{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T10:01:24Z","timestamp":1761732084587,"version":"build-2065373602"},"reference-count":24,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Amphib Reptilia"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>\n                    Patterns of biodiversity and evolutionary processes controlling them are still poorly studied in desert biomes. Fine-scale markers could help answer some of the pressing research questions for desert biomes and Sahara in particular. Such markers are available for some large mammals and crocodiles, but not for small vertebrates. Here we present a battery of microsatellite loci developed for\n                    <jats:italic>Agama boulengeri<\/jats:italic>\n                    , a promising model to study evolutionary and demographic processes in the Sahara-Sahel. Loci were selected by sequencing enriched DNA libraries with 454 pyrosequencing. A\u00a0total of 23 polymorphic loci were successfully amplified in four multiplex reactions. Cross-amplification of the microsatellite loci in\n                    <jats:italic>A.\u00a0agama<\/jats:italic>\n                    and\n                    <jats:italic>A.\u00a0boueti<\/jats:italic>\n                    was partially successful. These markers are a promising tool for assessing genetic diversity, gene-flow dynamics and demographic patterns in this group. Given the genus\n                    <jats:italic>Agama<\/jats:italic>\n                    is distributed throughout Africa, results presented here might also facilitate studies in other regions.\n                  <\/jats:p>","DOI":"10.1163\/15685381-00003048","type":"journal-article","created":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T14:15:12Z","timestamp":1461680112000},"page":"246-252","source":"Crossref","is-referenced-by-count":0,"title":["Development of 23 microsatellite loci for Boulenger\u2019s agama (Agama boulengeri) with partial cross-amplification in\u00a0other\u00a0Agama\u00a0species"],"prefix":"10.1163","volume":"37","author":[{"given":"Duarte Vasconcelos","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"1CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"},{"name":"2Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua Campo Alegre, 4169-007 Porto, Portugal"},{"name":"3Institute of Evolutionary Biology, CSIC-Universitat Pompeu Fabra, Passeig Mar\u00edtim de la Barceloneta 37-49, 08003 Barcelona, Spain"}]},{"given":"Paulo","family":"Pereira","sequence":"additional","affiliation":[{"name":"1CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"}]},{"given":"Raquel","family":"Godinho","sequence":"additional","affiliation":[{"name":"1CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"},{"name":"2Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua Campo Alegre, 4169-007 Porto, Portugal"},{"name":"4Department of Zoology, Faculty of Sciences, University of Johannesburg, Auckland Park 2006, South Africa"}]},{"given":"Susana","family":"Lopes","sequence":"additional","affiliation":[{"name":"1CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"}]},{"given":"Guillermo","family":"Velo-Ant\u00f3n","sequence":"additional","affiliation":[{"name":"1CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"}]},{"given":"Jos\u00e9 Carlos","family":"Brito","sequence":"additional","affiliation":[{"name":"1CIBIO\/InBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"},{"name":"2Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua Campo Alegre, 4169-007 Porto, Portugal"}]}],"member":"374","reference":[{"key":"bibr1","series-title":"Biol. Rev.","first-page":"215","article-title":"Unravelling biodiversity, evolution and threats to conservation in the Sahara-Sahel","volume":"89","author":"Brito","year":"2014","unstructured":"BritoJ.C.\nGodinhoR.\nMart\u00ednez-Freir\u00edaF.\nPleguezuelosJ.M.\nRebeloH.\nSantosX.\nValeC.G.\nVelo-Ant\u00f3nG.\nBoratynskiZ.\nCarvalhoS.B.\nFerreiraS.\nGon\u00e7alvesD.V.\nSilvaT.L.\nTarrosoP.\nCamposJ.C.\nLeiteJ.V.\nNogueiraJ.\n\u00c1lvaresF.\nSilleroN.\nSowA.S.\nFahdS.\nCrochetP.A.\nCarranzaS.\n (2014): \nUnravelling biodiversity, evolution and threats to conservation in the Sahara-Sahel. \nBiol. Rev. \n89: \n215-231."},{"key":"bibr2","series-title":"Mol. Phylogenet. Evol.","first-page":"1071","article-title":"Radiation, multiple dispersal and parallelism in the skinks, Chalcides and Sphenops (Squamata: Scincidae), with comments on Scincus and Scincopus and the age of the Sahara desert","volume":"46","author":"Carranza","year":"2008","unstructured":"CarranzaS.\nArnoldE.N.\nGeniezP.\nRocaJ.\nMateoJ.A.\n (2008): \nRadiation, multiple dispersal and parallelism in the skinks, Chalcides and Sphenops (Squamata: Scincidae), with comments on Scincus and Scincopus and the age of the Sahara desert. \nMol. Phylogenet. 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Annotated inventory, with description of a new Uromastyx (Sauria: Agamidae). \nJ.\u00a0African Zool. \n110: \n22-50."},{"key":"bibr7","series-title":"Faune du Sahara. 1. Poissons, Amphibiens et Reptiles","author":"Le\u00a0Berre","year":"1989","unstructured":"Le\u00a0BerreM.\n (1989): \nFaune du Sahara. 1. Poissons, Amphibiens et Reptiles. Lechevalier, R. Chabaud, Paris."},{"key":"bibr8","series-title":"Mol. Phylogenet. Evol.","first-page":"215","article-title":"A hybrid phylogenetic-phylogenomic approach for species tree estimation in African Agama lizards with applications to biogeography, character evolution, and diversification","volume":"79","author":"Leach\u00e9","year":"2014","unstructured":"Leach\u00e9A.D.\nWagnerP.\nLinkemC.W.\nB\u00f6hmeW.\nPapenfussT.J.\nChongR.A.\nLavinB.R.\nBauerA.M.\nNielsenS.V.\nGreenbaumE.\nR\u00f6delM.O.\nSchmitzA.\nLeBretonM.\nIneichI.\nChirioL.\nOfori-BoatengC.\nEniangE.A.\nEl\u00a0DinS.B.\nLemmonA.R.\nBurbrinkF.T.\n (2014): \nA hybrid phylogenetic-phylogenomic approach for species tree estimation in African Agama lizards with applications to biogeography, character evolution, and diversification. \nMol. Phylogenet. Evol. \n79: \n215-230."},{"key":"bibr9","series-title":"Mol. Ecol. Resour.","first-page":"638","article-title":"High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries","volume":"11","author":"Malausa","year":"2011","unstructured":"MalausaT.\nGillesA.\nMegl\u00e9czE.\nBlanquartH.\nDuthoyS.\nCostedoatC.\nDubutV.\nPechN.\nCastagnone-SerenoP.\nD\u00e9lyeC.\nFeauN.\nFreyP.\nGauthierP.\nGuillemaudT.\nHazardL.\nLe\u00a0CorreV.\nLung-EscarmantB.\nMal\u00e9P.J.G.\nFerreiraS.\nMartinJ.F.\n (2011): \nHigh-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. \nMol. Ecol. Resour. \n11: \n638-644."},{"key":"bibr10","series-title":"BMC Evol. Biol.","article-title":"Conquering the Sahara and Arabian deserts: systematics and biogeography of Stenodactylus geckos (Reptilia: Gekkonidae)","volume":"12","author":"Metallinou","year":"2012","unstructured":"MetallinouM.\nArnoldE.N.\nCrochetP.\nGeniezP.\nBritoJ.C.\nLymberakisP.\nBaha El DinS.\nSindacoR.\nRobinsonM.\nCarranzaS.\n (2012): \nConquering the Sahara and Arabian deserts: systematics and biogeography of Stenodactylus geckos (Reptilia: Gekkonidae). \nBMC Evol. Biol. \n12: 258."},{"key":"bibr11","series-title":"Mol. Phylogenet. Evol.","first-page":"208","article-title":"Species on the rocks: systematics and biogeography of the rock-dwelling Ptyodactylus geckos (Squamata: Phyllodactylidae) in north Africa and Arabia","volume":"85","author":"Metallinou","year":"2015","unstructured":"MetallinouM.\n\u010cervenkaJ.\nCrochetP.A.\nKratochv\u00edlL.\nWilmsT.\nGeniezP.\nShobrakM.Y.\nBritoJ.C.\nCarranzaS.\n (2015): \nSpecies on the rocks: systematics and biogeography of the rock-dwelling Ptyodactylus geckos (Squamata: Phyllodactylidae) in north Africa and Arabia. \nMol. Phylogenet. Evol. \n85: \n208-220."},{"key":"bibr12","series-title":"Genomics","first-page":"450","article-title":"Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared fluorescence and M13 tailed primers","volume":"30","author":"Oetting","year":"1995","unstructured":"OettingW.S.\nLeeH.K.\nFlandersD.J.\nWiesnerG.L.\nSellersT.A.\nKingR.A.\n (1995): \nLinkage analysis with multiplexed short tandem repeat polymorphisms using infrared fluorescence and M13 tailed primers. \nGenomics \n30: \n450-458."},{"key":"bibr13","series-title":"Graellsia","first-page":"159","article-title":"Commented distributional list of the reptiles of Mauritania (west Africa)","volume":"62","author":"Padial","year":"2009","unstructured":"PadialJ.M.\n (2009): \nCommented distributional list of the reptiles of Mauritania (west Africa). \nGraellsia \n62: \n159-178."},{"key":"bibr14","series-title":"Bioinformatics","first-page":"2537","article-title":"GenALEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research \u2013 an update","volume":"28","author":"Peakall","year":"2012","unstructured":"PeakallR.\nSmouseP.E.\n (2012): \nGenALEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research \u2013 an update. \nBioinformatics \n28: \n2537-2539."},{"key":"bibr15","series-title":"Amphibians and Reptiles of North Africa","author":"Schleich","year":"1996","unstructured":"SchleichH.H.\nKastleW.\nKabischK.\n (1996): \nAmphibians and Reptiles of North Africa. Koeltz Scientific Publishers, Koenigstein."},{"key":"bibr16","series-title":"Nat. Rev. Genet.","first-page":"63","article-title":"The evolution of molecular markers\u00a0\u2013 just a matter of fashion?","volume":"5","author":"Schl\u00f6tterer","year":"2004","unstructured":"Schl\u00f6ttererC.\n (2004): \nThe evolution of molecular markers\u00a0\u2013 just a matter of fashion? \nNat. Rev. Genet. \n5: \n63-69."},{"key":"bibr17","series-title":"Nat. Biotechnol.","first-page":"233","article-title":"An economic method for the fluorescent labelling of PCR fragments","volume":"18","author":"Schuelke","year":"2000","unstructured":"SchuelkeM.\n (2000): \nAn economic method for the fluorescent labelling of PCR fragments. \nNat. Biotechnol. \n18: \n233-234."},{"key":"bibr18","series-title":"Ecol. Lett.","first-page":"615","article-title":"Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers","volume":"5","author":"Selkoe","year":"2006","unstructured":"SelkoeK.A.\nToonenR.J.\n (2006): \nMicrosatellites for ecologists: a practical guide to using and evaluating microsatellite markers. \nEcol. Lett. \n5: \n615-629."},{"key":"bibr19","series-title":"Org. Divers. Evol.","first-page":"295","article-title":"Where are you from, stranger? The enigmatic biogeography of north African pond turtles (Emys orbicularis)","volume":"14","author":"Stuckas","year":"2014","unstructured":"StuckasH.\nVelo-Ant\u00f3nG.\nFahdS.\nKalboussiM.\nRouagR.\nArculeoM.\nMarroneF.\nSaccoF.\nVambergerM.\nFritzU.\n (2014): \nWhere are you from, stranger? The enigmatic biogeography of north African pond turtles (Emys orbicularis). \nOrg. Divers. Evol. \n14: \n295-306."},{"key":"bibr20","series-title":"Amphibia-Reptilia","first-page":"526","article-title":"Distribution, suitable areas and conservation status of the Boulenger\u2019s agama (Agama boulengeri, Lataste 1886)","volume":"33","author":"Vale","year":"2012","unstructured":"ValeC.G.\nTarrosoP.\nCamposJ.C.\nGon\u00e7alvesD.V.\nBritoJ.C.\n (2012): \nDistribution, suitable areas and conservation status of the Boulenger\u2019s agama (Agama boulengeri, Lataste 1886). \nAmphibia-Reptilia \n33: \n526-532."},{"key":"bibr21","series-title":"Mol. Ecol. Notes","first-page":"535","article-title":"MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data","volume":"4","author":"Van\u00a0Oosterhout","year":"2004","unstructured":"Van\u00a0OosterhoutC.\nHutchinsonW.F.\nWillsD.P.M.\nShipleyP.\n (2004): \nMICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. \nMol. Ecol. Notes \n4: \n535-538."},{"key":"bibr22","series-title":"PLoS One","article-title":"Should I stay or should I go? Dispersal and population structure in small, isolated desert populations of west African crocodiles","volume":"9","author":"Velo-Ant\u00f3n","year":"2014","unstructured":"Velo-Ant\u00f3nG.\nGodinhoR.\nCamposJ.C.\nBritoJ.C.\n (2014): \nShould I stay or should I go? Dispersal and population structure in small, isolated desert populations of west African crocodiles. \nPLoS One \n9: e94626."},{"key":"bibr23","series-title":"Zool. J. Linn. Soc.","first-page":"884","article-title":"Opening a box of cryptic taxa \u2013 the first review of the north African desert lizards in the Trapelus mutabilis Merrem, 1820 complex (Squamata: Agamidae) with descriptions of new taxa","volume":"163","author":"Wagner","year":"2011","unstructured":"WagnerP.\nMelvilleJ.\nWilmsT.M.\nSchmitzA.\n (2011): \nOpening a box of cryptic taxa \u2013 the first review of the north African desert lizards in the Trapelus mutabilis Merrem, 1820 complex (Squamata: Agamidae) with descriptions of new taxa. \nZool. J. Linn. Soc. \n163: \n884-912."},{"key":"bibr24","series-title":"Electrophoresis","first-page":"2165","article-title":"Which genetic marker for which conservation genetics issue?","volume":"25","author":"Wan","year":"2004","unstructured":"WanQ.H.\nWuH.\nFujiharaT.\nFangS.G.\n (2004): \nWhich genetic marker for which conservation genetics issue? \nElectrophoresis \n25: \n2165-2176."}],"container-title":["Amphibia-Reptilia"],"original-title":[],"link":[{"URL":"https:\/\/brill.com\/view\/journals\/amre\/37\/2\/article-p246_12.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/amre\/37\/2\/article-p246_12.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/amre\/37\/2\/article-p246_12.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:14:19Z","timestamp":1761707659000},"score":1,"resource":{"primary":{"URL":"https:\/\/brill.com\/view\/journals\/amre\/37\/2\/article-p246_12.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":24,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1163\/15685381-00003048","relation":{},"ISSN":["0173-5373","1568-5381"],"issn-type":[{"type":"print","value":"0173-5373"},{"type":"electronic","value":"1568-5381"}],"subject":[],"published":{"date-parts":[[2016]]}}}