{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T10:55:24Z","timestamp":1774695324918,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1995,12,1]],"date-time":"1995-12-01T00:00:00Z","timestamp":817776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The role of allergen-specific sIgE+ B cells in the development of airway hyperresponsiveness to electrical field stimulation was examined in a murine model of allergic sensitization. Ovalbumin (OVA)-specific B cells (OVA+) were isolated from mice that were sensitized to aerosolized OVA. The OVA+ B cell population was shown to be distinct from the remaining, non-OVA-responsive B cells (OVA-). There was a high frequency of sIgE+ B cells and a low frequency of sIgG+ B cells in the OVA+ population compared with the OVA- population, where the ratio was reversed. Although both populations produced immunoglobulin in vitro, only the OVA+ cells secreted anti-OVA antibodies. Transfer of 10(6) OVA+ B cells or as few as 5 x 10(4) OVA+\/sIgE+ B cells was able to transfer the capability for anti-OVA IgE synthesis and cutaneous reactivity to OVA in naive recipients. Exposure to OVA via the airways in addition to transfer of OVA+ B cells was necessary for development of airway hyperresponsiveness, whereas recipients challenged with an irrelevant allergen, ragweed, had normal airway function. Transfer of up to 10(7) OVA- B cells failed to induce production of anti-OVA IgE. Despite production of polyclonal IgE, recipients of OVA- B cells did not develop airway hyperresponsiveness after OVA challenge. We conclude that both allergen-specific IgE production and local challenge via the airways with specific allergen are necessary to change airway function in this model.<\/jats:p>","DOI":"10.1164\/ajrccm.152.6.8520735","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T17:53:31Z","timestamp":1365098011000},"page":"1765-1773","source":"Crossref","is-referenced-by-count":19,"title":["Transfer of Immediate Hypersensitivity and Airway Hyperresponsiveness by IgE-Positive B Cells"],"prefix":"10.1093","volume":"152","author":[{"given":"G","family":"Lack","sequence":"first","affiliation":[{"name":"Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Oshiba","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K L","family":"Bradley","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J E","family":"Loader","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Amran","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G L","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E W","family":"Gelfand","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1995,12,1]]},"container-title":["American journal of respiratory and critical care medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/152\/6\/1765\/67629683\/ajrccm_152_6_1765.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/152\/6\/1765\/67629683\/ajrccm_152_6_1765.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T10:40:34Z","timestamp":1774694434000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/152\/6\/1765\/8554014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1995,12,1]]},"published-print":{"date-parts":[[1995,12,1]]}},"URL":"https:\/\/doi.org\/10.1164\/ajrccm.152.6.8520735","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1995,12]]},"published":{"date-parts":[[1995,12,1]]}}}