{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T02:23:55Z","timestamp":1785723835986,"version":"3.56.0"},"reference-count":9,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:p>IntroductionThyroid-stimulating hormone (TSH) is a glycoprotein secreted by the anterior pituitary gland and is regulated by negative feedback from the serum free thyroid hormones. In this study we aimed to quantitate the relative bias caused by calibration drifting as seen in our TSH Levey-Jennings quality control (QC) charts and assess the magnitude of bias on patients\u2019 samples.\nMaterials and methodsIn the period from October 2021 to August 2022 we looked at the QC results of ten 28-days\u2019 calibration time intervals and calculated relative bias compared to the mean. For each time interval the mean from three QC points before and after calibration was calculated. The average from 10 pre- and post-calibration means was calculated and the relative bias, pre- and post-calibration, was then calculated. We used 5 patient samples with low, normal and high TSH concentrations and calculated relative bias pre- and post-calibration. The allowed relative bias for TSH is \u00b1 6.7%.\nResultsAt both QC levels, with the respective means of 5.14 mIU\/L (coefficient of variation, CV% = 3.1%) and 27.80 mIU\/L (CV% = 3.2%) had their respective relative bias - 8.2% and - 7.9%. The patient samples with low (0.586 mIU\/L), normal (2.89 mIU\/L and 5.19 mIU\/L) and high (20.5 mIU\/L and 39.8 mIU\/L) TSH had - 4.1%, - 4.0%, - 3.5%, - 5.1% and - 4.1%, respectively.\nConclusionEven though the relative bias exceeded allowable criteria for the QC samples, this was not manifested on the patients\u2019 samples.<\/jats:p>","DOI":"10.11613\/bm.2024.030703","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T09:07:50Z","timestamp":1723626470000},"page":"457-461","source":"Crossref","is-referenced-by-count":1,"title":["The influence of calibration on bias in quality control and patient results for TSH on Vitros XT 7600 analyzer"],"prefix":"10.11613","volume":"34","author":[{"given":"Jill","family":"Boreyko","sequence":"first","affiliation":[{"name":"Hamilton Regional Laboratory Medicine Program (HRLMP), Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josko","family":"Ivica","sequence":"additional","affiliation":[{"name":"Hamilton Regional Laboratory Medicine Program (HRLMP), Hamilton, Ontario, Canada; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4885","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"key20240814050749_r1","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1210\/jcem-62-5-960","article-title":"Deficient nocturnal surge of thyrotropin in central hypothyroidism.","volume":"62","author":"Caron","year":"1986","journal-title":"J Clin Endocrinol Metab"},{"key":"key20240814050749_r2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.ecl.2017.04.002","article-title":"Biochemical Testing in Thyroid Disorders.","volume":"46","author":"Esfandiari","year":"2017","journal-title":"Endocrinol Metab Clin North Am"},{"key":"key20240814050749_r3","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1089\/thy.2012.0205","article-title":"Clinical practice guidelines for hypothyroidism in adults: cosponsored by the American Association of Clinical Endocrinologists and the American Thyroid Association.","volume":"22","author":"Garber","year":"2012","journal-title":"Thyroid"},{"key":"key20240814050749_r4","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1089\/thy.2007.0241","article-title":"Variation in thyroid function tests in patients with stable untreated subclinical hypothyroidism.","volume":"18","author":"Karmisholt","year":"2008","journal-title":"Thyroid"},{"key":"key20240814050749_r5","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1210\/jcem-70-2-453","article-title":"Applications of a new chemiluminometric thyrotropin assay to subnormal measurement.","volume":"20","author":"Spencer","year":"1990","journal-title":"J Clin Endocrinol Metab"},{"key":"key20240814050749_r6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3343\/alm.2019.39.1.3","article-title":"Laboratory Testing in Thyroid Conditions - Pitfalls and Clinical Utility.","volume":"39","author":"Soh","year":"2019","journal-title":"Ann Lab Med"},{"key":"key20240814050749_r7","first-page":"525","article-title":"Between and within calibration variation: implications for internal quality control rules.","volume":"55","author":"Limn","year":"2023","journal-title":"Pathology"},{"key":"key20240814050749_r8","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1053\/ajkd.2002.32765","article-title":"Calibration and random variation of the serum creatinine assay as critical elements of using equations to estimate glomerular filtration rate.","volume":"39","author":"Coresh","year":"2002","journal-title":"Am J Kidney Dis"},{"key":"key20240814050749_r9","unstructured":"Gallaher MP, Mobley LR, Klee GG, Schryver P. The Impact of Calibration Error in Medical Decision Making. Available from: https:\/\/www.nist.gov\/system\/files\/documents\/director\/planning\/report04-1.pdf. Accessed June 26th, 2024."}],"container-title":["Biochemia medica"],"original-title":[],"link":[{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/34\/3\/10.11613\/BM.2024.030703\/fullArticle","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T11:13:35Z","timestamp":1729595615000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biochemia-medica.com\/en\/journal\/34\/3\/10.11613\/BM.2024.030703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,15]]},"references-count":9,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,10,15]]},"published-print":{"date-parts":[[2024,10,15]]}},"URL":"https:\/\/doi.org\/10.11613\/bm.2024.030703","relation":{},"ISSN":["1846-7482","1330-0962"],"issn-type":[{"value":"1846-7482","type":"electronic"},{"value":"1330-0962","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10,15]]}}}