{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:41:55Z","timestamp":1787168515777,"version":"build-2736575974"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1993,2,1]],"date-time":"1993-02-01T00:00:00Z","timestamp":728524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Standard microbiologic techniques were compared with a rapid diagnostic method based on the amplification by polymerase chain reaction (PCR) of a fragment of the IS6110 insertion element (present in multiple copies in the Mycobacterium tuberculosis genome) for the detection of M. tuberculosis in specimens obtained from children diagnosed as having primary tuberculosis on clinical grounds. Two (n = 7) or three (n = 15) gastric aspirates were obtained from the 22 children with primary tuberculosis. All specimens were negative for mycobacteria by acid-fast staining and culture. When DNA was purified from the clinical specimens and aliquots of each sample were amplified in duplicate, 15 of 59 (25%) specimens gave at least one positive result. Increasing beyond two the number of times that samples were tested did not appreciably improve sensitivity. Testing multiple samples from the same individual increased the diagnostic yield. Thus, when three different samples from the same subject were tested two times each, two or more positive results were obtained from 9 of 15 children with primary tuberculosis but 0 of 17 control subjects. Samples from children with symptoms, recent contact with patients with active tuberculosis, vesicular tuberculin responses, or abnormal chest radiographs were more frequently positive than those from patients whose only manifestation of tuberculosis was a positive (but not vesicular) tuberculin response. Thus, M. tuberculosis DNA can be detected by PCR in gastic aspirates of many children with primary tuberculosis, despite that specimens from these patients are negative by culture. Multiple samples must be tested to optimize the diagnostic yield.<\/jats:p>","DOI":"10.1164\/ajrccm\/147.2.420","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T17:40:26Z","timestamp":1365097226000},"page":"420-424","source":"Crossref","is-referenced-by-count":62,"title":["Diagnosis of Primary Tuberculosis in Children by Amplification and Detection of Mycobacterial DNA"],"prefix":"10.1093","volume":"147","author":[{"given":"Catherine","family":"Pierre","sequence":"first","affiliation":[{"name":"INSERM Unit\u00e9 82, Facult\u00e9 de M\u00e9decine Xavier Bichat, Paris, the Service de P\u00e9diatrie and the Laboratoire de Microbiologie, H\u00f4pital Louis Mourier, Colombes, and the Service de M\u00e9decine Interne, H\u00f4pital Bichat-Claude Bernard, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Olivier","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 82, Facult\u00e9 de M\u00e9decine Xavier Bichat, Paris, the Service de P\u00e9diatrie and the Laboratoire de Microbiologie, H\u00f4pital Louis Mourier, Colombes, and the Service de M\u00e9decine Interne, H\u00f4pital Bichat-Claude Bernard, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Lecossier","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 82, Facult\u00e9 de M\u00e9decine Xavier Bichat, Paris, the Service de P\u00e9diatrie and the Laboratoire de Microbiologie, H\u00f4pital Louis Mourier, Colombes, and the Service de M\u00e9decine Interne, H\u00f4pital Bichat-Claude Bernard, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Boussougant","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 82, Facult\u00e9 de M\u00e9decine Xavier Bichat, Paris, the Service de P\u00e9diatrie and the Laboratoire de Microbiologie, H\u00f4pital Louis Mourier, Colombes, and the Service de M\u00e9decine Interne, H\u00f4pital Bichat-Claude Bernard, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Yeni","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 82, Facult\u00e9 de M\u00e9decine Xavier Bichat, Paris, the Service de P\u00e9diatrie and the Laboratoire de Microbiologie, H\u00f4pital Louis Mourier, Colombes, and the Service de M\u00e9decine Interne, H\u00f4pital Bichat-Claude Bernard, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan J.","family":"Hance","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 82, Facult\u00e9 de M\u00e9decine Xavier Bichat, Paris, the Service de P\u00e9diatrie and the Laboratoire de Microbiologie, H\u00f4pital Louis Mourier, Colombes, and the Service de M\u00e9decine Interne, H\u00f4pital Bichat-Claude Bernard, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1993,2,1]]},"reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-198804000-00008"},{"key":"p_2","first-page":"735","author":"Chernick V","year":"1990","journal-title":"Philadelphia: W. B. Saunders"},{"key":"p_3","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/S0031-3955(16)36465-3","volume":"35","author":"Starke JR","year":"1988","journal-title":"Pediatr Clin North Am"},{"key":"p_4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00233.x"},{"key":"p_5","first-page":"1068","volume":"2","author":"Brlsson-Noel A","year":"1989","journal-title":"Lancet"},{"key":"p_6","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/144.5.1160"},{"key":"p_7","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.5.977"},{"key":"p_8","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1128\/JCM.28.9.2051-2058.1990","volume":"28","author":"Hermans PWM","year":"1990","journal-title":"J Clin Microbiol"},{"key":"p_9","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1128\/JCM.28.9.1877-1880.1990","volume":"28","author":"Pao CC","year":"1990","journal-title":"J Clin Microbiol"},{"key":"p_10","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1128\/JCM.28.3.513-518.1990","volume":"28","author":"Patel RJ","year":"1990","journal-title":"J Clin Microbiol"},{"key":"p_11","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1128\/JCM.28.9.1913-1917.1990","volume":"28","author":"Plikaytis BB","year":"1990","journal-title":"J Clin Microbiol"},{"key":"p_13","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)93328-7"},{"key":"p_14","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1128\/JCM.28.11.2437-2441.1990","volume":"28","author":"De Wit 0","year":"1990","journal-title":"J Clin Microbiol"},{"key":"p_15","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1128\/JCM.29.4.712-717.1991","volume":"29","author":"Pierre C","year":"1990","journal-title":"J Clin Microbiol"},{"key":"p_16","first-page":"676","volume":"100","author":"Tacquet A","year":"1961","journal-title":"Ann Inst Pasteur"},{"key":"p_17","doi-asserted-by":"publisher","DOI":"10.1038\/339237a0"},{"key":"p_18","doi-asserted-by":"publisher","DOI":"10.1038\/343027a0"},{"key":"p_19","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.1.188"},{"key":"p_20","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)55023-6"},{"key":"p_21","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.21.6409"},{"key":"p_22","first-page":"209","volume":"92","author":"Baess I","year":"1984","journal-title":"Acta Pathol Microbiol Immunol Scand B"},{"key":"p_23","doi-asserted-by":"publisher","DOI":"10.1136\/thx.46.2.96"},{"key":"p_24","first-page":"67","volume":"173","author":"Peres PJ","year":"1988","journal-title":"Rev Fr Lab"},{"key":"p_25","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90492-8"},{"key":"p_26","doi-asserted-by":"publisher","DOI":"10.1136\/thx.47.4.265"},{"key":"p_27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.1.99"}],"container-title":["American Review of Respiratory Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/147\/2\/420\/67756272\/ajrccm_147_2_420.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/147\/2\/420\/67756272\/ajrccm_147_2_420.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:33:25Z","timestamp":1775198005000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/147\/2\/420\/8556417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2,1]]},"references-count":26,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1993,2,1]]},"published-print":{"date-parts":[[1993,2,1]]}},"URL":"https:\/\/doi.org\/10.1164\/ajrccm\/147.2.420","relation":{},"ISSN":["0003-0805"],"issn-type":[{"value":"0003-0805","type":"print"}],"subject":[],"published-other":{"date-parts":[[1993,2]]},"published":{"date-parts":[[1993,2,1]]}}}