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The function of ChP vessels has been implicated in waste clearance efficiency during aging and neurodegenerative diseases. At present, postmortem studies are the main method to assess choroid plexus vascular integrity, with a few tools to measure ChP function in living humans. Here, we proposed a non-invasive MRI approach to assess ChP vascular elasticity based on the detection of MRI signal changes in response to vasoactive challenges. The mechanism of the signal is hypothesized to be due to reciprocal blood and stroma volume alterations during vessel expansion. We demonstrated that ChP vascular elasticity can be evaluated with BOLD MRI using a hypercapnia challenge of CO2 inhalation. This effect is specifically located in the brain ventricles where ChP is abundant. We revealed the ability of the technique in detecting age-related reduction in ChP vascular elasticity. We further showed that this effect can be assessed with gas-free methods, including intermittent breath modulation and resting-state BOLD fMRI. We characterized the image contrast requirement under which this effect can be detected. This technique may provide a clinically feasible tool for assessing ChP vascular function in health and disease.<\/jats:p>","DOI":"10.1162\/imag.a.1216","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T19:42:52Z","timestamp":1774986172000},"update-policy":"https:\/\/doi.org\/10.1162\/mitpressjournals.corrections.policy","source":"Crossref","is-referenced-by-count":0,"title":["Non-invasive MRI of choroid plexus vascular function"],"prefix":"10.1162","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0830-5960","authenticated-orcid":false,"given":"Peiying","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Diagnostic Radiology & Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori","family":"Donaldson","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology & Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beini","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology & Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gagan S.","family":"Wig","sequence":"additional","affiliation":[{"name":"Center for Vital Longevity & Department of Psychology, The University of Texas at Dallas, Dallas, TX, United States"},{"name":"Department of Psychiatry, The University of Texas Southwestern Medical Center, Dallas, TX, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanzhang","family":"Lu","sequence":"additional","affiliation":[{"name":"The Russell H. Morgan Department of Radiology & Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"281","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"issue":"5","key":"2026041715565861800_IMAG.a.1216-b1","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1177\/1971400917714280","article-title":"Diffusion-weighted magnetic resonance imaging in the assessment of choroid plexus aging","volume":"30","author":"Alicioglu","year":"2017","journal-title":"Neuroradiol J"},{"key":"2026041715565861800_IMAG.a.1216-b2","doi-asserted-by":"crossref","first-page":"734992","DOI":"10.3389\/fnagi.2021.734992","article-title":"Characterization of age-related differences in the human choroid plexus volume, microstructural integrity, and blood perfusion using multiparameter magnetic resonance imaging","volume":"13","author":"Alisch","year":"2021","journal-title":"Front Aging 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