{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:37:43Z","timestamp":1790789863948,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Artificial mechanical ventilation represents a major cause of iatrogenic lung damage in intensive care. It is largely unknown which mediators, if any, contribute to the onset of such complications. We investigated whether stress caused by artificial mechanical ventilation leads to induction, synthesis, and release of cytokines or eicosanoids from lung tissue. We used the isolated perfused and ventilated mouse lung where frequent perfusate sampling allows determination of mediator release into the perfusate. Hyperventilation was executed with either negative (NPV) or positive pressure ventilation (PPV) at a transpulmonary pressure that was increased 2.5-fold above normal. Both modes of hyperventilation resulted in an approximately 1.75-fold increased expression of tumor necrosis factor \u03b1 (TNF \u03b1 ) and interleukin-6 (IL-6) mRNA, but not of cyclooxygenase-2 mRNA. After switching to hyperventilation, prostacyclin release into the perfusate increased almost instantaneously from 19 \u00b1 17 pg\/ min to 230 \u00b1 160 pg\/min (PPV) or 115 \u00b1 87 pg\/min (NPV). The enhancement in TNF \u03b1 and IL-6 production developed more slowly. In control lungs after 150 min of perfusion and ventilation, TNF \u03b1 and IL-6 production was 23 \u00b1 20 pg\/min and 330 \u00b1 210 pg\/min, respectively. In lungs hyperventilated for 150 min, TNF \u03b1 and IL-6 production were increased to 287 \u00b1 180 pg\/min and more than 1,000 pg\/ min, respectively. We conclude that artificial ventilation might cause pulmonary and systemic adverse reactions by inducing the release of mediators into the circulation. von Bethmann AN, Brasch F, N\u00fcsing R, Vogt K, Volk HD, M\u00fcller K-M, Wendel A, Uhlig S. Hyperventilation induces release of cytokines from perfused mouse lung.<\/jats:p>","DOI":"10.1164\/ajrccm.157.1.9608052","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T13:30:55Z","timestamp":1365082255000},"page":"263-272","source":"Crossref","is-referenced-by-count":264,"title":["Hyperventilation Induces Release of Cytokines from Perfused Mouse Lung"],"prefix":"10.1093","volume":"157","author":[{"given":"ALEXANDER NEPOMUK","family":"von BETHMANN","sequence":"first","affiliation":[{"name":"Biochemical Pharmacology, University of Konstanz, Konstanz; Professional Associations' Hospital Bergmannsheil University Hospital, Institute of Pathology, Bochum; Medical Center of Pediatrics, University Hospital Marburg, Marburg; and Institute for Medical Immunology, Charit\u00e8, Humboldt University, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FRANK","family":"BRASCH","sequence":"additional","affiliation":[{"name":"Biochemical Pharmacology, University of Konstanz, Konstanz; Professional Associations' Hospital Bergmannsheil University Hospital, Institute of Pathology, Bochum; Medical Center of Pediatrics, University Hospital Marburg, Marburg; and Institute for Medical Immunology, Charit\u00e8, Humboldt University, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ROLF","family":"N\u00dcSING","sequence":"additional","affiliation":[{"name":"Biochemical Pharmacology, University of Konstanz, Konstanz; Professional Associations' Hospital Bergmannsheil University Hospital, Institute of Pathology, Bochum; Medical Center of Pediatrics, University Hospital Marburg, Marburg; and Institute for Medical Immunology, Charit\u00e8, Humboldt University, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KATHRIN","family":"VOGT","sequence":"additional","affiliation":[{"name":"Biochemical Pharmacology, University of Konstanz, Konstanz; Professional Associations' Hospital Bergmannsheil University Hospital, Institute of Pathology, Bochum; Medical Center of Pediatrics, University Hospital Marburg, Marburg; and Institute for Medical Immunology, Charit\u00e8, Humboldt University, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HANS D.","family":"VOLK","sequence":"additional","affiliation":[{"name":"Biochemical Pharmacology, University of Konstanz, Konstanz; Professional Associations' Hospital Bergmannsheil University Hospital, Institute of Pathology, Bochum; Medical Center of Pediatrics, University Hospital Marburg, Marburg; and Institute for Medical Immunology, Charit\u00e8, Humboldt University, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KLAUS-MICHAEL","family":"M\u00dcLLER","sequence":"additional","affiliation":[{"name":"Biochemical Pharmacology, University of Konstanz, Konstanz; Professional Associations' Hospital Bergmannsheil University Hospital, Institute of Pathology, Bochum; Medical Center of Pediatrics, University Hospital Marburg, Marburg; and Institute for Medical Immunology, Charit\u00e8, Humboldt University, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ALBRECHT","family":"WENDEL","sequence":"additional","affiliation":[{"name":"Biochemical Pharmacology, University of Konstanz, Konstanz; Professional Associations' Hospital Bergmannsheil University Hospital, Institute of Pathology, Bochum; Medical Center of Pediatrics, University Hospital Marburg, Marburg; and Institute for Medical Immunology, Charit\u00e8, Humboldt University, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"STEFAN","family":"UHLIG","sequence":"additional","affiliation":[{"name":"Biochemical Pharmacology, University of Konstanz, Konstanz; Professional Associations' Hospital Bergmannsheil University Hospital, Institute of Pathology, Bochum; Medical Center of Pediatrics, University Hospital Marburg, Marburg; and Institute for Medical Immunology, Charit\u00e8, Humboldt University, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1998,1,1]]},"reference":[{"key":"2026032709095726800_B1"},{"key":"2026032709095726800_B2","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1378\/chest.91.4.608","article-title":"Barotrauma: pathophysiology, risk factors and prevention.","volume":"91","author":"Haake","year":"1987","journal-title":"Chest"},{"key":"2026032709095726800_B3","first-page":"H499","article-title":"Noninvasive measurement of ascending aortic blood velocity in mice.","volume":"268","author":"Hartley","year":"1995","journal-title":"Am. 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