{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:04:02Z","timestamp":1789790642762,"version":"4.0.1"},"reference-count":21,"publisher":"Walter de Gruyter GmbH","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Benef. Microbes"],"published-print":{"date-parts":[[2026,9,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Aging is associated with metabolic decline, brown adipose tissue (BAT) dysfunction, and remodelling of the gut microbiota.\n                    <jats:italic>Akkermansia muciniphila<\/jats:italic>\n                    (AKK) has been implicated in metabolic regulation; however, its relationship with BAT aging remains unclear. This study aims to investigate whether age-associated AKK depletion is linked to BAT aging and whether AKK supplementation attenuates aging phenotypes in BAT. Male mice aged 7 weeks, 7 months, and 11 months were analysed to characterise age-related changes in gut microbiota and BAT. 16S rRNA sequencing was performed to assess microbial composition. BAT morphology, thermogenic gene expression, and senescence markers were evaluated by histology, immunofluorescence, western blotting, and quantitative PCR. In a separate experiment, 11-month-old mice received live AKK supplementation for 30 days. Analysis of covariance was conducted to adjust for body weight where appropriate. Aging was accompanied by reduced microbial diversity and progressive depletion of AKK. BAT from older mice exhibited increased expression of senescence-associated genes, enlarged lipid droplets, and reduced thermogenic markers. AKK abundance was negatively correlated with BAT senescence markers. Supplementation with live AKK in middle-aged mice was associated with reduced Cdkn1a expression and increased thermogenic gene expression in BAT. These associations remained significant after adjustment for body weight. Aging in mice is accompanied by gut microbiota remodelling characterised by AKK decline and concurrent BAT aging phenotypes. AKK supplementation is associated with partial attenuation of BAT aging markers. These findings support a link between age-related microbial shifts and adipose tissue aging.\n                  <\/jats:p>","DOI":"10.1163\/18762891-bja00135","type":"journal-article","created":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:20:33Z","timestamp":1789788033000},"page":"1-14","source":"Crossref","is-referenced-by-count":0,"title":["Age-associated Akkermansia muciniphila decline links to brown adipose tissue aging: AKK supplementation improves BAT metabolic function"],"prefix":"10.1163","author":[{"given":"S.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Geriatrics, Nanjing Drum Tower Hospital, Drum Tower Clinical College, https:\/\/dx.doi.org\/12461Nanjing Medical University, Nanjing, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Nanjing Drum Tower Hospital, Drum Tower Clinical College, https:\/\/dx.doi.org\/12461Nanjing Medical University, Nanjing, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Nanjing Drum Tower Hospital, Drum Tower Clinical College, Nanjing University of Chinese Medicine, Nanjing, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Long","sequence":"additional","affiliation":[{"name":"School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Cui","sequence":"additional","affiliation":[{"name":"Nanjing Maternity and Child Health Care Hospital, Women\u2019s Hospital of Nanjing Medical University, Nanjing, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Nanjing Drum Tower Hospital, Nanjing, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Zhong","sequence":"additional","affiliation":[{"name":"Nanjing Maternity and Child Health Care Hospital, Women\u2019s Hospital of Nanjing Medical University, Nanjing, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Nanjing Drum Tower Hospital, Drum Tower Clinical College, https:\/\/dx.doi.org\/12461Nanjing Medical University, Nanjing, China P.R."},{"name":"Department of Chemistry, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, China P.R."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"R000001","series-title":"Nature Reviews Gastroenterology and Hepatology","first-page":"625","article-title":"Akkermansia muciniphila: paradigm for next-generation beneficial microorganisms","volume":"19","author":"Cani, P.D.","unstructured":"Cani, P.D., Depommier, C., Derrien, M., Everard, A., Druart, C., Belzer, C., Knauf, C., Burcelin, R., Langin, D., Seeley, R.J., Dhahbi, J.M., Francois, M., Bruneau, A., Charriere, S., Marques, T.M., Bellido, C., Neyrinck, A.M. and Delzenne, N.M., 2022. 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Physiological Reviews 84: 277-359. https:\/\/doi.org\/10.1152\/physrev.00015.2003"},{"key":"R000003","series-title":"Nature Medicine","first-page":"1096","article-title":"Supplementation with Akkermansia muciniphila in overweight and obese human volunteers: a proof-of-concept exploratory study","volume":"25","author":"Depommier, C.","unstructured":"Depommier, C., Everard, A., Druart, C., Plovier, H., Maiter, D., Baele, G., Neyrinck, A.M., Lambert, T., Bouche, C., Francois, M., Clement, K., Burcelin, R. and Cani, P.D., 2019. Supplementation with Akkermansia muciniphila in overweight and obese human volunteers: a proof-of-concept exploratory study. Nature Medicine 25: 1096-1103. https:\/\/doi.org\/10.1038\/s41591-019-0495-2"},{"key":"R000004","series-title":"International Journal of Systematic and Evolutionary Microbiology","first-page":"1469","article-title":"Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium","volume":"54","author":"Derrien, M.","unstructured":"Derrien, M., Vaughan, E.E., Plugge, C.M. and de Vos, W.M., 2004. Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium. International Journal of Systematic and Evolutionary Microbiology 54: 1469-1476. https:\/\/doi.org\/10.1099\/ijs.0.02873-0"},{"key":"R000005","series-title":"Proceedings of the National Academy of Sciences of the USA","first-page":"9066","article-title":"Crosstalk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity","volume":"110","author":"Everard, A.","unstructured":"Everard, A., Belzer, C., Geurts, L., Ouwerkerk, J.P., Druart, C., Bindels, L.B., Guiot, Y., Derrien, M., Muccioli, G.G., Delzenne, N.M., de Vos, W.M. and Cani, P.D., 2013. Crosstalk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity. Proceedings of the National Academy of Sciences of the USA 110: 9066-9071. https:\/\/doi.org\/10.1073\/pnas.1219451110"},{"key":"R000006","series-title":"Nature Reviews Endocrinology","first-page":"576","article-title":"Inflammaging: a new immune-metabolic viewpoint for age-related diseases","volume":"14","author":"Franceschi, C.","unstructured":"Franceschi, C., Garagnani, P., Parini, P., Giuliani, C., Santoro, A., Bonafe\u0300, M., Paolisso, G., Melis, T., Caiafa, P., Savarese, G., Sepe, F., Salvioli, S., Giovagnetti, A., Monti, D., Giunta, S., Olivieri, F., Candore, G., Caruso, C., Lio, D. and Passarino, G., 2018. Inflammaging: a new immune-metabolic viewpoint for age-related diseases. Nature Reviews Endocrinology 14: 576-590. https:\/\/doi.org\/10.1038\/s41574-018-0059-4"},{"key":"R000007","series-title":"Aging","first-page":"6375","article-title":"Oral administration of Akkermansia muciniphila elevates systemic antiaging and anticancer metabolites","volume":"13","author":"Grajeda-Iglesias, C.","unstructured":"Grajeda-Iglesias, C., Durand, S., Daille\u0300re, R., Michaud, M., Roberti, M., Routy, J., Be\u0301langer, M., Martin, J., Daignault, A., Labonte\u0301, F., Gagnon, J.F., Chabaud, M., Lebel, M., Verreault, D., St-Pierre, J., Marette, A., Bouchard, L. and Villani, A.C., 2021. Oral administration of Akkermansia muciniphila elevates systemic antiaging and anticancer metabolites. Aging 13: 6375-6405. https:\/\/doi.org\/10.18632\/aging.202739"},{"key":"R000008","series-title":"Nutrients","article-title":"Pasteurized Akkermansia muciniphila HB05 (HB05P) improves muscle strength and function: a 12-week, randomized, double-blind, placebo-controlled clinical trial","volume":"16","author":"Kang, C.H.","unstructured":"Kang, C.H., Jung, E.S., Jung, S.J., Park, S.Y., Lee, J.H., Kim, J.H., Lee, S.H., Lee, S.Y., Park, Y.K., Park, J.H., Kim, D.H., Kim, Y.J., Kim, J.S., Lee, J.Y., Kim, Y.H., Kim, J.H., Lee, J.W., Kim, H.J., Park, K.S. and Lee, S.K., 2024. Pasteurized Akkermansia muciniphila HB05 (HB05P) improves muscle strength and function: a 12-week, randomized, double-blind, placebo-controlled clinical trial. Nutrients 16: 4037. https:\/\/doi.org\/10.3390\/nu16234037"},{"key":"R000009","series-title":"International Journal of Molecular Sciences","article-title":"Comparative transcriptome profiling of young and old brown adipose tissue thermogenesis","volume":"22","author":"Kim, Y.","unstructured":"Kim, Y., Kang, B.E., Ryu, D., Lee, S., Park, J., Kim, H.J., Kim, K.T. and Lee, M.S., 2021. Comparative transcriptome profiling of young and old brown adipose tissue thermogenesis. International Journal of Molecular Sciences 22: 13143. https:\/\/doi.org\/10.3390\/ijms222313143"},{"key":"R000010","series-title":"Trends in Endocrinology and Metabolism","first-page":"107","article-title":"New developments in adipogenesis","volume":"20","author":"Lefterova, M.I.","unstructured":"Lefterova, M.I. and Lazar, M.A., 2009. New developments in adipogenesis. Trends in Endocrinology and Metabolism 20: 107-114. https:\/\/doi.org\/10.1016\/j.tem.2008.11.005"},{"key":"R000011","series-title":"Cell","first-page":"33","article-title":"Hallmarks of health","volume":"184","author":"Lo\u0301pez-Ot\u0131\u0301n, C.","unstructured":"Lo\u0301pez-Ot\u0131\u0301n, C. and Kroemer, G., 2021. Hallmarks of health. Cell 184: 33-63. https:\/\/doi.org\/10.1016\/j.cell.2020.11.034"},{"key":"R000012","series-title":"Cell","first-page":"1194","article-title":"The hallmarks of aging","volume":"153","author":"Lo\u0301pez-Ot\u0131\u0301n, C.","unstructured":"Lo\u0301pez-Ot\u0131\u0301n, C., Blasco, M.A., Partridge, L., Serrano, M. and Kroemer, G., 2013. The hallmarks of aging. Cell 153: 1194-1217. https:\/\/doi.org\/10.1016\/j.cell.2013.05.039"},{"key":"R000013","series-title":"Frontiers in Microbiology","article-title":"Whole-genome sequencing and genomic analysis of four Akkermansia strains newly isolated from human feces","volume":"15","author":"Lu, W.","unstructured":"Lu, W., Zha, B., Lyu, J., Liu, X., Zhang, Y., Chen, Y., Wang, J., Li, M., Zhao, L. and Zhang, H., 2024. Whole-genome sequencing and genomic analysis of four Akkermansia strains newly isolated from human feces. 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Scientific Reports 11: 19928. https:\/\/doi.org\/10.1038\/s41598-021-99362-1"},{"key":"R000015","series-title":"Nature Medicine","first-page":"107","article-title":"A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice","volume":"23","author":"Plovier, H.","unstructured":"Plovier, H., Everard, A., Druart, C., Depommier, C., Van Hul, M., Vieira-Silva, S., Falony, G., Raes, J., Maiter, D., Delzenne, N.M., de Vos, W.M. and Cani, P.D., 2017. A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice. Nature Medicine 23: 107-113. https:\/\/doi.org\/10.1038\/nm.4236"},{"key":"R000016","series-title":"Biochemical Pharmacology","article-title":"White adipose tissue dysfunction in obesity and aging","volume":"192","author":"Reyes-Farias, M.","unstructured":"Reyes-Farias, M., Fos-Domenech, J., Serra, D. and Palou, A., 2021. White adipose tissue dysfunction in obesity and aging. Biochemical Pharmacology 192: 114723. https:\/\/doi.org\/10.1016\/j.bcp.2021.114723"},{"key":"R000017","series-title":"Nature Reviews Endocrinology","first-page":"507","article-title":"The endocrine function of adipose tissues in health and cardiometabolic disease","volume":"15","author":"Scheja, L.","unstructured":"Scheja, L. and Heeren, J., 2019. The endocrine function of adipose tissues in health and cardiometabolic disease. Nature Reviews Endocrinology 15: 507-524. https:\/\/doi.org\/10.1038\/s41574-019-0230-6"},{"key":"R000018","series-title":"Journal of Clinical Investigation","first-page":"966","article-title":"Cellular senescence and the senescent secretory phenotype: therapeutic opportunities","volume":"123","author":"Tchkonia, T.","unstructured":"Tchkonia, T., Zhu, Y., van Deursen, J., Campisi, J. and Kirkland, J.L., 2013. Cellular senescence and the senescent secretory phenotype: therapeutic opportunities. Journal of Clinical Investigation 123: 966-972. https:\/\/doi.org\/10.1172\/JCI64098"},{"key":"R000019","series-title":"Pharmacological Research","article-title":"RNA sequencing reveals perivascular adipose tissue plasticity in response to angiotensin II","volume":"178","author":"Wang, Z.","unstructured":"Wang, Z., Lu, H., Garcia-Barrio, M., Villarreal-Davila, A., Sanchez-Lozada, L.G., Johnson, R.J. and Roncal-Jimenez, C., 2022. RNA sequencing reveals perivascular adipose tissue plasticity in response to angiotensin II. Pharmacological Research 178: 106183. https:\/\/doi.org\/10.1016\/j.phrs.2022.106183"},{"key":"R000020","series-title":"Nature Microbiology","first-page":"563","article-title":"Akkermansia muciniphila secretes a glucagon-like peptide-1-inducing protein that improves glucose homeostasis and ameliorates metabolic disease in mice","volume":"6","author":"Yoon, H.S.","unstructured":"Yoon, H.S., Cho, C.H., Yun, M.S., Lee, S.Y., Kim, B.S., Kim, D., Kim, H.J., Kim, H., Kim, S.Y., Lee, H.J., Park, J.H., Lee, J.S., Lee, D.K., Kim, J.K., Kim, J.H. and Bae, J.S., 2021. Akkermansia muciniphila secretes a glucagon-like peptide-1-inducing protein that improves glucose homeostasis and ameliorates metabolic disease in mice. Nature Microbiology 6: 563-573. https:\/\/doi.org\/10.1038\/s41564-021-00880-5"},{"key":"R000021","series-title":"ACS Nano","first-page":"26807","article-title":"Gastrointestinal self-adaptive and nutrient self-sufficient Akkermansia-gelatin porous microgels for synergistic therapy of ulcerative colitis","volume":"18","author":"Zhang, Y.","unstructured":"Zhang, Y., Wang, Y., Zhang, X., Liu, J., Zhang, Y., Li, X., Wang, C., Zhang, L., Chen, X. and Zhang, Y., 2024. Gastrointestinal self-adaptive and nutrient self-sufficient Akkermansia-gelatin porous microgels for synergistic therapy of ulcerative colitis. ACS Nano 18: 26807-26827. https:\/\/doi.org\/10.1021\/acsnano.4c07658"}],"container-title":["Beneficial Microbes"],"original-title":[],"link":[{"URL":"https:\/\/brill.com\/view\/journals\/bm\/aop\/article-10.1163-18762891-bja00135\/article-10.1163-18762891-bja00135.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/aop\/article-10.1163-18762891-bja00135\/article-10.1163-18762891-bja00135.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:22:34Z","timestamp":1789788154000},"score":1,"resource":{"primary":{"URL":"https:\/\/brill.com\/view\/journals\/bm\/aop\/article-10.1163-18762891-bja00135\/article-10.1163-18762891-bja00135.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,17]]},"references-count":21,"URL":"https:\/\/doi.org\/10.1163\/18762891-bja00135","relation":{},"ISSN":["1876-2891"],"issn-type":[{"value":"1876-2891","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,17]]}}}