{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T08:56:37Z","timestamp":1772096197576,"version":"3.50.1"},"reference-count":98,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Insects Food Feed"],"published-print":{"date-parts":[[2024,7,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The biological valorisation of edible insects is an eco-saving approach that may increase their use for food and feeding purposes. Beetles of\n                    <jats:italic>T. molitor<\/jats:italic>\n                    are a direct waste of insect farms since their protein have a low digestibility. Currently, the degree of hydrolysis achieved with biological treatments are much lower than with chemical treatments. This work focused on the enzymatic hydrolysis of protein from\n                    <jats:italic>T. molitor<\/jats:italic>\n                    beetles using proteases. The obtained protein hydrolysate was evaluated regarding the digestibility and functional properties. Higher hydrolysis degree (HD; 47%) was obtained using a protease from\n                    <jats:italic>Aspergillus oryzae<\/jats:italic>\n                    , which efficiently separated protein from chitin. A full factorial design determined that a liquid:solid ratio (LSR) of 15 and an enzyme load of 3200 U\/g increase HD up to 78%. The protein hydrolysate showed higher digestibility (77.3%) compared to beetles\u2019 meal before (17.8%) and after defatting (25.7%). The protein hydrolysate showed a high antioxidant activity (462.1\u00a0\u00b1 7.48\u00a0\u03bcmol TE\/g DW), it was highly soluble at pH ranging between 3 and 9 and showed an emulsification activity and stability of 53.6% and 82.2%, respectively. Therefore, this bioprocess improved the use of beetles, obtaining protein with greater digestibility to be used as food.\n                  <\/jats:p>","DOI":"10.1163\/23524588-00001054","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T09:20:44Z","timestamp":1722417644000},"page":"101-113","source":"Crossref","is-referenced-by-count":0,"title":["Valorisation of Tenebrio molitor beetles: enzymes-based bioprocessing to obtain a highly digestible protein hydrolysate"],"prefix":"10.1163","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4553-5381","authenticated-orcid":true,"given":"N.","family":"Mun\u0303oz-Seijas","sequence":"first","affiliation":[{"name":"Industrial Biotechnology and Environmental Engineering Group \u201cBiotecnIA\u201d, Chemical Engineering Department, University of Vigo (Campus Ourense), 32004 Ourense, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4719-6664","authenticated-orcid":true,"given":"H.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Industrial Biotechnology and Environmental Engineering Group \u201cBiotecnIA\u201d, Chemical Engineering Department, University of Vigo (Campus Ourense), 32004 Ourense, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7655-9519","authenticated-orcid":true,"given":"D.","family":"Outeirin\u0303o","sequence":"additional","affiliation":[{"name":"Industrial Biotechnology and Environmental Engineering Group \u201cBiotecnIA\u201d, Chemical Engineering Department, University of Vigo (Campus Ourense), 32004 Ourense, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8850-4048","authenticated-orcid":true,"given":"M.G.","family":"Mora\u0301n-Aguilar","sequence":"additional","affiliation":[{"name":"Industrial Biotechnology and Environmental Engineering Group \u201cBiotecnIA\u201d, Chemical Engineering Department, University of Vigo (Campus Ourense), 32004 Ourense, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2260-3438","authenticated-orcid":true,"given":"J.M.","family":"Dom\u0131\u0301nguez","sequence":"additional","affiliation":[{"name":"Industrial Biotechnology and Environmental Engineering Group \u201cBiotecnIA\u201d, Chemical Engineering Department, University of Vigo (Campus Ourense), 32004 Ourense, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5941-3841","authenticated-orcid":true,"given":"J.M.","family":"Salgado","sequence":"additional","affiliation":[{"name":"Industrial Biotechnology and Environmental Engineering Group \u201cBiotecnIA\u201d, Chemical Engineering Department, University of Vigo (Campus Ourense), 32004 Ourense, Spain"}]}],"member":"374","reference":[{"key":"R000001","unstructured":"AOAC International, 2016. Guidelines for standard method performance requirements (Appendix F). AOAC Official Methods of Analysis. Available at: https:\/\/www.aoac.org\/wp-content\/uploads\/2019\/08\/app_f.pdf"},{"key":"R000002","series-title":"Food Research International","first-page":"24","article-title":"Extraction and physicochemical characterization of Tenebrio molitor proteins","volume":"88","author":"Azagoh, C.","unstructured":"Azagoh, C., Ducept, F., Garcia, R., Rakotozafy, L., Cuvelier, M.E., Keller, S., Lewandowski, R. and Mezdour, S., 2016. Extraction and physicochemical characterization of Tenebrio molitor proteins. Food Research International 88: 24-31."},{"key":"R000003","series-title":"Animal Welfare","article-title":"Challenges in farmed insect welfare: beyond the question of sentience","volume":"32","author":"Barrett, M.","unstructured":"Barrett, M. and Fischer, B., 2022. Challenges in farmed insect welfare: beyond the question of sentience. Animal Welfare 32: e4. https:\/\/doi.org\/10.1017\/awf.2022.5"},{"key":"R000004","series-title":"Insects","article-title":"Effects of enzymatic hydrolysis on the functional properties, antioxidant activity and protein structure of black soldier fly (Hermetia illucens)","volume":"11","author":"Batish, I.","unstructured":"Batish, I., Brits, D., Valencia, P., Miyai, C., Rafeeq, S., Xu, Y., Galanopoulos, M., Sismour, E. and Ovissipour, R., 2020. Effects of enzymatic hydrolysis on the functional properties, antioxidant activity and protein structure of black soldier fly (Hermetia illucens). Insects 11: 876. https:\/\/doi.org\/10.3390\/insects11120876"},{"key":"R000005","series-title":"Comprehensive Reviews in Food Science and Food Safety","first-page":"296","article-title":"Edible insects in a food safety and nutritional perspective: a critical review","volume":"12","author":"Belluco, S.","unstructured":"Belluco, S., Losasso, C., Maggioletti, M., Alonzi, C.C., Paoletti, M.G. and Ricci, A., 2013. Edible insects in a food safety and nutritional perspective: a critical review. Comprehensive Reviews in Food Science and Food Safety 12: 296-313."},{"key":"R000006","series-title":"Current Opinion in Food Science","article-title":"Edible insects as a sustainable alternative to food products: an insight into quality aspects of reformulated bakery and meat products","volume":"46","author":"Borges, M.M.","unstructured":"Borges, M.M., da Costa, D.V., Trombete, F.M. and Ca\u0302mara, A.K.F.I., 2022. Edible insects as a sustainable alternative to food products: an insight into quality aspects of reformulated bakery and meat products. Current Opinion in Food Science 46: 100864."},{"key":"R000007","series-title":"The Journal of Biological Chemistry","first-page":"501","article-title":"A colorimetric method for the determination of the proteolytic activity of duodenal juice","volume":"171","author":"Charney, J.","unstructured":"Charney, J. and Tomarelli, R., 1947. A colorimetric method for the determination of the proteolytic activity of duodenal juice. The Journal of Biological Chemistry 171: 501-505."},{"key":"R000008","series-title":"Food Research International","article-title":"Novel hepatoprotective peptides derived from protein hydrolysates of mealworm (Tenebrio molitor)","volume":"133","author":"Cho, H.R.","unstructured":"Cho, H.R. and Lee, S.O., 2020. Novel hepatoprotective peptides derived from protein hydrolysates of mealworm (Tenebrio molitor). Food Research International 133: 109194."},{"key":"R000009","series-title":"Nutrition Bulletin","first-page":"293","article-title":"Opportunities and hurdles of edible insects for food and feed","volume":"42","author":"Dobermann, D.","unstructured":"Dobermann, D., Swift, J.A. and Field, L.M., 2017. Opportunities and hurdles of edible insects for food and feed. Nutrition Bulletin 42: 293-308."},{"key":"R000010","series-title":"Official Journal of the European Union","first-page":"92","article-title":"Commision Regulation (EU) 2017\/893 of May 2017","volume":"2017","unstructured":"European Commission, 2017. Commision Regulation (EU) 2017\/893 of May 2017. Official Journal of the European Union 2017: 92-116."},{"key":"R000011","series-title":"Official Journal of the European Union","first-page":"48","article-title":"Commission Implementing Regulation (EU) 2022\/169 of 8 February 2022 authorising the placing on the market of frozen, dried and powder forms of yellow mealworm (Tenebrio molitor larva) as a novel food under Regulation (EU) 2015\/2283","volume":"2016","unstructured":"European Commission, 2022. Commission Implementing Regulation (EU) 2022\/169 of 8 February 2022 authorising the placing on the market of frozen, dried and powder forms of yellow mealworm (Tenebrio molitor larva) as a novel food under Regulation (EU) 2015\/2283. Official Journal of the European Union 2016: 48-119."},{"key":"R000012","series-title":"Heliyon","article-title":"Preparation and evaluation of mushroom (Lentinus edodes) and mealworm (Tenebrio molitor) as dog food attractant","volume":"6","author":"Feng, T.","unstructured":"Feng, T., Hu, Z., Tong, Y., Yao, L., Zhuang, H., Zhu, X., Song, S. and Lu, J., 2020. Preparation and evaluation of mushroom (Lentinus edodes) and mealworm (Tenebrio molitor) as dog food attractant. Heliyon 6: e05302."},{"key":"R000013","series-title":"Biofuels, Bioproducts and Biorefining","first-page":"78","article-title":"Improved lignocellulolytic enzyme production and antioxidant extraction using solid-state fermentation of olive pomace mixed with winery waste","volume":"14","author":"Filipe, D.","unstructured":"Filipe, D., Fernandes, H., Castro, C., Peres, H., Oliva-Teles, A., Belo, I. and Salgado, J.M., 2020. Improved lignocellulolytic enzyme production and antioxidant extraction using solid-state fermentation of olive pomace mixed with winery waste. Biofuels, Bioproducts and Biorefining 14: 78-91. https:\/\/doi.org\/10.1002\/bbb.2073"},{"key":"R000014","series-title":"BMC Chemistry","article-title":"Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family","volume":"14","author":"Flores, D.R.","unstructured":"Flores, D.R., Casados, L.E., Velasco, S.F., Ram\u0131\u0301rez, A.C. and Vela\u0301zquez, G., 2020. Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family. BMC Chemistry 14: 55. https:\/\/doi.org\/10.1186\/s13065-020-00707-0"},{"key":"R000015","series-title":"Foods","article-title":"Characterization of Tenebrio molitor larvae protein preparations obtained by different extraction approaches","volume":"11","author":"Gkinali, A.","unstructured":"Gkinali, A., Matsakidou, A. and Paraskevopoulou, A., 2022. Characterization of Tenebrio molitor larvae protein preparations obtained by different extraction approaches. Foods 11: 3852. https:\/\/doi.org\/10.3390\/foods11233852"},{"key":"R000016","series-title":"Food Chemistry","article-title":"New insights into the flavoring potential of cricket (Acheta domesticus) and mealworm (Tenebrio molitor) protein hydrolysates and their Maillard products","volume":"364","author":"Grossmann, K.K.","unstructured":"Grossmann, K.K., Merz, M., Appel, D., De Araujo, M.M. and Fischer, L., 2021. New insights into the flavoring potential of cricket (Acheta domesticus) and mealworm (Tenebrio molitor) protein hydrolysates and their Maillard products. Food Chemistry 364: 130336."},{"key":"R000017","series-title":"Frontiers in Nutrition","article-title":"Mealworm larvae (Tenebrio molitor) and crickets (Acheta domesticus) show high total protein in vitro digestibility and can provide good-to-excellent protein quality as determined by in vitro DIAAS","volume":"10","author":"Hammer, L.","unstructured":"Hammer, L., Moretti, D., Abb\u00fchl-Eng, L., Kandiah, P., Hilaj, N., Portmann, R. and Egger, L., 2023. Mealworm larvae (Tenebrio molitor) and crickets (Acheta domesticus) show high total protein in vitro digestibility and can provide good-to-excellent protein quality as determined by in vitro DIAAS. Frontiers in Nutrition 10: 1150581. https:\/\/doi.org\/10.3389\/fnut.2023.1150581"},{"key":"R000018","series-title":"Food Chemistry","first-page":"42","article-title":"Purification of antioxidative peptides prepared from enzymatic hydrolysates of tuna dark muscle by-product","volume":"122","author":"Hsu, K.C.","unstructured":"Hsu, K.C., 2010. Purification of antioxidative peptides prepared from enzymatic hydrolysates of tuna dark muscle by-product. Food Chemistry 122: 42-48."},{"key":"R000019","series-title":"Molecules","article-title":"The preparation and physiochemical characterization of Tenebrio molitor chitin using alcalase","volume":"28","author":"Kim, H.","unstructured":"Kim, H., Kim, H., Ahn, Y., Hong, K.B., Kim, I.W., Choi, R.Y., Suh, H.J. and Han, S.H., 2023. The preparation and physiochemical characterization of Tenebrio molitor chitin using alcalase. Molecules 28: 3254. https:\/\/doi.org\/10.3390\/molecules28073254"},{"key":"R000020","series-title":"Environmental Technology and Innovation","first-page":"77","article-title":"Extraction and characterization of chitin and chitosan from fishery waste by chemical method","volume":"3","author":"Kumari, S.","unstructured":"Kumari, S., Rath, P., Sri Hari Kumar, A. and Tiwari, T.N., 2015. Extraction and characterization of chitin and chitosan from fishery waste by chemical method. Environmental Technology and Innovation 3: 77-85."},{"key":"R000021","series-title":"Journal of Animal Science and Technology","first-page":"741","article-title":"Quality characteristics and protein digestibility of Protaetia brevitarsis larvae","volume":"62","author":"Lee, S.","unstructured":"Lee, S., Choi, Y., Jo, K., Kim, T., Yong, H.I. and Jung, S., 2020. Quality characteristics and protein digestibility of Protaetia brevitarsis larvae. Journal of Animal Science and Technology 62: 741-752."},{"key":"R000022","series-title":"Journal of Insects as Food and Feed","first-page":"393","article-title":"Protein hydrolysates from Alphitobius diaperinus and Hermetia illucens larvae treated with commercial proteases","volume":"6","author":"Leni, G.","unstructured":"Leni, G., Soetemans, L., Jacobs, J., Depraetere, S., Gianotten, N., Bastiaens, L., Caligiani, A. and Sforza, S., 2020. Protein hydrolysates from Alphitobius diaperinus and Hermetia illucens larvae treated with commercial proteases. Journal of Insects as Food and Feed 6: 393-404. https:\/\/doi.org\/10.3920\/JIFF2021.0060"},{"key":"R000023","series-title":"Annals of the Entomological Society of America","first-page":"529","article-title":"Approaches for utilizing insect protein for human consumption: effect of enzymatic hydrolysis on protein quality and functionality","volume":"112","author":"Liceaga, A.M.","unstructured":"Liceaga, A.M., 2019. Approaches for utilizing insect protein for human consumption: effect of enzymatic hydrolysis on protein quality and functionality. Annals of the Entomological Society of America 112: 529-532."},{"key":"R000024","series-title":"Current Opinion in Insect Science","first-page":"32","article-title":"Processing insects for use in the food and feed industry","volume":"48","author":"Liceaga, A.M.","unstructured":"Liceaga, A.M., 2021. Processing insects for use in the food and feed industry. Current Opinion in Insect Science 48: 32-36."},{"key":"R000025","series-title":"Food Chemistry","article-title":"Extraction, physicochemical characterization, and morphological properties of chitin and chitosan from cuticles of edible insects","volume":"343","author":"Lucas, A.J.S.","unstructured":"Lucas, A.J.S., Oreste, E.Q., Costa, H.L.G., Lo\u0301pez, H.M., Saad, C.D.M. and Prentice, C., 2021. Extraction, physicochemical characterization, and morphological properties of chitin and chitosan from cuticles of edible insects. Food Chemistry 343: 128550."},{"key":"R000026","series-title":"Sustainability","article-title":"Insect farming for feed and food production from a circular business model perspective","volume":"12","author":"Madau, F.A.","unstructured":"Madau, F.A., Arru, B., Furesi, R. and Pulina, P., 2020. Insect farming for feed and food production from a circular business model perspective. Sustainability 12: 5418. https:\/\/doi.org\/10.3390\/su12135418"},{"key":"R000027","series-title":"Italian Journal of Animal Science","first-page":"338","article-title":"In vitro crude protein digestibility of Tenebrio molitor and Hermetia illucens insect meals and its correlation with chemical composition traits","volume":"14","author":"Marono, S.","unstructured":"Marono, S., Piccolo, G., Loponte, R., Meo, C.D., Attia, Y.A., Nizza, A. and Bovera, F., 2015. In vitro crude protein digestibility of Tenebrio molitor and Hermetia illucens insect meals and its correlation with chemical composition traits. Italian Journal of Animal Science 14: 338-343."},{"key":"R000028","series-title":"Waste and Biomass Valorization","first-page":"581","article-title":"Improvement of protein bioavailability by solid-state fermentation of babassu mesocarp flour and cassava leaves","volume":"9","author":"Morales, E.M.","unstructured":"Morales, E.M., Domingos, R.N. and Angelis, D.F., 2018. Improvement of protein bioavailability by solid-state fermentation of babassu mesocarp flour and cassava leaves. Waste and Biomass Valorization 9: 581-590."},{"key":"R000029","series-title":"Advances in Food and Nutrition Research","first-page":"109","article-title":"Protein hydrolysates and biopeptides: production, biological activities, and applications in foods and health benefits. A review","author":"Nasri, M.","unstructured":"Nasri, M., 2017. Protein hydrolysates and biopeptides: production, biological activities, and applications in foods and health benefits. A review. In: Toldra\u0301, F. (ed.) Advances in Food and Nutrition Research, pp.\u00a0109-159. https:\/\/doi.org\/10.1016\/bs.afnr.2016.10.003"},{"key":"R000030","series-title":"Journal of Food Science and Technology","first-page":"3812","article-title":"Optimization of conditions for isolation of high quality chitin from shrimp processing raw byproducts using response surface methodology and its characterization","volume":"52","author":"Nidheesh, T.","unstructured":"Nidheesh, T. and Suresh, P.V., 2015. Optimization of conditions for isolation of high quality chitin from shrimp processing raw byproducts using response surface methodology and its characterization. Journal of Food Science and Technology 52: 3812-3823."},{"key":"R000031","series-title":"Innovative Food Science and Emerging Technologies","first-page":"239","article-title":"Unlocking the biological potential of proteins from edible insects through enzymatic hydrolysis: a review","volume":"43","author":"Nongonierma, A.B.","unstructured":"Nongonierma, A.B. and FitzGerald, R.J., 2017. Unlocking the biological potential of proteins from edible insects through enzymatic hydrolysis: a review. Innovative Food Science and Emerging Technologies 43: 239-252."},{"key":"R000032","series-title":"Food Bioscience","article-title":"Effects of Tenebrio molitor larvae and its protein derivatives on the antioxidant and anti-inflammatory capacities of tofu","volume":"50","author":"Oh, E.","unstructured":"Oh, E., Park, W. and Kim, Y., 2022. Effects of Tenebrio molitor larvae and its protein derivatives on the antioxidant and anti-inflammatory capacities of tofu. Food Bioscience 50: 102105."},{"key":"R000033","series-title":"Food & Function","first-page":"4376","article-title":"Evaluation of Tenebrio molitor as a source of peptides for modulating physiological processes","volume":"11","author":"Pino, F.R.","unstructured":"Pino, F.R., Ga\u0301lvez, R.P., Carpio, F.J.E. and Guadix, E.M., 2020. Evaluation of Tenebrio molitor as a source of peptides for modulating physiological processes. Food & Function 11: 4376-4386. https:\/\/doi.org\/10.1039\/D0FO00734J"},{"key":"R000034","series-title":"European Food Research and Technology","first-page":"999","article-title":"Improvement of techno-functional properties of edible insect protein from migratory locust by enzymatic hydrolysis","volume":"244","author":"Purschke, B.","unstructured":"Purschke, B., Meinlschmidt, P., Horn, C., Rieder, O. and J\u00e4ger, H., 2018. Improvement of techno-functional properties of edible insect protein from migratory locust by enzymatic hydrolysis. European Food Research and Technology 244: 999-1013."},{"key":"R000035","series-title":"Reviews in Aquaculture","first-page":"237","article-title":"Environmental consequences of using insect meal as an ingredient in aquafeeds: a systematic view","volume":"14","author":"Quang Tran, H.","unstructured":"Quang Tran, H., Van\u00a0Doan, H. and Stejskal, V., 2022. Environmental consequences of using insect meal as an ingredient in aquafeeds: a systematic view. Reviews in Aquaculture 14: 237-251."},{"key":"R000036","series-title":"Insects","article-title":"In vitro crude protein digestibility of insects: a review","volume":"13","author":"Rodr\u0131\u0301guez-Rodr\u0131\u0301guez, M.","unstructured":"Rodr\u0131\u0301guez-Rodr\u0131\u0301guez, M., Barroso, F.G., Fabrikov, D. and Sa\u0301nchez-Muros, M.J., 2022. In vitro crude protein digestibility of insects: a review. Insects 13: 682. https:\/\/doi.org\/10.3390\/insects13080682"},{"key":"R000037","series-title":"International Journal of Environmental Research and Public Health","article-title":"Consumers\u2019 acceptability and perception of edible insects as an emerging protein source","volume":"19","author":"Ros-Baro\u0301, M.","unstructured":"Ros-Baro\u0301, M., Sa\u0301nchez-Socarra\u0301s, V., Santos-Page\u0300s, M., Bach-Faig, A. and Aguilar-Mart\u0131\u0301nez, A., 2022. Consumers\u2019 acceptability and perception of edible insects as an emerging protein source. International Journal of Environmental Research and Public Health 19: 15756. https:\/\/doi.org\/10.3390\/ijerph192315756"},{"key":"R000038","series-title":"Molecular Nutrition & Food Research","first-page":"739","article-title":"Nutritional composition and safety aspects of edible insects","volume":"57","author":"Rumpold, B.A.","unstructured":"Rumpold, B.A. and Schl\u00fcter, O.K., 2013. Nutritional composition and safety aspects of edible insects. Molecular Nutrition & Food Research 57: 739-928."},{"key":"R000039","series-title":"Food & Function","first-page":"3539","article-title":"Enzymatic hydrolysis of insect Alphitobius diaperinus towards the development of bioactive peptide hydrolysates","volume":"11","author":"Sousa, P.","unstructured":"Sousa, P., Borges, S. and Pintado, M., 2020. Enzymatic hydrolysis of insect Alphitobius diaperinus towards the development of bioactive peptide hydrolysates. Food & Function 11: 3539-3548."},{"key":"R000040","series-title":"Journal of Cleaner Production","article-title":"Evaluation of Tenebrio molitor protein hydrolysates as biostimulants improving plants growth and root architecture","volume":"401","author":"Szopa, D.","unstructured":"Szopa, D., Skrzypczak, D., Izydorczyk, G., Chojnacka, K., Korczyn\u0301ski, M. and Witek-Krowiak, A., 2023. Evaluation of Tenebrio molitor protein hydrolysates as biostimulants improving plants growth and root architecture. Journal of Cleaner Production 401: 136812."},{"key":"R000041","series-title":"PLOS ONE","article-title":"Changes in the amino acid profiles and free radical scavenging activities of Tenebrio molitor larvae following enzymatic hydrolysis","volume":"13","author":"Tang, Y.","unstructured":"Tang, Y., Debnath, T., Choi, E.J., Kim, Y.W., Ryu, J.P., Jang, S., Chung, S.U., Choi, Y.J. and Kim, E.K., 2018. Changes in the amino acid profiles and free radical scavenging activities of Tenebrio molitor larvae following enzymatic hydrolysis. PLOS ONE 13: e0196218. https:\/\/doi.org\/10.1371\/journal.pone.0196218"},{"key":"R000042","series-title":"BMC Research Notes","article-title":"Housefly larvae hydrolysate: orthogonal optimization of hydrolysis, antioxidant activity, amino acid composition and functional properties","volume":"6","author":"Wang, J.","unstructured":"Wang, J., Wang, Y., Dang, X., Zheng, X. and Zhang, W., 2013. Housefly larvae hydrolysate: orthogonal optimization of hydrolysis, antioxidant activity, amino acid composition and functional properties. BMC Research Notes 6: 197. https:\/\/doi.org\/10.1186\/1756-0500-6-197"},{"key":"R000043","series-title":"Journal of Insects as Food and Feed","first-page":"21","article-title":"Extracting Tenebrio molitor protein while preventing browning: effect of pH and NaCl on protein yield","volume":"3","author":"Yi, L.","unstructured":"Yi, L., Van Boekel, M.A.J.S. and Lakemond, C.M.M., 2017. Extracting Tenebrio molitor protein while preventing browning: effect of pH and NaCl on protein yield. Journal of Insects as Food and Feed 3: 21-31. https:\/\/doi.org\/10.3920\/JIFF2016.0015"},{"key":"R000044","series-title":"Asian-Australasian Journal of Animal Sciences","first-page":"387","article-title":"Nutrient ileal digestibility evaluation of dried mealworm (Tenebrio molitor) larvae compared to three animal protein by-products in growing pigs","volume":"32","author":"Yoo, J.S.","unstructured":"Yoo, J.S., Cho, K.H., Hong, J.S., Jang, H.S., Chung, Y.H., Kwon, G.T., Shin, D.G. and Kim, Y.Y., 2019. Nutrient ileal digestibility evaluation of dried mealworm (Tenebrio molitor) larvae compared to three animal protein by-products in growing pigs. Asian-Australasian Journal of Animal Sciences 32: 387-394."},{"key":"R000045","series-title":"Foods","article-title":"Comparative characterization of protein hydrolysates from three edible insects: mealworm larvae, adult crickets, and silkworm pupae","volume":"8","author":"Yoon, S.","unstructured":"Yoon, S., Wong, N.A.K., Chae, M. and Auh, J.H., 2019. Comparative characterization of protein hydrolysates from three edible insects: mealworm larvae, adult crickets, and silkworm pupae. Foods 8: 563. https:\/\/doi.org\/10.3390\/foods8110563"},{"key":"R000046","series-title":"Food Science and Technology","first-page":"850","article-title":"Effects of wnzymatic hydrolysis and size fractionation of mealworm (Tenebrio molitor larvae) protein on antioxidant and anti-inflammatory activities and inhibition of muscle loss","volume":"3","author":"Yoon, Y.","unstructured":"Yoon, Y., Oh, E., Park, W., Kim, H.S. and Kim, Y., 2023. Effects of wnzymatic hydrolysis and size fractionation of mealworm (Tenebrio molitor larvae) protein on antioxidant and anti-inflammatory activities and inhibition of muscle loss. Food Science and Technology 3: 850-857."},{"key":"R000047","series-title":"Veterinary Sciences","article-title":"Utilization of Tenebrio molitor larvae reared with different substrates as feed ingredients in growing pigs","volume":"10","author":"Zacharis, C.","unstructured":"Zacharis, C., Bonos, E., Giannenas, I., Skoufos, I., Tzora, A., Voidarou, C.C., Tsinas, A., Fotou, K., Papadopoulos, G., Mitsagga, C., Athanassiou, C., Antonopoulou, E. and Grigoriadou, K., 2023. Utilization of Tenebrio molitor larvae reared with different substrates as feed ingredients in growing pigs. Veterinary Sciences 10: 393. https:\/\/doi.org\/10.3390\/vetsci10060393"},{"key":"R000048","series-title":"Current Research in Food Science","first-page":"1570","article-title":"Comparative study of two types of pre-extraction treatment (drying or non-drying) on physicochemical, structural and functional properties of extracted insect proteins from Tenebrio molitor larvae","volume":"5","author":"Zhang, F.","unstructured":"Zhang, F., Xu, Y., Kong, B., Chen, Q., Sun, F., Zhang, H. and Liu, Q., 2022. Comparative study of two types of pre-extraction treatment (drying or non-drying) on physicochemical, structural and functional properties of extracted insect proteins from Tenebrio molitor larvae. Current Research in Food Science 5: 1570-1580."},{"key":"R000049","series-title":"LWT","first-page":"168","article-title":"Comparison of functional properties of edible insects and protein preparations thereof","volume":"91","author":"Zielin\u0301ska, E.","unstructured":"Zielin\u0301ska, E., Karas\u0301, M. and Baraniak, B., 2018. Comparison of functional properties of edible insects and protein preparations thereof. LWT 91: 168-174. https:\/\/doi.org\/10.1016\/j.lwt.2018.01.058"},{"key":"R000001","unstructured":"AOAC International, 2016. Guidelines for standard method performance requirements (Appendix F). AOAC Official Methods of Analysis. Available at: https:\/\/www.aoac.org\/wp-content\/uploads\/2019\/08\/app_f.pdf"},{"key":"R000002","series-title":"Food Research International","first-page":"24","article-title":"Extraction and physicochemical characterization of Tenebrio molitor proteins","volume":"88","author":"Azagoh, C.","unstructured":"Azagoh, C., Ducept, F., Garcia, R., Rakotozafy, L., Cuvelier, M.E., Keller, S., Lewandowski, R. and Mezdour, S., 2016. Extraction and physicochemical characterization of Tenebrio molitor proteins. Food Research International 88: 24-31."},{"key":"R000003","series-title":"Animal Welfare","article-title":"Challenges in farmed insect welfare: beyond the question of sentience","volume":"32","author":"Barrett, M.","unstructured":"Barrett, M. and Fischer, B., 2022. Challenges in farmed insect welfare: beyond the question of sentience. Animal Welfare 32: e4. https:\/\/doi.org\/10.1017\/awf.2022.5"},{"key":"R000004","series-title":"Insects","article-title":"Effects of enzymatic hydrolysis on the functional properties, antioxidant activity and protein structure of black soldier fly (Hermetia illucens)","volume":"11","author":"Batish, I.","unstructured":"Batish, I., Brits, D., Valencia, P., Miyai, C., Rafeeq, S., Xu, Y., Galanopoulos, M., Sismour, E. and Ovissipour, R., 2020. Effects of enzymatic hydrolysis on the functional properties, antioxidant activity and protein structure of black soldier fly (Hermetia illucens). Insects 11: 876. https:\/\/doi.org\/10.3390\/insects11120876"},{"key":"R000005","series-title":"Comprehensive Reviews in Food Science and Food Safety","first-page":"296","article-title":"Edible insects in a food safety and nutritional perspective: a critical review","volume":"12","author":"Belluco, S.","unstructured":"Belluco, S., Losasso, C., Maggioletti, M., Alonzi, C.C., Paoletti, M.G. and Ricci, A., 2013. Edible insects in a food safety and nutritional perspective: a critical review. Comprehensive Reviews in Food Science and Food Safety 12: 296-313."},{"key":"R000006","series-title":"Current Opinion in Food Science","article-title":"Edible insects as a sustainable alternative to food products: an insight into quality aspects of reformulated bakery and meat products","volume":"46","author":"Borges, M.M.","unstructured":"Borges, M.M., da Costa, D.V., Trombete, F.M. and Ca\u0302mara, A.K.F.I., 2022. Edible insects as a sustainable alternative to food products: an insight into quality aspects of reformulated bakery and meat products. Current Opinion in Food Science 46: 100864."},{"key":"R000007","series-title":"The Journal of Biological Chemistry","first-page":"501","article-title":"A colorimetric method for the determination of the proteolytic activity of duodenal juice","volume":"171","author":"Charney, J.","unstructured":"Charney, J. and Tomarelli, R., 1947. A colorimetric method for the determination of the proteolytic activity of duodenal juice. The Journal of Biological Chemistry 171: 501-505."},{"key":"R000008","series-title":"Food Research International","article-title":"Novel hepatoprotective peptides derived from protein hydrolysates of mealworm (Tenebrio molitor)","volume":"133","author":"Cho, H.R.","unstructured":"Cho, H.R. and Lee, S.O., 2020. Novel hepatoprotective peptides derived from protein hydrolysates of mealworm (Tenebrio molitor). Food Research International 133: 109194."},{"key":"R000009","series-title":"Nutrition Bulletin","first-page":"293","article-title":"Opportunities and hurdles of edible insects for food and feed","volume":"42","author":"Dobermann, D.","unstructured":"Dobermann, D., Swift, J.A. and Field, L.M., 2017. Opportunities and hurdles of edible insects for food and feed. Nutrition Bulletin 42: 293-308."},{"key":"R000010","series-title":"Official Journal of the European Union","first-page":"92","article-title":"Commision Regulation (EU) 2017\/893 of May 2017","volume":"2017","unstructured":"European Commission, 2017. Commision Regulation (EU) 2017\/893 of May 2017. Official Journal of the European Union 2017: 92-116."},{"key":"R000011","series-title":"Official Journal of the European Union","first-page":"48","article-title":"Commission Implementing Regulation (EU) 2022\/169 of 8 February 2022 authorising the placing on the market of frozen, dried and powder forms of yellow mealworm (Tenebrio molitor larva) as a novel food under Regulation (EU) 2015\/2283","volume":"2016","unstructured":"European Commission, 2022. Commission Implementing Regulation (EU) 2022\/169 of 8 February 2022 authorising the placing on the market of frozen, dried and powder forms of yellow mealworm (Tenebrio molitor larva) as a novel food under Regulation (EU) 2015\/2283. Official Journal of the European Union 2016: 48-119."},{"key":"R000012","series-title":"Heliyon","article-title":"Preparation and evaluation of mushroom (Lentinus edodes) and mealworm (Tenebrio molitor) as dog food attractant","volume":"6","author":"Feng, T.","unstructured":"Feng, T., Hu, Z., Tong, Y., Yao, L., Zhuang, H., Zhu, X., Song, S. and Lu, J., 2020. Preparation and evaluation of mushroom (Lentinus edodes) and mealworm (Tenebrio molitor) as dog food attractant. Heliyon 6: e05302."},{"key":"R000013","series-title":"Biofuels, Bioproducts and Biorefining","first-page":"78","article-title":"Improved lignocellulolytic enzyme production and antioxidant extraction using solid-state fermentation of olive pomace mixed with winery waste","volume":"14","author":"Filipe, D.","unstructured":"Filipe, D., Fernandes, H., Castro, C., Peres, H., Oliva-Teles, A., Belo, I. and Salgado, J.M., 2020. Improved lignocellulolytic enzyme production and antioxidant extraction using solid-state fermentation of olive pomace mixed with winery waste. Biofuels, Bioproducts and Biorefining 14: 78-91. https:\/\/doi.org\/10.1002\/bbb.2073"},{"key":"R000014","series-title":"BMC Chemistry","article-title":"Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family","volume":"14","author":"Flores, D.R.","unstructured":"Flores, D.R., Casados, L.E., Velasco, S.F., Ram\u0131\u0301rez, A.C. and Vela\u0301zquez, G., 2020. Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family. BMC Chemistry 14: 55. https:\/\/doi.org\/10.1186\/s13065-020-00707-0"},{"key":"R000015","series-title":"Foods","article-title":"Characterization of Tenebrio molitor larvae protein preparations obtained by different extraction approaches","volume":"11","author":"Gkinali, A.","unstructured":"Gkinali, A., Matsakidou, A. and Paraskevopoulou, A., 2022. Characterization of Tenebrio molitor larvae protein preparations obtained by different extraction approaches. Foods 11: 3852. https:\/\/doi.org\/10.3390\/foods11233852"},{"key":"R000016","series-title":"Food Chemistry","article-title":"New insights into the flavoring potential of cricket (Acheta domesticus) and mealworm (Tenebrio molitor) protein hydrolysates and their Maillard products","volume":"364","author":"Grossmann, K.K.","unstructured":"Grossmann, K.K., Merz, M., Appel, D., De Araujo, M.M. and Fischer, L., 2021. New insights into the flavoring potential of cricket (Acheta domesticus) and mealworm (Tenebrio molitor) protein hydrolysates and their Maillard products. Food Chemistry 364: 130336."},{"key":"R000017","series-title":"Frontiers in Nutrition","article-title":"Mealworm larvae (Tenebrio molitor) and crickets (Acheta domesticus) show high total protein in vitro digestibility and can provide good-to-excellent protein quality as determined by in vitro DIAAS","volume":"10","author":"Hammer, L.","unstructured":"Hammer, L., Moretti, D., Abb\u00fchl-Eng, L., Kandiah, P., Hilaj, N., Portmann, R. and Egger, L., 2023. Mealworm larvae (Tenebrio molitor) and crickets (Acheta domesticus) show high total protein in vitro digestibility and can provide good-to-excellent protein quality as determined by in vitro DIAAS. Frontiers in Nutrition 10: 1150581. https:\/\/doi.org\/10.3389\/fnut.2023.1150581"},{"key":"R000018","series-title":"Food Chemistry","first-page":"42","article-title":"Purification of antioxidative peptides prepared from enzymatic hydrolysates of tuna dark muscle by-product","volume":"122","author":"Hsu, K.C.","unstructured":"Hsu, K.C., 2010. Purification of antioxidative peptides prepared from enzymatic hydrolysates of tuna dark muscle by-product. Food Chemistry 122: 42-48."},{"key":"R000019","series-title":"Molecules","article-title":"The preparation and physiochemical characterization of Tenebrio molitor chitin using alcalase","volume":"28","author":"Kim, H.","unstructured":"Kim, H., Kim, H., Ahn, Y., Hong, K.B., Kim, I.W., Choi, R.Y., Suh, H.J. and Han, S.H., 2023. The preparation and physiochemical characterization of Tenebrio molitor chitin using alcalase. Molecules 28: 3254. https:\/\/doi.org\/10.3390\/molecules28073254"},{"key":"R000020","series-title":"Environmental Technology and Innovation","first-page":"77","article-title":"Extraction and characterization of chitin and chitosan from fishery waste by chemical method","volume":"3","author":"Kumari, S.","unstructured":"Kumari, S., Rath, P., Sri Hari Kumar, A. and Tiwari, T.N., 2015. Extraction and characterization of chitin and chitosan from fishery waste by chemical method. Environmental Technology and Innovation 3: 77-85."},{"key":"R000021","series-title":"Journal of Animal Science and Technology","first-page":"741","article-title":"Quality characteristics and protein digestibility of Protaetia brevitarsis larvae","volume":"62","author":"Lee, S.","unstructured":"Lee, S., Choi, Y., Jo, K., Kim, T., Yong, H.I. and Jung, S., 2020. Quality characteristics and protein digestibility of Protaetia brevitarsis larvae. Journal of Animal Science and Technology 62: 741-752."},{"key":"R000022","series-title":"Journal of Insects as Food and Feed","first-page":"393","article-title":"Protein hydrolysates from Alphitobius diaperinus and Hermetia illucens larvae treated with commercial proteases","volume":"6","author":"Leni, G.","unstructured":"Leni, G., Soetemans, L., Jacobs, J., Depraetere, S., Gianotten, N., Bastiaens, L., Caligiani, A. and Sforza, S., 2020. Protein hydrolysates from Alphitobius diaperinus and Hermetia illucens larvae treated with commercial proteases. Journal of Insects as Food and Feed 6: 393-404. https:\/\/doi.org\/10.3920\/JIFF2021.0060"},{"key":"R000023","series-title":"Annals of the Entomological Society of America","first-page":"529","article-title":"Approaches for utilizing insect protein for human consumption: effect of enzymatic hydrolysis on protein quality and functionality","volume":"112","author":"Liceaga, A.M.","unstructured":"Liceaga, A.M., 2019. Approaches for utilizing insect protein for human consumption: effect of enzymatic hydrolysis on protein quality and functionality. Annals of the Entomological Society of America 112: 529-532."},{"key":"R000024","series-title":"Current Opinion in Insect Science","first-page":"32","article-title":"Processing insects for use in the food and feed industry","volume":"48","author":"Liceaga, A.M.","unstructured":"Liceaga, A.M., 2021. Processing insects for use in the food and feed industry. Current Opinion in Insect Science 48: 32-36."},{"key":"R000025","series-title":"Food Chemistry","article-title":"Extraction, physicochemical characterization, and morphological properties of chitin and chitosan from cuticles of edible insects","volume":"343","author":"Lucas, A.J.S.","unstructured":"Lucas, A.J.S., Oreste, E.Q., Costa, H.L.G., Lo\u0301pez, H.M., Saad, C.D.M. and Prentice, C., 2021. Extraction, physicochemical characterization, and morphological properties of chitin and chitosan from cuticles of edible insects. Food Chemistry 343: 128550."},{"key":"R000026","series-title":"Sustainability","article-title":"Insect farming for feed and food production from a circular business model perspective","volume":"12","author":"Madau, F.A.","unstructured":"Madau, F.A., Arru, B., Furesi, R. and Pulina, P., 2020. Insect farming for feed and food production from a circular business model perspective. Sustainability 12: 5418. https:\/\/doi.org\/10.3390\/su12135418"},{"key":"R000027","series-title":"Italian Journal of Animal Science","first-page":"338","article-title":"In vitro crude protein digestibility of Tenebrio molitor and Hermetia illucens insect meals and its correlation with chemical composition traits","volume":"14","author":"Marono, S.","unstructured":"Marono, S., Piccolo, G., Loponte, R., Meo, C.D., Attia, Y.A., Nizza, A. and Bovera, F., 2015. In vitro crude protein digestibility of Tenebrio molitor and Hermetia illucens insect meals and its correlation with chemical composition traits. Italian Journal of Animal Science 14: 338-343."},{"key":"R000028","series-title":"Waste and Biomass Valorization","first-page":"581","article-title":"Improvement of protein bioavailability by solid-state fermentation of babassu mesocarp flour and cassava leaves","volume":"9","author":"Morales, E.M.","unstructured":"Morales, E.M., Domingos, R.N. and Angelis, D.F., 2018. Improvement of protein bioavailability by solid-state fermentation of babassu mesocarp flour and cassava leaves. Waste and Biomass Valorization 9: 581-590."},{"key":"R000029","series-title":"Advances in Food and Nutrition Research","first-page":"109","article-title":"Protein hydrolysates and biopeptides: production, biological activities, and applications in foods and health benefits. A review","author":"Nasri, M.","unstructured":"Nasri, M., 2017. Protein hydrolysates and biopeptides: production, biological activities, and applications in foods and health benefits. A review. In: Toldra\u0301, F. (ed.) Advances in Food and Nutrition Research, pp.\u00a0109-159. https:\/\/doi.org\/10.1016\/bs.afnr.2016.10.003"},{"key":"R000030","series-title":"Journal of Food Science and Technology","first-page":"3812","article-title":"Optimization of conditions for isolation of high quality chitin from shrimp processing raw byproducts using response surface methodology and its characterization","volume":"52","author":"Nidheesh, T.","unstructured":"Nidheesh, T. and Suresh, P.V., 2015. Optimization of conditions for isolation of high quality chitin from shrimp processing raw byproducts using response surface methodology and its characterization. Journal of Food Science and Technology 52: 3812-3823."},{"key":"R000031","series-title":"Innovative Food Science and Emerging Technologies","first-page":"239","article-title":"Unlocking the biological potential of proteins from edible insects through enzymatic hydrolysis: a review","volume":"43","author":"Nongonierma, A.B.","unstructured":"Nongonierma, A.B. and FitzGerald, R.J., 2017. Unlocking the biological potential of proteins from edible insects through enzymatic hydrolysis: a review. Innovative Food Science and Emerging Technologies 43: 239-252."},{"key":"R000032","series-title":"Food Bioscience","article-title":"Effects of Tenebrio molitor larvae and its protein derivatives on the antioxidant and anti-inflammatory capacities of tofu","volume":"50","author":"Oh, E.","unstructured":"Oh, E., Park, W. and Kim, Y., 2022. Effects of Tenebrio molitor larvae and its protein derivatives on the antioxidant and anti-inflammatory capacities of tofu. Food Bioscience 50: 102105."},{"key":"R000033","series-title":"Food & Function","first-page":"4376","article-title":"Evaluation of Tenebrio molitor as a source of peptides for modulating physiological processes","volume":"11","author":"Pino, F.R.","unstructured":"Pino, F.R., Ga\u0301lvez, R.P., Carpio, F.J.E. and Guadix, E.M., 2020. Evaluation of Tenebrio molitor as a source of peptides for modulating physiological processes. Food & Function 11: 4376-4386. https:\/\/doi.org\/10.1039\/D0FO00734J"},{"key":"R000034","series-title":"European Food Research and Technology","first-page":"999","article-title":"Improvement of techno-functional properties of edible insect protein from migratory locust by enzymatic hydrolysis","volume":"244","author":"Purschke, B.","unstructured":"Purschke, B., Meinlschmidt, P., Horn, C., Rieder, O. and J\u00e4ger, H., 2018. Improvement of techno-functional properties of edible insect protein from migratory locust by enzymatic hydrolysis. European Food Research and Technology 244: 999-1013."},{"key":"R000035","series-title":"Reviews in Aquaculture","first-page":"237","article-title":"Environmental consequences of using insect meal as an ingredient in aquafeeds: a systematic view","volume":"14","author":"Quang Tran, H.","unstructured":"Quang Tran, H., Van\u00a0Doan, H. and Stejskal, V., 2022. Environmental consequences of using insect meal as an ingredient in aquafeeds: a systematic view. Reviews in Aquaculture 14: 237-251."},{"key":"R000036","series-title":"Insects","article-title":"In vitro crude protein digestibility of insects: a review","volume":"13","author":"Rodr\u0131\u0301guez-Rodr\u0131\u0301guez, M.","unstructured":"Rodr\u0131\u0301guez-Rodr\u0131\u0301guez, M., Barroso, F.G., Fabrikov, D. and Sa\u0301nchez-Muros, M.J., 2022. In vitro crude protein digestibility of insects: a review. Insects 13: 682. https:\/\/doi.org\/10.3390\/insects13080682"},{"key":"R000037","series-title":"International Journal of Environmental Research and Public Health","article-title":"Consumers\u2019 acceptability and perception of edible insects as an emerging protein source","volume":"19","author":"Ros-Baro\u0301, M.","unstructured":"Ros-Baro\u0301, M., Sa\u0301nchez-Socarra\u0301s, V., Santos-Page\u0300s, M., Bach-Faig, A. and Aguilar-Mart\u0131\u0301nez, A., 2022. Consumers\u2019 acceptability and perception of edible insects as an emerging protein source. International Journal of Environmental Research and Public Health 19: 15756. https:\/\/doi.org\/10.3390\/ijerph192315756"},{"key":"R000038","series-title":"Molecular Nutrition & Food Research","first-page":"739","article-title":"Nutritional composition and safety aspects of edible insects","volume":"57","author":"Rumpold, B.A.","unstructured":"Rumpold, B.A. and Schl\u00fcter, O.K., 2013. Nutritional composition and safety aspects of edible insects. Molecular Nutrition & Food Research 57: 739-928."},{"key":"R000039","series-title":"Food & Function","first-page":"3539","article-title":"Enzymatic hydrolysis of insect Alphitobius diaperinus towards the development of bioactive peptide hydrolysates","volume":"11","author":"Sousa, P.","unstructured":"Sousa, P., Borges, S. and Pintado, M., 2020. Enzymatic hydrolysis of insect Alphitobius diaperinus towards the development of bioactive peptide hydrolysates. Food & Function 11: 3539-3548."},{"key":"R000040","series-title":"Journal of Cleaner Production","article-title":"Evaluation of Tenebrio molitor protein hydrolysates as biostimulants improving plants growth and root architecture","volume":"401","author":"Szopa, D.","unstructured":"Szopa, D., Skrzypczak, D., Izydorczyk, G., Chojnacka, K., Korczyn\u0301ski, M. and Witek-Krowiak, A., 2023. Evaluation of Tenebrio molitor protein hydrolysates as biostimulants improving plants growth and root architecture. Journal of Cleaner Production 401: 136812."},{"key":"R000041","series-title":"PLOS ONE","article-title":"Changes in the amino acid profiles and free radical scavenging activities of Tenebrio molitor larvae following enzymatic hydrolysis","volume":"13","author":"Tang, Y.","unstructured":"Tang, Y., Debnath, T., Choi, E.J., Kim, Y.W., Ryu, J.P., Jang, S., Chung, S.U., Choi, Y.J. and Kim, E.K., 2018. Changes in the amino acid profiles and free radical scavenging activities of Tenebrio molitor larvae following enzymatic hydrolysis. PLOS ONE 13: e0196218. https:\/\/doi.org\/10.1371\/journal.pone.0196218"},{"key":"R000042","series-title":"BMC Research Notes","article-title":"Housefly larvae hydrolysate: orthogonal optimization of hydrolysis, antioxidant activity, amino acid composition and functional properties","volume":"6","author":"Wang, J.","unstructured":"Wang, J., Wang, Y., Dang, X., Zheng, X. and Zhang, W., 2013. Housefly larvae hydrolysate: orthogonal optimization of hydrolysis, antioxidant activity, amino acid composition and functional properties. BMC Research Notes 6: 197. https:\/\/doi.org\/10.1186\/1756-0500-6-197"},{"key":"R000043","series-title":"Journal of Insects as Food and Feed","first-page":"21","article-title":"Extracting Tenebrio molitor protein while preventing browning: effect of pH and NaCl on protein yield","volume":"3","author":"Yi, L.","unstructured":"Yi, L., Van Boekel, M.A.J.S. and Lakemond, C.M.M., 2017. Extracting Tenebrio molitor protein while preventing browning: effect of pH and NaCl on protein yield. Journal of Insects as Food and Feed 3: 21-31. https:\/\/doi.org\/10.3920\/JIFF2016.0015"},{"key":"R000044","series-title":"Asian-Australasian Journal of Animal Sciences","first-page":"387","article-title":"Nutrient ileal digestibility evaluation of dried mealworm (Tenebrio molitor) larvae compared to three animal protein by-products in growing pigs","volume":"32","author":"Yoo, J.S.","unstructured":"Yoo, J.S., Cho, K.H., Hong, J.S., Jang, H.S., Chung, Y.H., Kwon, G.T., Shin, D.G. and Kim, Y.Y., 2019. Nutrient ileal digestibility evaluation of dried mealworm (Tenebrio molitor) larvae compared to three animal protein by-products in growing pigs. Asian-Australasian Journal of Animal Sciences 32: 387-394."},{"key":"R000045","series-title":"Foods","article-title":"Comparative characterization of protein hydrolysates from three edible insects: mealworm larvae, adult crickets, and silkworm pupae","volume":"8","author":"Yoon, S.","unstructured":"Yoon, S., Wong, N.A.K., Chae, M. and Auh, J.H., 2019. Comparative characterization of protein hydrolysates from three edible insects: mealworm larvae, adult crickets, and silkworm pupae. Foods 8: 563. https:\/\/doi.org\/10.3390\/foods8110563"},{"key":"R000046","series-title":"Food Science and Technology","first-page":"850","article-title":"Effects of wnzymatic hydrolysis and size fractionation of mealworm (Tenebrio molitor larvae) protein on antioxidant and anti-inflammatory activities and inhibition of muscle loss","volume":"3","author":"Yoon, Y.","unstructured":"Yoon, Y., Oh, E., Park, W., Kim, H.S. and Kim, Y., 2023. Effects of wnzymatic hydrolysis and size fractionation of mealworm (Tenebrio molitor larvae) protein on antioxidant and anti-inflammatory activities and inhibition of muscle loss. Food Science and Technology 3: 850-857."},{"key":"R000047","series-title":"Veterinary Sciences","article-title":"Utilization of Tenebrio molitor larvae reared with different substrates as feed ingredients in growing pigs","volume":"10","author":"Zacharis, C.","unstructured":"Zacharis, C., Bonos, E., Giannenas, I., Skoufos, I., Tzora, A., Voidarou, C.C., Tsinas, A., Fotou, K., Papadopoulos, G., Mitsagga, C., Athanassiou, C., Antonopoulou, E. and Grigoriadou, K., 2023. Utilization of Tenebrio molitor larvae reared with different substrates as feed ingredients in growing pigs. Veterinary Sciences 10: 393. https:\/\/doi.org\/10.3390\/vetsci10060393"},{"key":"R000048","series-title":"Current Research in Food Science","first-page":"1570","article-title":"Comparative study of two types of pre-extraction treatment (drying or non-drying) on physicochemical, structural and functional properties of extracted insect proteins from Tenebrio molitor larvae","volume":"5","author":"Zhang, F.","unstructured":"Zhang, F., Xu, Y., Kong, B., Chen, Q., Sun, F., Zhang, H. and Liu, Q., 2022. Comparative study of two types of pre-extraction treatment (drying or non-drying) on physicochemical, structural and functional properties of extracted insect proteins from Tenebrio molitor larvae. Current Research in Food Science 5: 1570-1580."},{"key":"R000049","series-title":"LWT","first-page":"168","article-title":"Comparison of functional properties of edible insects and protein preparations thereof","volume":"91","author":"Zielin\u0301ska, E.","unstructured":"Zielin\u0301ska, E., Karas\u0301, M. and Baraniak, B., 2018. Comparison of functional properties of edible insects and protein preparations thereof. LWT 91: 168-174. https:\/\/doi.org\/10.1016\/j.lwt.2018.01.058"}],"container-title":["Journal of Insects as Food and Feed"],"original-title":[],"link":[{"URL":"https:\/\/brill.com\/view\/journals\/jiff\/11\/1\/article-p101_6.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/jiff\/11\/1\/article-p101_6.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/jiff\/11\/1\/article-p101_6.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T07:59:27Z","timestamp":1772092767000},"score":1,"resource":{"primary":{"URL":"https:\/\/brill.com\/view\/journals\/jiff\/11\/1\/article-p101_6.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,16]]},"references-count":98,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1163\/23524588-00001054","relation":{},"ISSN":["2352-4588"],"issn-type":[{"value":"2352-4588","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,16]]}}}