{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:12:50Z","timestamp":1782835970191,"version":"3.54.5"},"reference-count":66,"publisher":"Walter de Gruyter GmbH","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Benef. Microbes"],"published-print":{"date-parts":[[2023,12,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:italic>Rhodobacter<\/jats:italic> species are promising beneficial microbes that can improve growth performance, immunity and antioxidant capability in aquatic crustaceans. Yet the safety of <jats:italic>Rhodobacter azotoformans<\/jats:italic> for potential application in Chinese mitten crab <jats:italic>Eriocheir sinensis<\/jats:italic> is still unclear. In the present study, <jats:italic>R. azotoformans<\/jats:italic> SY5, a potential probiotic additive that can significantly improve the growth performance, immunity, antioxidant capability, and disease resistance in <jats:italic>E. sinensis<\/jats:italic>, was evaluated for safety through whole genome sequencing, antibiotic resistance, toxic metabolites, virulence, and crab tolerance assays. The results indicated that <jats:italic>R. azotoformans<\/jats:italic> SY5 only harboured the acyl carrier protein-encoding gene (<jats:italic>acpP<\/jats:italic>) that was universal in probiotic bacteria with the function of bacterial fatty acid biosynthesis, exhibited high susceptibility to aminoglycosides, penicillins, polymyxins, polyphosphates, quinolones, and tetracyclines antimicrobials, and possessed inability to produce hemolysin, hydrogen sulphide, nitrite, ammonia, and phenylpyruvate. In addition, <jats:italic>R. azotoformans<\/jats:italic> SY5 showed no pathogenicity for <jats:italic>E. sinensis<\/jats:italic> with the seven-day acute intraperitoneal LD<jats:sub>50<\/jats:sub> value of above 6.0\u00a0\u00d7 10<jats:sup>9<\/jats:sup> cfu\/ml and 30-day chronic oral LD<jats:sub>50<\/jats:sub> of above 6.0\u00a0\u00d7 10<jats:sup>9<\/jats:sup> cfu\/g diet. To our knowledge, this is the first report on the safety of <jats:italic>R. azotoformans<\/jats:italic> for potential application in Chinese mitten crabs.<\/jats:p>","DOI":"10.1163\/18762891-20230086","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T09:02:58Z","timestamp":1702544578000},"page":"641-651","source":"Crossref","is-referenced-by-count":4,"title":["Safety assessment of Rhodobacter azotoformans SY5 for potential application in Chinese mitten crab Eriocheir sinensis"],"prefix":"10.1163","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8386-5138","authenticated-orcid":true,"given":"H.","family":"Cao","sequence":"first","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China P.R."},{"name":"Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs of China, Shanghai 201306, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6954-0636","authenticated-orcid":true,"given":"S.","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China P.R."},{"name":"Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs of China, Shanghai 201306, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7992-115X","authenticated-orcid":true,"given":"X.","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China P.R."},{"name":"Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs of China, Shanghai 201306, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2741-4521","authenticated-orcid":true,"given":"L.","family":"Xu","sequence":"additional","affiliation":[{"name":"Marine Science Research Institute of Shandong Province (National Oceanographic Center), Qingdao 266104, China P.R."},{"name":"Shandong Key Laboratory of Disease Control in Mariculture, Qingdao 266104, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7464-2198","authenticated-orcid":true,"given":"J.","family":"Diao","sequence":"additional","affiliation":[{"name":"Marine Science Research Institute of Shandong Province (National Oceanographic Center), Qingdao 266104, China P.R."},{"name":"Shandong Key Laboratory of Disease Control in Mariculture, Qingdao 266104, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3210-0987","authenticated-orcid":true,"given":"Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Marine Science Research Institute of Shandong Province (National Oceanographic Center), Qingdao 266104, China P.R."},{"name":"Shandong Key Laboratory of Disease Control in Mariculture, Qingdao 266104, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6444-6533","authenticated-orcid":true,"given":"C.","family":"Gai","sequence":"additional","affiliation":[{"name":"Marine Science Research Institute of Shandong Province (National Oceanographic Center), Qingdao 266104, China P.R."},{"name":"Shandong Key Laboratory of Disease Control in Mariculture, Qingdao 266104, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7690-4734","authenticated-orcid":true,"given":"H.","family":"Ye","sequence":"additional","affiliation":[{"name":"Marine Science Research Institute of Shandong Province (National Oceanographic Center), Qingdao 266104, China P.R."},{"name":"Shandong Key Laboratory of Disease Control in Mariculture, Qingdao 266104, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"R000001","series-title":"Veterinary World","first-page":"34","article-title":"Incidence and virulence characteristics of Aeromonas spp. in fish","volume":"10","author":"Abd-El-Malek, A.M.","unstructured":"Abd-El-Malek, A.M., 2017. 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