{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T03:24:58Z","timestamp":1761362698524,"version":"build-2065373602"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Amphib Reptilia"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>\n                    <jats:italic>Pyxis arachnoides<\/jats:italic>\n                    is a Critically Endangered Malagasy tortoise for which little data about its natural history and genetic variation are available. This lack of knowledge complicates undertaking conservation measures considering\n                    <jats:italic>Pyxis<\/jats:italic>\n                    genetic diversity. Here, we describe six new microsatellites for\n                    <jats:italic>Pyxis arachnoides<\/jats:italic>\n                    from wild specimens and several localities, and the cross amplification of two\n                    <jats:italic>Astrochelys radiata<\/jats:italic>\n                    microsatellites. Of these microsatellites, two are monomorphic among the three distinct subspecies and a closely related species, while the remaining six are polymorphic. Microsatellite data are needed for this species to study the inter- and intra-subspecies genetic diversity, especially for the highly endangered subspecies\n                    <jats:italic>P. a. brygooi<\/jats:italic>\n                    , which currently occurs only in remote localities. The availability of polymorphic microsatellites will allow identifying the population of origin of individuals and will aid in justifying their management as separate evolutionary lineages for conservation purposes. The rationale is to preserve both evolutionary processes and adaptive potential.\n                  <\/jats:p>","DOI":"10.1163\/15685381-00002866","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T21:29:09Z","timestamp":1359581349000},"page":"125-128","source":"Crossref","is-referenced-by-count":3,"title":["Isolation and characterization of six polymorphic microsatellite loci for the Malagasy spider tortoise, Pyxis arachnoides and cross-amplification in Pyxis planicauda"],"prefix":"10.1163","volume":"34","author":[{"given":"Pablo","family":"Orozco-terWengel","sequence":"first","affiliation":[{"name":"1School of Biosciences, Cardiff University, Biomedical Science Building, Museum Avenue, Cardiff CF10 3AX, Wales, UK"}]},{"given":"Ylenia","family":"Chiari","sequence":"additional","affiliation":[{"name":"2CIBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"}]},{"given":"David\u00a0R.","family":"Vieites","sequence":"additional","affiliation":[{"name":"2CIBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"},{"name":"3Museo Nacional de Ciencias Naturales, Consejo Superior de Investigaciones Cient\u00edficas (CSIC), c\/Jos\u00e9 Guti\u00e9rrez Abascal 2, 28006 Madrid, Spain"}]},{"given":"Miguel","family":"Pedrono","sequence":"additional","affiliation":[{"name":"4CIRAD, UPR AGIRs, Antananarivo 101, Madagascar and CIRAD, UPR AGIRs, 34398 Montpellier, France"}]},{"given":"Edward","family":"Louis\u00a0Jr.","sequence":"additional","affiliation":[{"name":"5Omaha\u2019s Henry Doorly Zoo and Aquarium\u2019s Center for Conservation and Research, Omaha, Nebraska, USA"}]}],"member":"374","container-title":["Amphibia-Reptilia"],"original-title":[],"link":[{"URL":"https:\/\/brill.com\/view\/journals\/amre\/34\/1\/article-p125_14.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/amre\/34\/1\/article-p125_14.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/amre\/34\/1\/article-p125_14.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T03:09:58Z","timestamp":1761361798000},"score":1,"resource":{"primary":{"URL":"https:\/\/brill.com\/view\/journals\/amre\/34\/1\/article-p125_14.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1163\/15685381-00002866","relation":{},"ISSN":["0173-5373","1568-5381"],"issn-type":[{"type":"print","value":"0173-5373"},{"type":"electronic","value":"1568-5381"}],"subject":[],"published":{"date-parts":[[2013]]}}}