{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:12:11Z","timestamp":1790831531329,"version":"4.1.0"},"reference-count":62,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T00:00:00Z","timestamp":1715644800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"crossref","award":["899546"],"award-info":[{"award-number":["899546"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Minist\u00e8re de l'Agriculture, de l'Agroalimentaire et de la For\u00eat","award":["PRIM"],"award-info":[{"award-number":["PRIM"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Benef. Microbes"],"published-print":{"date-parts":[[2024,5,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p>Bovine mastitis (BM) is a major disease in dairy industry. The current approaches\u00a0\u2013 mainly antibiotic treatments\u00a0\u2013 are not entirely effective and may contribute to antimicrobial resistance dissemination, rising the need for alternative treatment. The present study aims to evaluate the impact of post-milking application of <jats:italic>Lacticaseibacillus paracasei<\/jats:italic> CIRM BIA 1542 (Lp1542) on the teat skin (TS) of 20 Holstein cows in mid lactation, in order to reinforce the barrier effect of the microbiota naturally present on the teat. Treatment (Lp1542, iodine or no treatment) was applied post-milking twice a day on the 4 teats of healthy animals for 15 days. Blood and milk samples, and TS swabs were collected at day (D)1, D8, D15 and D26 before morning milking and at D15 before evening milking (D15E) to evaluate Lp1542 impact at the microbial, immune and physiological levels. Lp1542 treatment resulted in a higher lactic acid bacteria and total microbial populations on TS and in foremilk (FM) at D15(E) compared with iodine treatment. Metabarcoding analysis revealed changes in the composition of TS and FM microbiota, beyond a higher <jats:italic>Lacticaseibacillus<\/jats:italic> abundance. This included a higher abundance of <jats:italic>Actinobacteriota<\/jats:italic>, including <jats:italic>Bifidobacterium<\/jats:italic>, and a lower abundance of <jats:italic>Pseudomonadota<\/jats:italic> on TS of Lp1542 compared with iodine-treated quarters. In addition, Lp1542 treatment did not trigger any major inflammatory response in the mammary gland, except interleukin 8 production and expression which tended to be slightly higher in Lp1542-treated cows compared with the others. Finally, Lp1542 treatment had no impact on the mammary epithelium functionality (milk yield and composition) and integrity (epithelial cell exfoliation into milk and milk Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup> ratio). Altogether, these results indicate that a topical treatment with Lp1542 is safe with regard to mammary gland physiology and immune system, while impacting its microbiota, inviting us to further explore its effectiveness for mastitis prevention.<\/jats:p>","DOI":"10.1163\/18762891-bja00014","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T11:59:58Z","timestamp":1715687998000},"page":"275-291","source":"Crossref","is-referenced-by-count":4,"title":["Post-milking application of a Lacticaseibacillus paracasei strain impacts bovine teat microbiota while preserving the mammary gland physiology and immunity"],"prefix":"10.1163","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5073-1141","authenticated-orcid":true,"given":"C.","family":"Goetz","sequence":"first","affiliation":[{"name":"INRAE, L\u2019Institut Agro Rennes-Angers, UMR 1253 STLO, Rennes Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Rault","sequence":"additional","affiliation":[{"name":"INRAE, L\u2019Institut Agro Rennes-Angers, UMR 1253 STLO, Rennes Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Cuffel","sequence":"additional","affiliation":[{"name":"INRAE, L\u2019Institut Agro Rennes-Angers, UMR 1253 STLO, Rennes Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Poton","sequence":"additional","affiliation":[{"name":"INRAE, L\u2019Institut Agro Rennes-Angers, UMR 1348 PEGASE, 35590 Saint-Gilles, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9680-7276","authenticated-orcid":true,"given":"L.","family":"Finot","sequence":"additional","affiliation":[{"name":"INRAE, L\u2019Institut Agro Rennes-Angers, UMR 1348 PEGASE, 35590 Saint-Gilles, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Boullet","sequence":"additional","affiliation":[{"name":"INRAE, L\u2019Institut Agro Rennes-Angers, UMR 1348 PEGASE, 35590 Saint-Gilles, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0820-0966","authenticated-orcid":true,"given":"M.","family":"Boutinaud","sequence":"additional","affiliation":[{"name":"INRAE, L\u2019Institut Agro Rennes-Angers, UMR 1348 PEGASE, 35590 Saint-Gilles, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6836-2855","authenticated-orcid":true,"given":"S.","family":"Even","sequence":"additional","affiliation":[{"name":"INRAE, L\u2019Institut Agro Rennes-Angers, UMR 1253 STLO, Rennes Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"R000001","series-title":"International Journal of Molecular Sciences","article-title":"Bifidobacterium bifidum enhances the intestinal epithelial tight junction barrier and protects against intestinal inflammation by targeting the Toll-like receptor-2 pathway in an NF-\u03baB-independent manner","volume":"22","author":"Al-Sadi, R.","unstructured":"Al-Sadi, R., Dharmaprakash, V., Nighot, P., Guo, S., Nighot, M., Do, T. and Ma, T.Y., 2021. 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Mastitis control in dairy herds: An illustrated and practical guide. Farming Press Books, Ipswich, UK."},{"key":"R000011","series-title":"Journal of Food Protection","first-page":"1658","article-title":"Iodine concentration in milk sampled from Canadian farms","volume":"73","author":"Borucki Castro, S.I.","unstructured":"Borucki Castro, S.I., Berthiaume, R., Laffey, P., Fouquet, A., Beraldin, F., Robichaud, A. and Lacasse, P., 2010. Iodine concentration in milk sampled from Canadian farms. Journal of Food Protection 73: 1658-1663. https:\/\/doi.org\/10.4315\/0362-028x-73.9.1658"},{"key":"R000012","series-title":"Journal of Dairy Science","first-page":"213","article-title":"Effects of iodine intake and teat-dipping practices on milk iodine concentrations in dairy cows","volume":"95","author":"Borucki Castro, S.I.","unstructured":"Borucki Castro, S.I., Berthiaume, R., Robichaud, A. and Lacasse, P., 2012. 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