{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T06:49:21Z","timestamp":1774680561001,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1997,2,1]],"date-time":"1997-02-01T00:00:00Z","timestamp":854755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The human pleural space is lined by a single layer of mesothelial cells, the intracellular pH (pH(i)) of which has never been investigated. In the present study, the intrinsic buffering power of H+ ions (beta(i)) and the pH(i) regulatory systems were investigated in primary cultures of human pleural mesothelial cells (PMCs) with microspectrofluorimetry. We found: (1) that at the resting pH(i), the beta(i) was low and increased as the pH(i) decreased; (2) that the pH(i) recovery was largely inhibited either with Na+-free medium or nominally HCO3 free medium containing ethyl-isopropyl amiloride (EIPA); (3) a 4-4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS)-sensitive, Na+\/HCO3-dependent, but Cl(-)-independent acid extrusion mechanism in CO2\/HCO3 buffer; and (4) that in the same buffer, a DIDS- sensitive but Na+-independent alkalosis was induced by intracellular Cl- depletion. We therefore conclude that at least three membrane pH(i) regulators are involved in regulating the pH(i) in PMCs, these being the EIPA-sensitive Na+-H+ exchanger; a novel electroneutral, DIDS-sensitive Na+-HCO3 cotransporter; and the DIDS-sensitive Cl(-)-HCO3 exchanger. Furthermore, under physiologic conditions, the Na+-HCO3 cotransporter plays a more important role in extrusion of excess intracellular H+ ions than does the Na+-H+ exchanger.<\/jats:p>","DOI":"10.1164\/ajrccm.155.2.9032200","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T17:54:36Z","timestamp":1365098076000},"page":"597-602","source":"Crossref","is-referenced-by-count":3,"title":["Intracellular pH Regulation in Cultured Human Pleural Mesothelial Cells"],"prefix":"10.1093","volume":"155","author":[{"given":"Y S","family":"Liaw","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P C","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C J","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S H","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K T","family":"Luh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y J","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M L","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, R.O.C"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1997,2,1]]},"container-title":["American journal of respiratory and critical care medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/155\/2\/597\/67611240\/ajrccm_155_2_597.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/155\/2\/597\/67611240\/ajrccm_155_2_597.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T05:25:36Z","timestamp":1774675536000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/155\/2\/597\/8551983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1997,2,1]]},"published-print":{"date-parts":[[1997,2,1]]}},"URL":"https:\/\/doi.org\/10.1164\/ajrccm.155.2.9032200","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1997,2]]},"published":{"date-parts":[[1997,2,1]]}}}