{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T08:08:12Z","timestamp":1775203692085,"version":"3.50.1"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1989,1,1]],"date-time":"1989-01-01T00:00:00Z","timestamp":599616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1989,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Reproducibility of methacholine inhalation tests (MIT) over a 2-wk period has been established in adult populations, but similar studies demonstrating reproducibility in children are lacking. We set out to establish the reproducibility of MIT in children as a prerequisite for a study of the natural history of airway hyperreactivity in asthmatic children. Most inhalation testing is done in persons with mild asthma because the recommended time interval for the withholding of medications prior to bronchial challenge is poorly tolerated by more labile asthmatics. In order to evaluate asthmatics with more severe disease, we modified a standardized method of methacholine inhalation to include a three-tier pretest medication regimen and investigated the reproducibility of this MIT protocol in 11 children as young as 6 yr of age. The three tiers were designed to keep baseline FEV1 \u2265 70% predicted since diminished baseline airway caliber may affect MIT results. Eight of the 11 children were bronchodilator-dependent, and two of the eight also required inhaled steroids.<\/jats:p>\n                  <jats:p>Eleven children (6 to 13 yr of age) underwent MIT, between December and March, 1 day, 1 wk, and 1 month after an initial test. The PD20FEV1 using cumulative breath units (BU) were compared. The range of PD20FEV1 in the 11 children was 0.27 to 14.4 BU, with nine subjects classified as severe (PD20FEV1 &amp;lt; 2.5 BU). We found a high degree of reproducibility of MIT. The intertest correlation coefficient (r) was 0.98 after 1 day, 0.95 after 1 wk, and 0.96 after 1 month. We conclude that our three-tier pretest medication regimen allows children as young as 6 yr of age, with moderate to severe asthma, to undergo successive MIT. This protocol should prove useful in allowing serial studies of airway hyperreactivity in asthmatic children.<\/jats:p>","DOI":"10.1164\/ajrccm\/139.1.67","type":"journal-article","created":{"date-parts":[[2011,7,22]],"date-time":"2011-07-22T00:33:11Z","timestamp":1311294791000},"page":"67-72","source":"Crossref","is-referenced-by-count":14,"title":["A Protocol for Performing Reproducible Methacholine Inhalation Tests in Children with Moderate to Severe Asthma"],"prefix":"10.1093","volume":"139","author":[{"given":"Michael E.","family":"Weiss","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Wheeler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peyton","family":"Eggleston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"N. F.","family":"Adkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1989,1,1]]},"reference":[{"key":"p_3","first-page":"452","volume":"115","year":"1965","journal-title":"Med"},{"key":"p_18","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(75)90107-4"},{"key":"p_25","unstructured":"matic patients. J Allergy Clin Immunol 1981; 67:"},{"key":"p_28","doi-asserted-by":"publisher","DOI":"10.1136\/thx.33.6.705"},{"key":"p_31","doi-asserted-by":"publisher","DOI":"10.1136\/thx.41.2.128"},{"key":"p_35","doi-asserted-by":"publisher","DOI":"10.1136\/thx.38.7.523"},{"key":"p_44","doi-asserted-by":"publisher","DOI":"10.1136\/thx.38.7.516"},{"key":"p_51","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(82)90147-6"},{"key":"p_55","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(84)90279-3"},{"key":"p_59","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1164\/arrd.1986.134.5.994","volume":"134","year":"1986","journal-title":"Respir Dis"},{"key":"p_62","first-page":"609","volume":"76","year":"1985","journal-title":"Clin Immunol"},{"key":"p_66","first-page":"221","volume":"129","year":"1984","journal-title":"Respir Dis"},{"key":"p_78","first-page":"911","volume":"117","year":"1978","journal-title":"Dis"},{"key":"p_81","doi-asserted-by":"publisher","DOI":"10.1136\/thx.38.4.258"},{"key":"p_111","first-page":"111","volume":"80","year":"1987","journal-title":"Clin Immunol"},{"key":"p_124","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(79)80075-X"},{"key":"p_127","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1177\/014107688107400604","volume":"74","year":"1981","journal-title":"J R Soc Med"},{"key":"p_131","first-page":"105","volume":"58","year":"1987","journal-title":"Allerg"},{"key":"p_142","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(81)90012-9"},{"key":"p_168","first-page":"911","volume":"117","year":"1978","journal-title":"Dis"},{"key":"p_169","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(87)90235-1"},{"key":"p_178","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1164\/arrd.1984.130.2.193","volume":"130","year":"1984","journal-title":"Rev Respir Dis"},{"key":"p_183","first-page":"214","volume":"76","year":"1985","journal-title":"Clin Immunol"},{"key":"p_187","first-page":"162","volume":"64","year":"1979","journal-title":"Clin Immunol"},{"key":"p_191","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1976.03270090021019"},{"key":"p_195","first-page":"331","volume":"71","year":"1983","journal-title":"Clin Immunol"}],"container-title":["American Review of Respiratory Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/139\/1\/67\/67750371\/ajrccm_139_1_67.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/139\/1\/67\/67750371\/ajrccm_139_1_67.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:03:03Z","timestamp":1775196183000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/139\/1\/67\/8560332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,1,1]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1989,1,1]]},"published-print":{"date-parts":[[1989,1,1]]}},"URL":"https:\/\/doi.org\/10.1164\/ajrccm\/139.1.67","relation":{},"ISSN":["0003-0805"],"issn-type":[{"value":"0003-0805","type":"print"}],"subject":[],"published-other":{"date-parts":[[1989,1]]},"published":{"date-parts":[[1989,1,1]]}}}