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In order to explore whether endogenously produced adenosine may contribute to the pathophysiologic aspects of asthma, we measured adenosine concentrations in bronchoalveolar lavage (BAL) fluid in seven subjects with asthma, eight asymptomatic cigarette smokers, and eight normal subjects. The mean concentration of adenosine in BAL fluid from the normal subjects was 0.72 \u00b1 0.16 \u00b5M. Subjects with asthma and cigarette smokers had significantly increased concentrations of adenosine in BAL fluid, 2.55 \u00b1 0.50 and 1.89 \u00b1 0.50 \u00b5M, respectively. Corrected for the dilution that occurs as a result of the lavage procedure, mean epithelial lining fluid adenosine concentrations were 60 \u00b1 13 \u00b5M in normal subjects, 193 \u00b1 58 \u00b5M in asthmatics, and 155 \u00b1 56 \u00b5M in smokers. Adenosine concentrations were positively correlated with the protein content of the lavage fluid (r = 0.79). Inhalation of nebulized adenosine in the subjects with asthma provoked a 20% reduction in lung function at concentrations 4- to 195-fold higher than was present in the epithelial lining fluid of the same individuals. The presence of increased BAL adenosine concentrations in asthmatics and in cigarette smokers suggests that adenosine may be a nonspecific marker for inflammation in the lung. The demonstration of physiologically relevant concentrations of adenosine in airway fluids of subjects with bronchial hyperreactivity to inhaled adenosine provides evidence for a role of endogenous adenosine in provoking bronchoconstriction in asthma.<\/jats:p>","DOI":"10.1164\/ajrccm\/148.1.91","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T17:45:18Z","timestamp":1365097518000},"page":"91-97","source":"Crossref","is-referenced-by-count":250,"title":["Adenosine in Bronchoalveolar Lavage Fluid in Asthma"],"prefix":"10.1093","volume":"148","author":[{"given":"Albert G.","family":"Driver","sequence":"first","affiliation":[{"name":"Departments of Medicine and Pharmacology, East Carolina University School of Medicine, Greenville, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia A.","family":"Kukoly","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Pharmacology, East Carolina University School of Medicine, Greenville, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahid","family":"Ali","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Pharmacology, East Carolina University School of Medicine, Greenville, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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