{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T07:49:08Z","timestamp":1774684148073,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1996,8,1]],"date-time":"1996-08-01T00:00:00Z","timestamp":838857600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1996,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We report the activity of recombinant human surfactant apoprotein-C (rSP-C[Cys]2) and various phospholipids in a preterm rabbit model of respiratory distress syndrome (RDS). Mixtures of rSP-C(Cys)2 and certain phospholipids had similar activity (lung compliance and lung pressure-volume behavior) to rabbit surfactant in this model. The activity of rSP-C(Cys)2 was maximal at 1 mol% protein and varied significantly with the phospholipid composition. Chemically synthesized SP-C had similar activity to rSP-C(Cys)2. Deletion of six amino-terminal residues did not affect function. Substitution of cysteines and cysteine6 with adjacent serines (rSP-C[Ser]2) by site-specific mutagenesis minimized aggregation of rSP-C but did not affect activity. Palmitoylation of cysteine5 and cysteine6 in rSP-C (rSP-C[C16]2) did not enhance the activity of rSP-C(Cys)2. We conclude that bacterial expression is a practical source of functional SP-C, and that nonacylated forms of SP-C may be useful adjuvants to phospholipids in the treatment of RDS and possibly other forms of acute lung injury.<\/jats:p>","DOI":"10.1164\/ajrccm.154.2.8756826","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T17:53:52Z","timestamp":1365098032000},"page":"484-490","source":"Crossref","is-referenced-by-count":42,"title":["Lung Function in Premature Rabbits Treated With Recombinant Human Surfactant Protein-C"],"prefix":"10.1093","volume":"154","author":[{"given":"S","family":"Hawgood","sequence":"first","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Yukitake","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Schlueter","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Brown","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"White","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Buckley","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Lesikar","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Benson","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, University of California, San Francisco 94143-0130, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1996,8,1]]},"container-title":["American journal of respiratory and critical care medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/154\/2\/484\/67615171\/ajrccm_154_2_484.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/154\/2\/484\/67615171\/ajrccm_154_2_484.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T06:54:47Z","timestamp":1774680887000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/154\/2\/484\/8552638"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1996,8,1]]},"published-print":{"date-parts":[[1996,8,1]]}},"URL":"https:\/\/doi.org\/10.1164\/ajrccm.154.2.8756826","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1996,8]]},"published":{"date-parts":[[1996,8,1]]}}}