{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T09:41:21Z","timestamp":1760694081423,"version":"build-2065373602"},"reference-count":36,"publisher":"Croatian Society for Medical Biochemistry and Laboratory Medicine","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. med. (Online)"],"published-print":{"date-parts":[[2025,10,15]]},"abstract":"<jats:p>Acute abdominal pain accounts for 7-10% of all emergency department visits. Appendicitis, being one of the leading causes of acute abdominal surgery, poses significant diagnostic challenges. Negative appendectomy rates can be as high as 40%, while complications occur in more than 90% if the diagnosis is missed during the initial examination. Therefore, more effective preoperative screening is required for patients with suspected appendicitis. Recent studies suggest that novel biomarkers, particularly leucine-rich alpha 2-glycoprotein and calprotectin, may improve the early and accurate diagnosis of acute appendicitis by demonstrating high specificity and sensitivity, respectively. Unlike C-reactive protein, the production of leucine-rich alpha 2-glycoprotein 1 and calprotectin takes place at the site of inflammation. As a result, their raised concentrations might be evident early in a disease, possibly before other common markers of inflammation start to rise. This literature review aims to assess the potential role of leucine-rich alpha 2-glycoprotein 1 and calprotectin as diagnostic biomarkers in patients with suspected acute appendicitis, acknowledging the need for additional data to fully assess their diagnostic accuracy.<\/jats:p>","DOI":"10.11613\/bm.2025.030504","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T08:22:07Z","timestamp":1760516527000},"page":"375-383","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic value of leucine-rich alpha-2-glycoprotein 1 and calprotectin in acute appendicitis"],"prefix":"10.11613","volume":"35","author":[{"given":"Lea","family":"Gvozdanovi\u0107","sequence":"first","affiliation":[{"name":"Emergency Department, General Hospital Na\u0161ice","place":["Na\u0161ice, Croatia"]},{"name":"Faculty of Medicine, Josip Juraj Strossmayer University of Osijek","place":["Osijek, Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u017deljka","family":"Dragila","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Josip Juraj Strossmayer University of Osijek","place":["Osijek, Croatia"]},{"name":"Emergency Department, University Hospital Center Osijek","place":["Osijek, Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luka","family":"Mar\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Emergency Department, General Hospital \u201cDr. Josip Ben\u010devi\u0107\"","place":["Slavonski Brod, Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Klapan","sequence":"additional","affiliation":[{"name":"Emergency Department, General Hospital Na\u0161ice","place":["Na\u0161ice, Croatia"]},{"name":"Faculty of Dental Medicine and Health Osijek, Josip Juraj Strossmayer University of Osijek","place":["Osijek, Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u017deljka","family":"Dujmi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia; Faculty of Dental Medicine and Health Osijek, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josip","family":"Samard\u017ei\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine and Health Osijek, Josip Juraj Strossmayer University of Osijek","place":["Osijek, Croatia"]},{"name":"Department of Abdominal Surgery, General Hospital \u201cDr. Josip Ben\u010devi\u0107\u201d","place":["Slavonski Brod, Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zrinka","family":"Mihaljevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek","place":["Osijek, Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vi\u0161nja","family":"Nesek-Adam","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine and Health Osijek, Josip Juraj Strossmayer University of Osijek","place":["Osijek, Croatia"]},{"name":"Department of Anesthesiology, Clinical Hospital \u201cSveti Duh\u201d","place":["Zagreb, Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4885","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"key20251015082201_r1","doi-asserted-by":"crossref","first-page":"362","DOI":"10.21037\/atm.2016.09.10","article-title":"Epidemiology and outcomes of acute abdominal pain in a large urban Emergency Department: retrospective analysis of 5,340 cases.","volume":"4","author":"Cervellin","year":"2016","journal-title":"Ann Transl Med"},{"key":"key20251015082201_r2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s13017-020-00306-3","article-title":"Diagnosis and treatment of acute appendicitis: 2020 update of the WSES Jerusalem guidelines.","volume":"15","author":"Di Saverio","year":"2020","journal-title":"World J Emerg Surg"},{"key":"key20251015082201_r3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/bjs.11440","article-title":"RIFT Study Group on behalf of the West Midlands Research Collaborative. 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