{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:24:27Z","timestamp":1790616267830,"version":"4.1.0"},"reference-count":9,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1989,9,1]],"date-time":"1989-09-01T00:00:00Z","timestamp":620611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1989,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lymphocyte infiltration of central airway epithelium was evaluated in 13 normal nonsmoking subjects (Group 1), in 11 smokers without clinical signs of chronic bronchitis (Group 2), and in 34 patients who were smokers with chronic bronchitis and mild airflow limitation (Group 3). Bronchial samples were obtained through fiberoptic bronchoscopy. Murine monoclonal antibodies directed against cell-surface antigens and an immunoperoxidase technique were used on cryostat sections to label in situ the following lymphocyte populations: T-lymphocytes (CD3+), helper\/inducer T-cells (CD4+), suppressor\/cytotoxic T-cells (CD8+) and B-lymphocytes (leu 12+). Virtually no B-cells were found in central airway epithelium from subjects of any group. Conversely, consistent infiltration of epithelial layers with T-lymphocytes of both subsets was observed in all subjects, with a constant predominance of CD8+ over CD4+ cells. For any T-cell marker, differences between mean scores from Group 1 and Group 2 subjects were not statistically significant. On the other hand, mean lymphocyte numbers of both subsets were found increased in patients from Group 3 compared with subjects from the two other groups: statistically significant differences were observed for CD3+, CD4+, and CD8+ cells (p &amp;lt; 0.001). Furthermore, lymphocyte scores at two different airway generations were compared in some patients from Groups 2 and 3, and a significant positive correlation was observed. These results suggest that T-lymphocyte infiltration of central airway epithelium (1) may be a naturally occurring phenomenon that is amplified in the airways of smokers with chronic bronchitis, and (22) may represent the counterpart to the intraepithelial population of the intestine. Because a majority of the CD8+ cells that populate the intestinal epithelium lack pan-T-cell markers, the possibility that the CD8+ cell population in the airway layer is heterogeneous remains to be explored.<\/jats:p>","DOI":"10.1164\/ajrccm\/140.3.737","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T17:04:30Z","timestamp":1365095070000},"page":"737-742","source":"Crossref","is-referenced-by-count":79,"title":["Intraepithelial T-Lymphocyte Subsets in the Airways of Normal Subjects and of Patients with Chronic Bronchitis"],"prefix":"10.1093","volume":"140","author":[{"given":"Michel","family":"Fournier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Lebargy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois Le Roy","family":"Ladurie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Lenormand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Pariente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1989,9,1]]},"reference":[{"key":"p_4","first-page":"1","year":"1984","journal-title":"York: McGraw-Hill"},{"key":"p_12","first-page":"219","volume":"52","year":"1983","journal-title":"Exp Immunol"},{"key":"p_17","first-page":"245","volume":"135","year":"1987","journal-title":"Rev Respir Dis"},{"key":"p_18","first-page":"5","volume":"131","author":"Le Roy Ladurie F","year":"1985","journal-title":"Am Rev Respir Dis"},{"key":"p_23","doi-asserted-by":"publisher","DOI":"10.1177\/29.4.6166661"},{"key":"p_26","first-page":"3773","volume":"10","year":"1986","journal-title":"J Immunol"},{"key":"p_46","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.4049\/jimmunol.130.6.2615","volume":"130","year":"1983","journal-title":"J Immunol"},{"key":"p_53","first-page":"249","volume":"41","year":"1980","journal-title":"Immunology"},{"key":"p_63","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4049\/jimmunol.131.1.132","volume":"131","year":"1983","journal-title":"J Immunol"}],"container-title":["American Review of Respiratory Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/140\/3\/737\/67751841\/ajrccm_140_3_737.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/140\/3\/737\/67751841\/ajrccm_140_3_737.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:08:26Z","timestamp":1775196506000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/140\/3\/737\/8558965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,9,1]]},"references-count":9,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1989,9,1]]},"published-print":{"date-parts":[[1989,9,1]]}},"URL":"https:\/\/doi.org\/10.1164\/ajrccm\/140.3.737","relation":{},"ISSN":["0003-0805"],"issn-type":[{"value":"0003-0805","type":"print"}],"subject":[],"published-other":{"date-parts":[[1989,9]]},"published":{"date-parts":[[1989,9,1]]}}}