin.","volume":"47","author":"Turkon","year":"2016","journal-title":"Lab Med"},{"key":"key20240814050625_r9","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1016\/j.clinbiochem.2010.09.020","article-title":"Stability of plasma adrenocorticotrophic hormone (ACTH): influence of hemolysis, rapid chilling, time, and the addition of a maleimide.","volume":"43","author":"Livesey","year":"2010","journal-title":"Clin Biochem"},{"key":"key20240814050625_r10","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.clinbiochem.2020.04.004","article-title":"Evaluation of plasma ACTH stability using the Roche Elecsys immunoassay.","volume":"81","author":"Nandakumar","year":"2020","journal-title":"Clin Biochem"},{"key":"key20240814050625_r11","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1177\/0004563216636898","article-title":"EDTA interference in electrochemiluminescence ACTH assay.","volume":"53","author":"Toprak","year":"2016","journal-title":"Ann Clin Biochem"},{"key":"key20240814050625_r12","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1515\/labmed-2023-0086","article-title":"Research on the stability changes in expert consensus of the ACTH detection preprocessing scheme.","volume":"48","author":"Dong","year":"2024","journal-title":"J Lab Med"}],"container-title":["Biochemia medica"],"original-title":[],"link":[{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/34\/3\/10.11613\/BM.2024.030702\/fullArticle","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T11:12:48Z","timestamp":1729595568000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/34\/3\/10.11613\/BM.2024.030702"}},"subtitle":["effects of storage conditions"],"short-title":[],"issued":{"date-parts":[[2024,10,15]]},"references-count":12,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,10,15]]},"published-print":{"date-parts":[[2024,10,15]]}},"URL":"https:\/\/doi.org\/10.11613\/bm.2024.030702","relation":{},"ISSN":["1846-7482","1330-0962"],"issn-type":[{"value":"1846-7482","type":"electronic"},{"value":"1330-0962","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10,15]]}}}