{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T22:50:32Z","timestamp":1778107832119,"version":"3.51.4"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1987,8,1]],"date-time":"1987-08-01T00:00:00Z","timestamp":554774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1987,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Patients with tuberculous pleural effusions may show cutaneous anergy to tuberculin purified-protein derivative (PPD). This phenomenon has been attributed either to preferential sequestration of antigen-specific T-lymphocytes to the pleural space or to the presence of suppressor monocytes in the blood. In 2 patients with primary tuberculous infection involving the pleura and with skin anergy to PPD, the in vitro proliferation of pleural and peripheral blood T-cells to PPD was evaluated. Although eripheral blood T-cells were not reactive, pleural T-cells showed a marked proliferative response to PPD. At least in the first patient, the lack of proliferation of circulating T-cells could not be related to the presence of suppressor monocytes. Interestingly, after 4 to 8 wk of specific chemotherapy, PPD skin test became positive and the blood T-lymphocytes responded in vitro to the same antigen. Pleural T-lymphocytes were used to generate long-term, PPD-specific T-cell lines and could be maintained in vitro for more than 3 months with repeated cycles of stimulation. The pleural and the blood T-lymphocytes and the T-cell lines were also characterized phenotypically: although the majority of the T-lymphocytes present in the pleural space after Mycobacterium tuberculosis infection were Leu-3-positive (helper T-cells), T-cell lines proliferating in response to PPD Included high numbers of Leu-2-positive cells (suppressor\/cytotoxic T-cells). These data suggest that early skin anergy in tuberculous pleurisy may be associated with sequestration of PPD-reactive T-lymphocytes in the pleural spaces involving both Leu-2- and Leu-3-positive T-cells.<\/jats:p>","DOI":"10.1164\/ajrccm\/136.3.575","type":"journal-article","created":{"date-parts":[[2011,7,21]],"date-time":"2011-07-21T18:39:14Z","timestamp":1311273554000},"page":"575-579","source":"Crossref","is-referenced-by-count":69,"title":["Tuberculous Pleural Effusions: Evidence for Selective Presence of PPD-Specific T-Lymphocytes at Site of Inflammation in the Early Phase of the Infection"],"prefix":"10.1093","volume":"136","author":[{"given":"Giovanni A.","family":"Rossi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Balbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizio","family":"Manca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1987,8,1]]},"reference":[{"key":"p_6","first-page":"303","volume":"27","year":"1977","journal-title":"Clin Exp Immunol"},{"key":"p_11","first-page":"42","volume":"125","year":"1982","journal-title":"Am Rev Respir Dis"},{"key":"p_19","first-page":"932","volume":"89","year":"1978","journal-title":"Med"},{"key":"p_21","volume":"312","author":"Hospital N","year":"1985","journal-title":"J Med"},{"key":"p_36","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.4049\/jimmunol.121.6.2573","volume":"121","year":"1978","journal-title":"J Immunol"},{"key":"p_38","doi-asserted-by":"publisher","DOI":"10.1378\/chest.77.1.4"},{"key":"p_41","first-page":"77","volume":"2","year":"1968","journal-title":"Invest [Suppl.)"},{"key":"p_63","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198212233072601"},{"key":"p_64","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198108203050804"},{"key":"p_68","first-page":"839","volume":"2","year":"1981","journal-title":"Lancet"},{"key":"p_73","doi-asserted-by":"publisher","DOI":"10.1378\/chest.85.4.514"},{"key":"p_78","first-page":"1086","volume":"133","year":"1986","journal-title":"Am Rev Respir Dis"},{"key":"p_90","doi-asserted-by":"publisher","DOI":"10.1159\/000229793"},{"key":"p_94","doi-asserted-by":"publisher","DOI":"10.1159\/000230265"},{"key":"p_99","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/140.1.12"},{"key":"p_108","doi-asserted-by":"publisher","DOI":"10.1378\/chest.89.4.530"}],"container-title":["American Review of Respiratory Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/136\/3\/575\/67746610\/ajrccm_136_3_575.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/136\/3\/575\/67746610\/ajrccm_136_3_575.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T05:23:06Z","timestamp":1775193786000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/136\/3\/575\/8557720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,8,1]]},"references-count":16,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1987,8,1]]},"published-print":{"date-parts":[[1987,8,1]]}},"URL":"https:\/\/doi.org\/10.1164\/ajrccm\/136.3.575","relation":{},"ISSN":["0003-0805"],"issn-type":[{"value":"0003-0805","type":"print"}],"subject":[],"published-other":{"date-parts":[[1987,8]]},"published":{"date-parts":[[1987,8,1]]}}}