{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T15:47:01Z","timestamp":1777045621290,"version":"3.51.4"},"reference-count":13,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:p>This case report describes interference from heterophilic antibodies in D-dimer assay. The interference was suspected due to discrepancies between D-dimer concentrations in the original sample and diluted samples, as well as inconsistent clinical findings. The patient\u2019s medical history, laboratory results, and imaging studies were considered in the investigation. Heterophilic antibodies, likely developed during the SARS-CoV-2 infection, were identified as the probable cause of interference. The interference was confirmed through various methods, including dilution studies, blocking heterophilic antibodies, and comparing results with an alternative D-dimer method. This case highlights the importance of recognizing and addressing interference in D-dimer testing, emphasizing the need for collaboration between clinicians and laboratory specialists.<\/jats:p>","DOI":"10.11613\/bm.2024.031001","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T09:09:41Z","timestamp":1723626581000},"page":"509-515","source":"Crossref","is-referenced-by-count":1,"title":["D-dimer assay interference detected by the discrepancy in D-dimer concentrations at different dilutions"],"prefix":"10.11613","volume":"34","author":[{"given":"Vatroslav","family":"\u0160eri\u0107","sequence":"first","affiliation":[{"name":"Clinical Institute of Laboratory Diagnostics, University Hospital Centre Osijek, Osijek, Croatia; Department of Medicinal Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirna","family":"Kirin","sequence":"additional","affiliation":[{"name":"Siemens Healthineers, Siemens Healthcare d.o.o, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikica","family":"Buljanovi\u0107","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Laboratory, General Hospital Na\u0161ice, Na\u0161ice, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marija","family":"Mili\u0107","sequence":"additional","affiliation":[{"name":"Clinical Institute of Laboratory Diagnostics, University Hospital Centre Osijek, Osijek, Croatia; Department of Medicinal Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dejana","family":"Brki\u0107 Barbari\u0107","sequence":"additional","affiliation":[{"name":"Clinical Institute of Transfusion Medicine, University Hospital Centre Osijek, Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Luki\u0107","sequence":"additional","affiliation":[{"name":"Clinical Institute of Laboratory Diagnostics, University Hospital Centre Osijek, Osijek, Croatia;Department of Medicinal Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4885","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"key20240814050938_r1","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1002\/ajh.25482","article-title":"The D-dimer assay.","volume":"94","author":"Johnson","year":"2019","journal-title":"Am J Hematol"},{"key":"key20240814050938_r2","doi-asserted-by":"crossref","first-page":"50","DOI":"10.11613\/BM.2009.005","article-title":"Interferencije svojstvene kvantitativnim imunokemijskim metodama.","volume":"19","author":"Dodig","year":"2009","journal-title":"Biochem Med (Zagreb)"},{"key":"key20240814050938_r3","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.beem.2013.05.011","article-title":"Heterophilic antibody interference in immunometric assays.","volume":"27","author":"Bolstad","year":"2013","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"key":"key20240814050938_r4","first-page":"105","article-title":"Interferences in immunoassay.","volume":"25","author":"Tate","year":"2004","journal-title":"Clin Biochem Rev"},{"key":"key20240814050938_r5"},{"key":"key20240814050938_r6","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1515\/cclm-2021-1288","article-title":"Interferences in immunoassays: Review and practical algorithm.","volume":"60","author":"Wauthier","year":"2022","journal-title":"Clin Chem Lab Med"},{"key":"key20240814050938_r7","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1515\/tjb-2021-0262","article-title":"A case of falsely elevated D-dimer result.","volume":"47","author":"\u00c7evlik","year":"2022","journal-title":"Turkish J Biochem."},{"key":"key20240814050938_r8","doi-asserted-by":"crossref","first-page":"e13","DOI":"10.1111\/ijlh.13654","article-title":"Interference of D-dimer levels from heterophilic antibody in COVID-19: A serious concern in treatment and follow-up of patients.","volume":"44","author":"Ozbalci","year":"2022","journal-title":"Int J Lab Hematol"},{"key":"key20240814050938_r9","doi-asserted-by":"crossref","DOI":"10.1002\/jcla.22687","article-title":"What makes D-dimer assays suspicious\u2013heterophilic antibodies?","volume":"33","author":"Wu","year":"2019","journal-title":"J Clin Lab Anal"},{"key":"key20240814050938_r10","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1097\/MBC.0000000000000017","article-title":"Interference from heterophilic antibodies in D-dimer assessment. 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